Tie-1 Antibody (88016)

Images

 
Tie‑1 was detected in immersion fixed paraffin-embedded sections of human gastric cancer tissue using Mouse Anti-Human Tie‑1 Monoclonal Antibody (Catalog # MAB619) at 25 µg/mL overnight at 4 °C. Tissue was stained ...read more
Tie‑1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Tie‑1 Monoclonal Antibody (Catalog # MAB619) at 10 µg/mL for 3 hours at room temperature. Cells were stained ...read more
Tie‑1 was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human Tie‑1 Monoclonal Antibody (Catalog # MAB619) at 15 µg/mL overnight at 4 °C. Tissue was stained using ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, ICC/IF
Clone
88016
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

Tie-1 Antibody (88016) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Tie-1
Ala22-Gln760 (predicted)
Accession # P35590
Specificity
Detects human Tie-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TIE1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
MAB619 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-1 Antibody (88016)

  • EC 2.7.10
  • EC 2.7.10.1
  • JTK14soluble TIE1 variant 3
  • receptor tyrosine kinase
  • soluble TIE1 variant 1
  • soluble TIE1 variant 2
  • soluble TIE1 variant 5
  • Tie1
  • Tie-1
  • TIEsoluble TIE1 variant 4
  • tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1
  • tyrosine kinase with immunoglobulin-like and EGF-like domains 1
  • tyrosine-protein kinase receptor Tie-1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-1 cDNA encodes a 1138 amino acid (aa) precursor with a 24 aa putative signal peptide, a 735 aa extracellular domain and a 354 aa cytoplasmic domain. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

  1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
  2. Sato, T.N. et al. (1995) Nature 376:70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Tie-1 Antibody (MAB619)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC, Western Blot.


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ICC
(1)
Western Blot
(1)
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(2)
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Bioinformatics

Gene Symbol TIE1
Uniprot