TIF1 alpha Antibody (PCRP-TRIM24-1B12) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-08786] - Immunofluorescent Analysis of PFA-fixed MCF7 cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by IgG-CF488 (green), counterstained with phalloidin.

Flow Cytometry: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-08786] - Flow Cytometric Analysis of PFA-fixed HeLa cells. TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12) followed by goat anti- Mouse IgG-CF488 (blue); isotype control (red).

Protein Array: TIF1 alpha Antibody (PCRP-TRIM24-1B12) [NBP3-08786] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIF1 alpha Mouse Monoclonal Antibody (PCRP-TRIM24-1B12).

• Reactivity: Hu
• Applications: Flow, ICC/IF, MA
• Clone: PCRP-TRIM24-1B12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

TIF1 alpha Antibody (PCRP-TRIM24-1B12) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07464).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human TIF1 alpha protein (Uniprot: O15164)
• Localization: Nucleus, Cytoplasm. Colocalizes with sites of active transcription.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM24
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Protein Array
• Application Notes: Optimal dilution for a specific application should be determined.
• Theoretical MW: 117 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for TIF1 alpha Antibody (PCRP-TRIM24-1B12) - Azide and BSA Free

• E3 ubiquitin-protein ligase TRIM24
• EC 6.3.2
• EC 6.3.2.-
• hTIF1
• PTC6
• RING finger protein 82
• RNF82
• RNF82Tif1a
• TIF1 alpha
• TIF1
• TIF1A
• TIF1-alpha
• TIF1ATIF1ALPHA
• TIF1TF1A
• transcription intermediary factor 1-alpha
• transcriptional intermediary factor 1
• TRIM24
• tripartite motif containing 24
• tripartite motif-containing 24
• Tripartite motif-containing protein 24

Background

TIF1 alpha is encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM24