Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Product Discontinued

Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line Summary

• Description: The TLR10 reporter line may be useful for TLR10-based functional studies. The functional activity of the NF-kB/SEAP reporter in the TLR10 stable cell line has been established and validated through PMA activation of the NF-kB pathway (Figure 1). Hence, the cell line may be useful for reseasrchers screening for putative TLR10 ligands activating the NF-kB pathway.
  
  Any specific ligand for TLR10 is still unknown. However, TLR10 can indeed activate the NF-kB signaling pathway through direct association with the common Toll/IL-1 receptor domain adapter, MyD88, according to a recent study utilizing a chimeric CD4/TLR10 molecule. TLR10 expression in the TLR10/NF-kB/SEAPorter HEK 293 cell line has been validated by Western blotting (Fig. 2) and flow cytometry (Fig. 3).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: The TLR10/NF-kB/SEAPorter HEK 293 cell line is a stably transfected cell line which expresses full-length human Toll-like receptor 10 (TLR10) with an N-terminal HA tag and the secreted alkaline phosphatase (SEAP) reporter gene under the transcriptional control of an NF-kB response element.
• Target Species: Human
• Specificity: TLR10/NF-kB/SEAP
• Selection Agent: Blasticidin and G418.
• Growth Properties: Adherent Morphology: Epithelial
• RCL Type: NF-kB/SEAP - (SEAPorter™)
• Reporter Gene: Secreted alkaline phosphatase (SEAP)
• Host: HEK293
• Gene: TLR10

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Western Blot
• Readout System:
  • Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) NBP2-25285

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin + 0.5 mg/ml G418 (Geneticin).

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the TLR10 reporter line.
- Wash hands after handling the TLR10 reporter line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR10 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

• CD290 antigen
• CD290
• MGC104967
• MGC126398
• MGC126399
• TLR10
• toll-like receptor 10

Background

Toll-like receptors (TLRs) are signaling molecules that recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. These proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors. TLR10 is highly homologous to TLRs 1 and 6 and is most highly expressed in lymphoid tissues. Most recently genetic variation in TLR10 has been associated with contributing to asthma risk. It should be noted that TLR10 exists in mice only as a pseudogene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLR10