THAP11 Antibody

Western Blot: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in HeLa whole cell extracts.

Immunocytochemistry/Immunofluorescence: THAP11 Antibody [NBP1-49463] - Immunocytochemical analysis of THAP11 in HeLa cells.

Immunohistochemistry: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in mouse skin

Western Blot: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in HepG2 whole cell lysates.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

THAP11 Antibody Summary

• Immunogen: Partial recombinant human THAP11 protein expressed in E. coli [Swiss-Prot# Q96EK4]
• Localization: Nucleus. Cytoplasm Note: May be regulated by shuttling of the protein between the cytoplasm and nucleus.
• Predicted Species: Rat (94%), Bovine (95%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: THAP11
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
  • Western Blot 1:10000
• Application Notes: This THAP11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~26 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  HeLa Whole Cell Lysate
  HepG2 Whole Cell Lysate

Reactivity Notes

Human and mouse. Immunogen sequence has 94% identity to rat and 95% identity to bovine.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for THAP11 Antibody

• CTG-B43a
• CTG-B45d
• HRIHFB2206
• Ronin
• THAP domain containing 11
• THAP domain-containing protein 11
• THAP11

Background

Thanatos-associated protein (THAP) domain is an evolutionarily conserved C2-CH (C-X2-4-C-X35-50-C-X2-H) zinc finger motif which has sequence-specific DNA-binding activity and twelve THAP proteins, each containing an N-terminally located THAP domain, have been identified in humans (THAP0 to THAP11), some of which (THAP0, -1, -2, -4, -7, and -11) are also conserved in mice/rats. Individual THAP proteins have been implicated in a diverse array of physiological processes, including cell proliferation, regulation of transcription, apoptosis, and maintenance of embryonic stem (ES) cell pluripotency. THAP11 is regulated by shuttling between cytoplasm - nucleus, and is considered a transcriptional repressor that plays a central role in embryogenesis as well as in ES cell pluripotency. It act as a sequence-specific DNA-binding factor which represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. THAP11 has been suggested as a candidate for polyglutamine disorders based on polymorphism and protein-folding simulation studies. RONIN is the mouse homolog of THAP11 and its homozygous deletion happened to be embryonically lethal in mice wherein was shown to be essential factor underlying embryogenesis/ES cell pluripotency via its activational/ repressional effects on transcription. THAP11 also functions as a negative regulator of cell growth in human HepG2 hepatoma cells through transcriptional repression of the proto-oncogene MYC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THAP11
• Entrez:
  • 57215(Human)
• Uniprot:
  • Q96EK4()