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SRC1 Antibody (1135)

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Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.
Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.
Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ChIP, IP, ICC/IF
Clone
1135
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NB200-310AF405
Alexa Fluor 488
NB200-310AF488
Alexa Fluor 647
NB200-310AF647
Alexa Fluor 700
NB200-310AF700
Biotin
NB200-310B
DyLight 350
NB200-310UV
DyLight 405
NB200-310V
DyLight 488
NB200-310G
DyLight 550
NB200-310R
DyLight 650
NB200-310C
DyLight 680
NB200-310FR
DyLight 755
NB200-310IR
FITC
NB200-310F
HRP
NB200-310H
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SRC1 Antibody (1135) Summary

Immunogen
Amino acids 477-947 of human SRC-1.
Specificity
This is specific for SRC-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCOA1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunoprecipitation 0.5-2 ug/ml
  • Immunofluorescence
Application Notes
IP and IF usages reported in scientific literature. WB: Predicted molecular weight: 169 kDa.
Control
Kidney Lysate (NB820-59231)
Reviewed Applications
Read 1 Review rated 4
using
NB200-310 in the following applications:
Publications
Read Publications using
NB200-310 in the following applications:

  • IP
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for SRC1 Antibody (1135)

  • bHLHe42
  • BHLHE74
  • Class E basic helix-loop-helix protein 74
  • EC 2.3.1.48
  • Hin-2 protein
  • KAT13A
  • MGC129719
  • NCoA-1
  • nuclear receptor coactivator 1
  • PAX3/NCOA1 fusion protein
  • Protein Hin-2
  • Renal carcinoma antigen NY-REN-52
  • RIP160bHLHe74F-SRC-1
  • SRC-1
  • SRC1MGC129720
  • Steroid receptor coactivator 1
  • steroid receptor coactivator-1

Background

SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160-kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of human breast adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:250. Detection : DAB
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Samples: FFPE sections of human breast adenocarcinoma (upper image) and prostate adenocarcinoma (lower image). Antibody: Affinity purified rabbit anti-SRC2 NB100-1756 used at a dilution of 1:100. Detection: DAB
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB
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Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.
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Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CBP/KAT3A Antibody [NB100-381] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-382. For blotting immunoprecipitated CBP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
(2 Reviews)
(1 Publication)
Species: Hu
Applications: WB, ChIP, IP
KAT3B/p300
Antibody (RW105)
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Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-KAT3B/p300 [RW105] at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Flow (Intracellular): KAT3B/p300 Antibody (RW105) [NB100-616] - An intracellular stain was performed on RAW 246.7 cells with KAT3B/p300 antibody (RW105) NB100-616PE (blue) and a matched isotype control NBP2-27287PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
(9 Publications)
Species: Hu, Mu, Rt, Md, Pm
Applications: WB, ChIP, EM, ICC/IF, IP, In vitro, Flow-IC
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Western Blot: MCG10 Antibody [NBP1-76729] - HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 u/mL.
Immunocytochemistry/Immunofluorescence: MCG10 Antibody [NBP1-76729] - HeLa cells with MCG10 antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: MCG10 Antibody [NBP1-76729] - Hela cells.
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Immunohistochemistry-Paraffin: PAG1 Antibody (PAG-C1) [NB500-487] - staining (paraffin sections) of PAG/Cbp in germinal center of lymphatic follicle and in dispersed T cells in appendix tissue by monoclonal antibody PAG-C1. Positive signal in T cells.
Immunoprecipitation: PAG1 Antibody (PAG-C1) [NB500-487] - Immunoprecipitation of human Cbp/PAG from the lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with anti-human Cbp/PAG (MEM-255). Note: Csk-binding protein (Cbp/PAG) is a 46 kDa adaptor protein, which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Lane 1,2: immunoprecipitation with anti-Cbp/PAG (PAG-C1) Lane 3: immunoprecipitation with anti-Cbp/PAG (polyclonal antibody).
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Applications: WB, IHC, IHC-P, IP
Opsin 1 (Long
Wave) RNAi
H00005956-R02
Species: Hu
eIF4E [p Ser209]
Antibody
NB100-81898
Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody.
Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) antibody
Immunohistochemistry: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E (phospho-Ser209) antibody
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Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
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Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).
(2 Publications)
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Western Blot: NCOR1 Antibody [NB100-58824] - Detection of Human and Mouse NCoR by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa and (50 ug) from mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibody NB100-58824 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.
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Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry: NCOR2 Antibody [NB120-5802] - Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Human lung tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).
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Immunohistochemistry: MYBBP1A Antibody [NB100-61050] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- MYBBP1a used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MYBBP1A Antibody [NB100-61050] - Detection of Human MYBBP1a by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-MYBBP1a antibody NB100-61050 used for IP at 3 mcg/mg lysate. MYBBP1a was also immunoprecipitated by rabbit anti-MYBBP1a antibody NB100-61051, which recognizes a downstream epitope.
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Immunocytochemistry/Immunofluorescence: SRA1 Antibody [NBP2-47260] - Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SRA1 Antibody [NBP2-47260] - Staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
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Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Immunohistochemistry: PELP1 Antibody [NB200-331] - Samples: FFPE sections of human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR ~Cat. No. Lot2 used at a dilution of 1:2,500 (0.4ug/ml) (left) and Cat. No. Lot3 used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.
Western Blot: PELP1 Antibody [NB200-331] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PELP1 antibody NB200-331 lot number 2 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). PELP1 was also immunoprecipitated by previous lot 1 of this antibody. Detection: Chemiluminescence with exposure times of 10 second (A) and 3 seconds (B).
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Western Blot: MSH6 Antibody [NB100-328] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-MSH6 antibody NB100-328 used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunohistochemistry: MSH6 Antibody [NB100-328] - Sample: FFPE section of human non-small cell lung cancer. Antibody: Affinity purified rabbit anti-MSH6 used at a dilution of 1:500 (2ug/ml). Detection: DAB
Western Blot: MSH6 Antibody [NB100-328] - Sample: Whole cell lysate (50 ug for WB; 1 mg for IP) from HeLa (A) and HCT116 3-6 (B) cells. Antibodies: Affinity purified goat anti-MSH6 used at 1 ug/ml for WB (A) and 1 ug/mg lysate for IP (B). For IP/WB (B), MSH6 was detected using a monoclonal antibody to MSH6. Detection: Chemiluminescence with 1 min exposure.
(2 Publications)
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Applications: IP (-), WB, IHC, IHC-P, IP
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(BuGR2)
NB300-731
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(2 Reviews)
(7 Publications)
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
SRC1 Antibody
(1135)
NB200-310
SRC1 Antibody (1135)
Species: Hu, Mu, Rt, Mk
Applications: WB, ChIP, IP, ICC/IF

Publications for SRC1 Antibody (NB200-310)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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Review for SRC1 Antibody (NB200-310) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB200-310:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot SRC1 NB200-310
Enlarge
reviewed by:
Anonymous
 WB Human 10/03/2014
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Summary

ApplicationWestern Blot
Sample TestedJurkat cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:500, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol

FAQs for SRC1 Antibody (NB200-310) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-310AF405
Alexa Fluor 488 Labeled NB200-310AF488
Alexa Fluor 647 Labeled NB200-310AF647
Alexa Fluor 700 Labeled NB200-310AF700
DyLight 350 Labeled NB200-310UV
DyLight 405 Labeled NB200-310V
DyLight 488 Labeled NB200-310G
DyLight 680 Labeled NB200-310FR
DyLight 755 Labeled NB200-310IR
Learn about Custom Antibody Labeling

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Blogs on SRC1

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Western Blot SRC1 NB200-310
Enlarge
 Anonymous
10/03/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol NCOA1
Entrez