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SRC1 Antibody (1135)

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 (1 Review)
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Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.
Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ChIP, IP
Clone
1135
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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SRC1 Antibody (1135) Summary

Immunogen
Amino acids 477-947 of human SRC-1.
Specificity
This is specific for SRC-1.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
NCOA1
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunoprecipitation 0.5-2 ug/ml
Application Notes
IP reactivity reported in (PMID: 19934375).
Theoretical MW
157 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Human Kidney Whole Tissue Lysate (Adult Whole Normal)
Reviewed Applications
Read 1 Review rated 4
using
NB200-310 in the following applications:
Publications
Read Publications using
NB200-310 in the following applications:

  • IP
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
No Preservative
Purity
Protein G purified

Alternate Names for SRC1 Antibody (1135)

  • bHLHe42
  • BHLHE74
  • Class E basic helix-loop-helix protein 74
  • EC 2.3.1.48
  • Hin-2 protein
  • KAT13A
  • MGC129719
  • NCoA-1
  • nuclear receptor coactivator 1
  • PAX3/NCOA1 fusion protein
  • Protein Hin-2
  • Renal carcinoma antigen NY-REN-52
  • RIP160bHLHe74F-SRC-1
  • SRC-1
  • SRC1MGC129720
  • Steroid receptor coactivator 1
  • steroid receptor coactivator-1

Background

SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160-kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative images taken from estradiol-treated wt mice. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB
Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative image taken from vehicle control wt mice Insets show the magnified image of an area within the small square box. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.
(1 Review)
(3 Publications)
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Applications: WB, ICC/IF, IHC, IHC-P
NCOA3/AIB1 Antibody
NB100-314
Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Detection of Human SRC3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SRC3 antibody NB100-314 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: NCOA3/AIB1 Antibody [NB100-314] - Nuclear extract (5 mcg for WB, 10 mg for CoIP) from HeLa cells. Affinity purified rabbit anti-SRC3 used at 0.33 mcg/ml for WB and 20 mcg/10 mg extract for CoIP.
(1 Review)
(4 Publications)
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NB100-381
Western Blot: CBP/KAT3A Antibody [NB100-381] - Detection of Human CBP by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody NB100-381 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: CBP/KAT3A Antibody [NB100-381] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-CBP antibody used for IP at 6 ug/mg lysate. CBP was also immunoprecipitated by rabbit anti-CBP antibody NB100-382. For blotting immunoprecipitated CBP, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
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(1 Publication)
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KAT3B/p300
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Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 min and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B Antibody (RW105)) at 5ug/ml for 60 minutes at room temperature and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective.
Western Blot: KAT3B/p300 Antibody (RW105) [NB100-616] - p300 detected in a HeLa nuclear extract using NB 100-616 (1:250). ECL: 20 minute exposure.
Immunocytochemistry/Immunofluorescence: KAT3B/p300 Antibody (RW105) [NB100-616] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-KAT3B/p300 Antibody (RW105) NB100-616 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
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Applications: WB, ChIP, EM, Flow, ICC/IF, IP, In vitro, Flow-IC
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Western Blot: MCG10 Antibody [NBP1-76729] - HeLa cell lysate with MCG10 antibody at (A) 0.5, (B) 1 and (C) 2 u/mL.
Immunocytochemistry/Immunofluorescence: MCG10 Antibody [NBP1-76729] - HeLa cells with MCG10 antibody at 1 ug/ml.
Immunocytochemistry/Immunofluorescence: MCG10 Antibody [NBP1-76729] - Hela cells.
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Western blot shows lysates of Daudi human Burkitt's lymphoma cell line and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAG1 Polyclonal Antibody (Catalog # MAB5285) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for PAG1 at approximately 70-85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and Daudi human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PAG1 at approximately 76 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human PAG1 Monoclonal Antibody (Catalog # MAB5285). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Applications: WB, Simple Western
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Western Blot: eIF4E [p Ser209] Antibody [NB100-81898] - Analysis of extracts from SK-BR-3 cells, untreated or insulin and EGF treated, and pretreated with U0126 cells, using eIF4E (Phospho-Ser209) Antibody.
Immunocytochemistry/Immunofluorescence: eIF4E [p Ser209] Antibody [NB100-81898] - Staining of methanol-fixed MCF cells using eIF4E(Phospho-Ser209) antibody
Immunohistochemistry: eIF4E [p Ser209] Antibody [NB100-81898] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E (phospho-Ser209) antibody
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Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
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NB300-560
Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).
(1 Review)
(7 Publications)
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Applications: WB, IHC, IHC-Fr, IHC-P, IF
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NB100-58824
Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
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H00005241-M08
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Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of U251 cells (right) compared to a negative control without primary antibody (left).
Immunohistochemistry: NCOR2 Antibody [NB120-5802] - Immunohistochemistry analysis of NCoR2 showing positive staining in the nucleus of paraffin-treated Human lung tissue (right) compared with a negative control in the absence of primary antibody (left).
Immunocytochemistry/Immunofluorescence: NCOR2 Antibody [NB120-5802] - Immunofluorescent analysis of NCoR2 (green) showing staining in the nucleus of Neuro-2a cells (right) compared to a negative control without primary antibody (left).
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP, ChIP
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Immunohistochemistry-Paraffin: MYBBP1A Antibody [NB100-61050] - Human colon carcinoma. Antibody: Affinity purified rabbit anti-MYBBP1A used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: MYBBP1A Antibody [NB100-61050] - Detection of human MYBBP1A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MYBBP1A antibody NB100-61050 used for IP at 3 ug per reaction. MYBBP1A was also immunoprecipitated by rabbit anti-MYBBP1A antibody NB100-61051. For blotting immunoprecipitated MYBBP1A, NB100-61051 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
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Applications: WB, IHC, IHC-P, IP, PLA
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Western Blot: SRA1 Antibody (1D4H8) [NBP1-51531] - Analysis using SRA mouse mAb against truncated SRA recombinant protein (1), human ovary cancer tissue lysate (2) and A431 cell lysate (3).
Immunohistochemistry-Paraffin: SRA1 Antibody (1D4H8) [NBP1-51531] - Analysis of human skin carcinoma (left) and breast carcinoma (right), showing cytoplasmic and membrane localization using SRA mouse mAb with DAB staining.
(1 Publication)
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Applications: WB, ELISA, IHC, IHC-P
PPAR gamma/NR1C3
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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
Immunohistochemistry-Paraffin: PPAR gamma Antibody [NBP2-22106] - IHC analysis of PPAR gamma in mouse liver.
Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
(2 Reviews)
(5 Publications)
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Western Blot: PELP1 Antibody [NB200-331] - Detection of mouse PELP1/MNAR by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: PELP1 Antibody [NB200-331] - Human ovarian carcinoma.~ Antibodies: Affinity purified rabbit anti-PELP1/MNAR used at a dilution of 1:2,500 (0.4ug/ml) (left) used at a dilution of 1:5,000 (0.2ug/ml) (right). Detection: DAB.
Western Blot: PELP1 Antibody [NB200-331] - Detection of Human PELP1/MNAR by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PELP1/MNAR antibody NB200-331 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
(18 Publications)
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Western Blot: MSH6 Antibody [NBP1-83319] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunocytochemistry/Immunofluorescence: MSH6 Antibody [NBP1-83319] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: MSH6 Antibody [NBP1-83319] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
GR/NR3C1 Antibody
(BuGR2)
NB300-731
Western Blot: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of glucocorticoid receptor on mouse liver extract.
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Monoclonal Antibody (BuGR2) shows staining in U251 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [NB300-731] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HeLa cells with GR/NR3C1 (BuGR2) antibody NB300-731AF647 (blue) and a matched isotype control NB600-986AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
(3 Reviews)
(11 Publications)
Species: Hu, Mu, Rt, Gp, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N

Publications for SRC1 Antibody (NB200-310)(4)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IP.


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Review for SRC1 Antibody (NB200-310) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB200-310:
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Images Ratings Applications Species Date Details
Western Blot SRC1 NB200-310
Enlarge 		4
reviewed by:
Anonymous
 WB Human 10/03/2014
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Summary

ApplicationWestern Blot
Sample TestedJurkat cell lysate
SpeciesHuman

Blocking

Blocking Details5% milk in TBST buffer

Primary Anitbody

Dilution Ratio1:500, diluted in TBST buffer+5% milk

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP (SCBT)
Secondary Manufacturer Cat#sc-2031
Secondary Concentration1:5,000

Details

Detection NotesDetected using Santa Cruz Western Blotting Luminol Reagent, Exposure 1 min

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SRC1 Antibody (NB200-310) (0)

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Control Lysate(s)

Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

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Bioinformatics Tool for SRC1 Antibody (NB200-310)

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Blogs on SRC1

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Western Blot SRC1 NB200-310
Enlarge
 Anonymous
10/03/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol NCOA1
Entrez