TIF1 alpha Antibody (2F2)

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2. Analysis of TRIM24 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of monoclonal antibody to TRIM24 on formalin-fixed paraffin-embedded human testis. Antibody concentration 6 ug/ml.

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - TRIM24 monoclonal antibody (M01), clone 2F2 Analysis of TRIM24 expression in IMR-32.

Western Blot: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 expression in transfected 293T cell line by TRIM24 monoclonal antibody (M01), clone 2F2.Lane 1: TRIM24 transfected lysate(116.8 KDa).Lane 2: Non-transfected lysate.

Immunoprecipitation: TIF1 alpha Antibody (2F2) [H00008805-M01] - Analysis of TRIM24 transfected lysate using anti-TRIM24 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM24 MaxPab rabbit polyclonal antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
• Clone: 2F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TIF1 alpha Antibody (2F2) Summary

• Immunogen: TRIM24 (AAH28689, 432 a.a. ~ 569 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PQMPKQNPVVEQNSQPPSGLSSNQLSKFPTQISLAQLRLQHMQQQVMAQRQQVQRRPAPVGLPNPRMQGPIQQPSISHQQPPPRLINFQNHSPKPNGPVLPPHPQQLRYPPNQNIPRQAIKPNPLQMAFLAQQAIKQW
• Specificity: TRIM24 - tripartite motif-containing 24
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM24
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Proximity Ligation Assay
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TIF1 alpha Antibody (2F2)

• E3 ubiquitin-protein ligase TRIM24
• EC 6.3.2
• EC 6.3.2.-
• hTIF1
• PTC6
• RING finger protein 82
• RNF82
• RNF82Tif1a
• TIF1 alpha
• TIF1
• TIF1A
• TIF1-alpha
• TIF1ATIF1ALPHA
• TIF1TF1A
• transcription intermediary factor 1-alpha
• transcriptional intermediary factor 1
• TRIM24
• tripartite motif containing 24
• tripartite motif-containing 24
• Tripartite motif-containing protein 24

Background

The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors, including the estrogen, retinoic acid, and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains - a RING, a B-box type 1 and a B-box type 2 - and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIF1 alpha Antibody (H00008805-M01)(4)

Publications using H00008805-M01

• Dingar D, Kalkat M, Chan PK et al. BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors. J Prot. 2014-01-01 [PMID: 26217692]
• Dingar D, Kalkat M, Chan PK et al. BioID identifies novel c-MYC interacting partners in cultured cells and xenograft tumors. J Proteomics 2015-04-06 [PMID: 25452129]
• Liu X, Huang Y, Yang D et al. Overexpression of TRIM24 Is Associated with the Onset and Progress of Human Hepatocellular Carcinoma. PLoS One. 2014-01-07 [PMID: 24409330]
• Kikuchi M, Okumura F, Tsukiyama T et al. TRIM24 mediates ligand-dependent activation of androgen receptor and is repressed by a bromodomain-containing protein, BRD7, in prostate cancer cells. Biochim Biophys Acta. 2009-11-10 [PMID: 19909775]

Bioinformatics

• Gene Symbol: TRIM24
• Entrez:
  • 8805(Human)
• OMIM:
  • 188550(Human)
• Uniprot:
  • AAH28689(Human)