SRC1 Antibody (1135)

Western Blot: SRC1 Antibody (1135/H4) [NB200-310] - Mouse tissue (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with SRC1 antibody [1135] diluted at 1:2000.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB, ChIP, IP
• Clone: 1135
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

SRC1 Antibody (1135) Summary

• Immunogen: Amino acids 477-947 of human SRC-1.
• Specificity: This is specific for SRC-1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCOA1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Immunoprecipitation 0.5-2 ug/ml
  • Western Blot 1:500-1:3000
• Application Notes: IP reactivity reported in (PMID: 19934375).
• Theoretical MW: 157 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Human Kidney Whole Tissue Lysate (Adult Whole Normal)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for SRC1 Antibody (1135)

• bHLHe42
• bHLHe74
• Class E basic helix-loop-helix protein 74
• EC 2.3.1.48
• Hin-2 protein
• KAT13A
• MGC129719
• NCOA1
• NCoA-1
• nuclear receptor coactivator 1
• PAX3/NCOA1 fusion protein
• Protein Hin-2
• Renal carcinoma antigen NY-REN-52
• RIP160
• RIP160bHLHe74F-SRC-1
• SRC1
• SRC-1
• SRC1MGC129720
• Steroid receptor coactivator 1
• steroid receptor coactivator-1

Background

SRC-1 is a member of a gene family that functions in the full transcriptional activation of the steroid hormone receptor superfamily and contains intrinsic histone acetyltransferase activity. SRC-1 has a molecular weight of 160-kDa and has a basic helix-loop-helix (bHLH)-PAS domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SRC1 Antibody (NB200-310)(4)

Publications using NB200-310

• Amita M, Takahashi T, Tsutsumi S et al. Molecular mechanism of the inhibition of estradiol-induced endometrial epithelial cell proliferation by clomiphene citrate. Endocrinology 2010-01-01 [PMID: 19934375] (IP, Human)
• Jaber BM, Gao T, Huang L et al. The pure estrogen receptor antagonist ICI 182,780 promotes a novel interaction of estrogen receptor-alpha with the 3',5'-cyclic adenosine monophosphate response element-binding protein-binding protein/p300 coactivators. Mol Endocrinol 2006-11-01 [PMID: 16840538] (IP, Human)
• Klinge CM, Jernigan SC, Mattingly KA et al. Estrogen response element-dependent regulation of transcriptional activation of estrogen receptors alpha and beta by coactivators and corepressors. J Mol Endocrinol 2004-10-01 [PMID: 15525597] (IP, Human)
• Dutertre M, Smith CL. Ligand-independent interactions of p160/steroid receptor coactivators and CREB-binding protein (CBP) with estrogen receptor-alpha: regulation by phosphorylation sites in the A/B region depends on other receptor domains. Mol Endocrinol 2003-07-01 [PMID: 12714702] (IP, Human)

Review for SRC1 Antibody (NB200-310) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Costache Vasilescu WB Human 10/03/2014

Summary

• Application: Western Blot
• Sample Tested: Jurkat cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: NCOA1
• Entrez:
  • 20779(Mouse)
  • 83805(Rat)