Human TLR1 Stable Cell Line

Product Discontinued

Human TLR1 Stable Cell Line Summary

• Description: The TLR1 stable cell line can be used for TLR1 flow cytometric calibration and detection control. TLR1 expression has been validated by Western blotting (fig. 1) and flow cytometry (fig. 2).
  
  Contents: 3~4 x 10^6 cells
  Biosafety Level: 2
• Immunogen: TLR1 stable cell line expresses full-length human Toll-like receptor 1 (TLR1) with an N-terminal HA tag.
• Target Species: Human
• Specificity: TLR1
• Selection Agent: Blasticidin
• RCL Type: Stable Cell Line
• Host: HEK293
• Gene: TLR1

Applications/Dilutions

• Readout System:
  • NFkB Secreted Alkaline Phosphatase Reporter Assay Kit (Colorimetric) NBP2-25286
  • Cell Surface Staining Flow Cytometry Kit NBP2-26247

Packaging, Storage & Formulations

• Storage: Store in gas phase of liquid nitrogen.
• Reconstitution Instructions: Complete Growth Medium: DMEM with 4.5 g/L glucose + 10% FBS + 4 mM L-glutamine + 1 mM sodium pyruvate + 100 units/ml penicillin + 0.1 mg/ml streptomycin + 10 ug/ml blasticidin.

Details for Array

• Type: Cell

Notes

Assume all cultures are hazardous since they may harbor latent viruses or other organisms that are uncharacterized. The following safety precautions should be observed.
- Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
- No eating, drinking or smoking while handling the TLR1 stable line.
- Wash hands after handling the TLR1 stable line and before leaving the lab.
- Decontaminate work surface with disinfectant or 70% ethanol before and after working with cells.
- All waste should be considered hazardous.
- Dispose of all liquid waste after each experiment and treat with bleach.

Alternate Names for Human TLR1 Stable Cell Line

• CD281 antigen
• CD281
• DKFZp547I0610
• DKFZp564I0682
• KIAA0012MGC126311
• MGC104956
• MGC126312
• rsc786
• TIL
• TIL. LPRS5
• TLR1
• Toll/interleukin-1 receptor-like protein
• toll-like receptor 1

Background

Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. The TLRs act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors so the organism can respond to potential infection. TLR1 is co-expressed with TLR2 on myeloid cells of the innate immune systems in lymphoid tissue such as monocytes and dendritic cells where they form heterodimers that can recognize triacylated lipoproteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Reporter Cell Lines are guaranteed for 1 year from date of receipt.

Publications for TLR1 Reporter Cell Line (NBP2-26264)(1)

Publication using NBP2-26264

• Mukherjee S, Biswas R, Biswas T. Alternative TLRs are stimulated by bacterial ligand to induce TLR2-unresponsive colon cell response. Cell Signal. 2013-08-01 [PMID: 23665374] (IP/WB)
  
  Details:
  TLR Stable cell lines cited in Fig 2C for IP/WB:1. TLR1 Stable Cell Line (IML-201)2. TLR2 Stable Cell Line (IML-202)3. TLR4 Stable Cell Line (IML-204)Note: The cell lines were exposed to porin and IP'd with a TLR1, TLR2, or TLR4 antibody, respectively, fo

Bioinformatics

• Gene Symbol: TLR1