SRC1 Antibody (1135)

Images

 
Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.
Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Sample (50 ug of whole cell lysate) A: Mouse brain 5% SDS PAGE, antibody diluted at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, MkSpecies Glossary
Applications WB, ChIP, IP, IF
Clone
1135
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

SRC1 Antibody (1135) Summary

Immunogen
Amino acids 477-947 of human SRC-1.
Specificity
This is specific for SRC-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCOA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunoprecipitation 0.5-2 ug/ml
  • Immunofluorescence
Application Notes
IP and IF usages reported in scientific literature. WB: Predicted molecular weight: 169 kDa.
Positive Control
Kidney Lysate (NB820-59231)
Reviewed Applications
Read 1 Review rated 4
using
NB200-310 in the following applications:

Publications
Read Publications using
NB200-310 in the following applications:

  • IP
    4 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for SRC1 Antibody (1135)

  • bHLHe42
  • BHLHE74
  • Class E basic helix-loop-helix protein 74
  • EC 2.3.1.48
  • Hin-2 protein
  • KAT13A
  • MGC129719
  • NCoA-1
  • nuclear receptor coactivator 1
  • PAX3/NCOA1 fusion protein
  • Protein Hin-2
  • Renal carcinoma antigen NY-REN-52
  • RIP160bHLHe74F-SRC-1
  • SRC-1
  • SRC1MGC129720
  • Steroid receptor coactivator 1
  • steroid receptor coactivator-1

Background

SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160-kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-310
Species: Hu, Mu, Rt, Mk
Applications: WB, ChIP, IP, IF

Publications for SRC1 Antibody (NB200-310)(4)

Review for SRC1 Antibody (NB200-310) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB200-310:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot SRC1 NB200-310
Enlarge
reviewed by:
Anonymous
WB Human 10/03/2014
View

Summary

ApplicationWestern Blot
Sample TestedJurkat cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:500, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SRC1 Antibody (NB200-310) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB200-310AF405
Alexa Fluor 488 Labeled NB200-310AF488
Alexa Fluor 647 Labeled NB200-310AF647
Alexa Fluor 700 Labeled NB200-310AF700
DyLight 350 Labeled NB200-310UV
DyLight 405 Labeled NB200-310V
DyLight 488 Labeled NB200-310G
DyLight 680 Labeled NB200-310FR
DyLight 755 Labeled NB200-310IR

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Bioinformatics Tool for SRC1 Antibody (NB200-310)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Anonymous
10/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol NCOA1
Entrez