TIM-1/KIM-1/HAVCR Antibody

Images

 
Genetic Strategies: Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. ...read more
Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - in Human Uterus cells. Dilution 20 ug/mL.
Immunohistochemistry-Paraffin: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Analysis of human uterus tissue using anti-TIM-1 antibody (3809) at 10 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 ...read more
Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate ...read more
Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP ...read more
Independent Antibodies: Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TIM-1/KIM-1/HAVCR Antibody Summary

Immunogen
Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1. Amino Acid Squence: TNGTHVTYRKDTRYKL
Specificity
Human TIM-1/KIM-1/HAVCR has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD). This antibody detects human isoform b precursor. Mouse TIM-1/KIM-1/HAVCR has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1/KIM-1/HAVCR has only one isoform identified so far (307aa, 34kD).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HAVCR1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-8 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Knockdown Validated
Application Notes
ICC/IF reactivity reported in (PMID: 27214555).
Theoretical MW
44 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
TIM-1/KIM-1/HAVCR Peptide (NBP1-76701PEP)
Publications
Read Publications using
NBP1-76701 in the following applications:

Reactivity Notes

Mouse reactivity reported in (PMID: 29369823). Rat reactivity reported in (PMID: 30066041).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for TIM-1/KIM-1/HAVCR Antibody

  • CD365
  • HAVCR1
  • HAVCR-1
  • HAVCRT cell immunoglobin domain and mucin domain protein 1
  • hepatitis A virus cellular receptor 1
  • Kidney injury molecule 1
  • KIM1
  • KIM-1
  • T-cell immunoglobulin and mucin domain-containing protein 1
  • TIM1
  • TIM-1
  • TIM-1TIM
  • TIM1TIMD-1
  • TIMD1T-cell membrane protein 1

Background

The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TIM-1/KIM-1/HAVCR Antibody (NBP1-76701)(13)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 4 applications: ICC/IF, IHC, IHC-P, WB.


Filter By Application
ICC/IF
(3)
IHC
(4)
IHC-P
(1)
WB
(6)
All Applications
Filter By Species
Human
(2)
Mouse
(10)
Rat
(1)
All Species
Showing Publications 1 - 10 of 13. Show All 13 Publications.
Publications using NBP1-76701 Applications Species
Kang HM, Lim JH, Noh KH et al. Effective reconstruction of functional organotypic kidney spheroid for in vitro nephrotoxicity studies Sci Rep Nov 26 2019 [PMID: 31772214] (ICC/IF, Mouse) ICC/IF Mouse
Samodelov SL, Visentin M, Gai Z et al. Renal glycosuria as a novel early sign of colistin-induced kidney damage in mice Antimicrob. Agents Chemother. 2019 Oct 07 [PMID: 31591120] (IHC, Mouse) IHC Mouse
Song MF, Yang Y, Yi ZW et al. Sema 3A as a biomarker of the activated mTOR pathway during hexavalent chromium-induced acute kidney injury. Toxicol. Lett. Sep 17 2018 [PMID: 30236763] (WB, Human) WB Human
Sharma M, Naura AS, Singla SK. Modulatory effect of 4-phenyl butyric acid on hyperoxaluria-induced renal injury and inflammation Mol. Cell. Biochem. Jul 31 2018 [PMID: 30066041] (IHC-P, Rat) IHC-P Rat
Qing X, Chinenov Y, Redecha P et al. iRhom2 promotes lupus nephritis through TNF-a and EGFR signaling J. Clin. Invest. 2018 Jan 25 [PMID: 29369823] (Mouse) Mouse
Trudu M, Schaeffer C, Riba M et al. Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations Sci Rep 2017 Aug 07 [PMID: 28785050] (WB, Mouse) WB Mouse
Morrison MC, Yakala GK, Liang W et al. Protective effect of rosiglitazone on kidney function in high-fat challenged human-CRP transgenic mice: a possible role for adiponectin and miR-21. Sci Rep. 2017 Jun 06 [PMID: 28588299] (IHC, Mouse) IHC Mouse
Weber HM, Tsurkan MV, Magno V et al. Heparin-based hydrogels induce human renal tubulogenesis in vitro. Acta Biomater May 17 2017 [PMID: 28526628]
Pei L, Solis G, Nguyen MT et al. Paracellular epithelial sodium transport maximizes energy efficiency in the kidney J. Clin. Invest Jul 1 2016 [PMID: 27214555] (ICC/IF, Mouse) ICC/IF Mouse
Tang W H Wilson, Wang Zeneng, Kennedy David J et al. Gut microbiota-dependent trimethylamine N-oxide (TMAO) pathway contributes to both development of renal insufficiency and mortality risk in chronic kidney disease. Circulation Research 2015 [PMID: 25599331] (WB, Mouse) WB Mouse
Show All 13 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for TIM-1/KIM-1/HAVCR Antibody (NBP1-76701). (Showing 1 - 1 of 1 FAQs).

  1. I am using  KIM-1 antibody NBP1-76701 on NIH-3T3 cell lysate. What is the molecular weight of KIM1? Also, do you suggest any positive control cell line or tissue for WB testing of KIM1?
    • The predicted MWt of KIM-1 is ~38.7 kDa and in our Western Blot validation of TIM-1/KIM-1/HAVCR antibody [NBP1-76701] at 1 ug/mL dilution, we observed a specific band around this position in 3T3 cell lysate. The other cell type which is known to express KIM1 is stimulated T-cells wherein it is expressed during primary antigen stimulation (PMID 11725301). According to protein atlas database (ENSG00000113249), this protein is expressed in Kidney & gastrointestinal tissues also. 

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Bioinformatics

Gene Symbol HAVCR1
Entrez
Uniprot