Tie-1 Antibody (88016)

Tie-1 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Tie-1 was detected in immersion fixed paraffin-embedded sections of human gastric cancer tissue using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in the pyloric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Tie-1 was detected in immersion fixed paraffin-embedded sections of human stomach array using Mouse Anti-Human Tie-1 Monoclonal Antibody (Catalog # MAB619) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC, ICC/IF
• Clone: 88016
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Tie-1 Antibody (88016) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Tie-1
  Ala22-Gln760 (predicted)
  Accession # P35590
• Specificity: Detects human Tie-1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIE1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Tie-1 Antibody (88016)

• EC 2.7.10
• EC 2.7.10.1
• JTK14soluble TIE1 variant 3
• receptor tyrosine kinase
• soluble TIE1 variant 1
• soluble TIE1 variant 2
• soluble TIE1 variant 5
• Tie1
• Tie-1
• TIEsoluble TIE1 variant 4
• tyrosine kinase with immunoglobulin and epidermal growth factor homologydomains 1
• tyrosine kinase with immunoglobulin-like and EGF-like domains 1
• tyrosine-protein kinase receptor Tie-1

Background

Tie-1/Tie (tyrosine kinase with Ig and EGF homology domains 1) and Tie-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human Tie-1 cDNA encodes a 1138 amino acid (aa) precursor with a 24 aa putative signal peptide, a 735 aa extracellular domain and a 354 aa cytoplasmic domain. Based on gene-targeting studies, the in vivo functions of Tie-1 have been shown to be related to endothelial cell differentiation and the maintenance of integrity of the endothelium.

1. Partanen, J. and D.J. Dumont (1999) Curr. Top. Microbiol. Immunol. 237:159.
2. Sato, T.N. et al. (1995) Nature 376:70.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Tie-1 Antibody (MAB619)(4)

Publications using MAB619

• ME Griffin, AW Sorum, GM Miller, WA Goddard, LC Hsieh-Wils Sulfated glycans engage the Ang-Tie pathway to regulate vascular development Nat Chem Biol, 2020-10-05;0(0):. 2020-10-05 [PMID: 33020664] (ICC, Human)
• Ramazan Demir Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy Folia Histochemica et Cytobiologica 2/19/2010 [PMID: 20164029]
• Ramazan Demir Expression of VEGF receptors VEFGR-1 and VEGFR-2, angiopoietin receptors Tie-1 and Tie-2 in chorionic villi tree during early pregnancy Folia Histochemica et Cytobiologica 2010-02-19 [PMID: 20164029] (Immunohistochemistry, Human)
• Kayisli UA, Cayli S, Seval Y, Tertemiz F, Huppertz B, Demir R Spatial and temporal distribution of Tie-1 and Tie-2 during very early development of the human placenta. Placenta, 2005-07-18;27(6):648-59. 2005-07-18 [PMID: 16026828] (IHC-P, Western Blot, Human)

Bioinformatics

• Gene Symbol: TIE1
• Uniprot:
  • P35590()