TLR5 Antibody (JM10-88)

Western Blot: TLR5 Antibody (JM10-88) [NBP2-67205] - Analysis of TLR5 on Hela cells lysates using anti-TLR5 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: TLR5 Antibody (JM10-88) [NBP2-67205] - Analysis of paraffin-embedded mouse testis tissue using anti-TLR5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TLR5 Antibody (JM10-88) [NBP2-67205] - Analysis of paraffin-embedded human spleen tissue using anti-TLR5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TLR5 Antibody (JM10-88) [NBP2-67205] - Analysis of paraffin-embedded mouse prostate tissue using anti-TLR5 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TLR5 Antibody (JM10-88) [NBP2-67205] - Analysis of paraffin-embedded mouse stomack tissue using anti-TLR5 antibody. Counter stained with hematoxylin.

Product Discontinued

TLR5 Antibody (JM10-88) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human TLR5 aa 238-424 / 858. (SwissProt: O60602 Human; SwissProt: Q9JLF7 Mouse; Unigene: 198962 Rat)
• Localization: Membrane.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TLR5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TLR5 Antibody (JM10-88)

• MGC126430
• MGC126431
• SLEB1FLJ10052
• TIL-3
• TIL3Toll/interleukin-1 receptor-like protein 3
• TLR5
• toll-like receptor 5

Background

The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR5