Genetic Strategies: Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) Loading: 15 ug of HeLa whole cell lysates per lane. ...read more
Immunocytochemistry/ Immunofluorescence: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - in Human Uterus cells. Dilution 20 ug/mL.
Immunohistochemistry-Paraffin: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Analysis of human uterus tissue using anti-TIM-1 antibody (3809) at 10 ug/ml.Tissue was fixed with formaldehyde and blocked with 10% serum for 1 ...read more
Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate ...read more
Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of NIH/3T3 cell lysates. Antibodies: TIM-1 3809 (8 ug/mL), overnight incubation at 4 C in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP ...read more
Independent Antibodies: Western Blot: TIM-1/KIM-1/HAVCR Antibody [NBP1-76701] - Loading: 15 ug of lysates per lane. Antibodies: TIM-1 3809 (8 ug/mL), TIM-13811 (1 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ...read more
Antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-1. The immunogen is located within amino acids 50 - 100 of TIM-1. Amino Acid Squence: TNGTHVTYRKDTRYKL
Human TIM-1/KIM-1/HAVCR has 5 isoforms, including isoform a precursor (364aa, 39kD), isoform a (364aa, 39kD), isoform b precursor (401aa, 44kD), isoform b (359aa, 39kD), and isoform c (359aa, 39kD). This antibody detects human isoform b precursor. Mouse TIM-1/KIM-1/HAVCR has 3 isoforms, including isoform a precursor (305aa, 33kD), isoform b precursor (282aa, 31kD) and isoform b (282aa, 31kD). Rat TIM-1/KIM-1/HAVCR has only one isoform identified so far (307aa, 34kD).
Peptide affinity purified
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44 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Mouse reactivity reported in (PMID: 29369823). Rat reactivity reported in (PMID: 30066041).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Peptide affinity purified
Alternate Names for TIM-1/KIM-1/HAVCR Antibody
HAVCRT cell immunoglobin domain and mucin domain protein 1
hepatitis A virus cellular receptor 1
Kidney injury molecule 1
T-cell immunoglobulin and mucin domain-containing protein 1
TIMD1T-cell membrane protein 1
The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for TIM-1/KIM-1/HAVCR Antibody (NBP1-76701). (Showing 1 - 1 of 1 FAQs).
I am using KIM-1 antibody NBP1-76701 on NIH-3T3 cell lysate. What is the molecular weight of KIM1? Also, do you suggest any positive control cell line or tissue for WB testing of KIM1?
The predicted MWt of KIM-1 is ~38.7 kDa and in our Western Blot validation of TIM-1/KIM-1/HAVCR antibody [NBP1-76701] at 1 ug/mL dilution, we observed a specific band around this position in 3T3 cell lysate. The other cell type which is known to express KIM1 is stimulated T-cells wherein it is expressed during primary antigen stimulation (PMID 11725301). According to protein atlas database (ENSG00000113249), this protein is expressed in Kidney & gastrointestinal tissues also.
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