THAP11 Antibody

Images

 
Western Blot: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in HeLa whole cell extracts.
Immunocytochemistry/ Immunofluorescence: THAP11 Antibody [NBP1-49463] - Immunocytochemical analysis of THAP11 in HeLa cells.
Immunohistochemistry: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in mouse skin
Western Blot: THAP11 Antibody [NBP1-49463] - Analysis of THAP11 in HepG2 whole cell lysates.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

THAP11 Antibody Summary

Immunogen
Partial recombinant human THAP11 protein expressed in E. coli [Swiss-Prot# Q96EK4]
Localization
Nucleus. Cytoplasm Note: May be regulated by shuttling of the protein between the cytoplasm and nucleus.
Predicted Species
Rat (94%), Bovine (95%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
THAP11
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Immunocytochemistry/Immunofluorescence 1:1000
  • Immunohistochemistry 1:300
  • Immunohistochemistry-Paraffin 1:300
Application Notes
This THAP11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western blot, where a band is seen at ~26 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)
HepG2 Lysate (NBP1-42569)

Reactivity Notes

Human and mouse. Immunogen sequence has 94% identity to rat and 95% identity to bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for THAP11 Antibody

  • CTG-B43a
  • CTG-B45d
  • HRIHFB2206
  • Ronin
  • THAP domain containing 11
  • THAP domain-containing protein 11
  • THAP11

Background

Thanatos-associated protein (THAP) domain is an evolutionarily conserved C2-CH (C-X2-4-C-X35-50-C-X2-H) zinc finger motif which has sequence-specific DNA-binding activity and twelve THAP proteins, each containing an N-terminally located THAP domain, have been identified in humans (THAP0 to THAP11), some of which (THAP0, -1, -2, -4, -7, and -11) are also conserved in mice/rats. Individual THAP proteins have been implicated in a diverse array of physiological processes, including cell proliferation, regulation of transcription, apoptosis, and maintenance of embryonic stem (ES) cell pluripotency. THAP11 is regulated by shuttling between cytoplasm - nucleus, and is considered a transcriptional repressor that plays a central role in embryogenesis as well as in ES cell pluripotency. It act as a sequence-specific DNA-binding factor which represses gene expression in pluripotent ES cells by directly binding to key genetic loci and recruiting epigenetic modifiers. THAP11 has been suggested as a candidate for polyglutamine disorders based on polymorphism and protein-folding simulation studies. RONIN is the mouse homolog of THAP11 and its homozygous deletion happened to be embryonically lethal in mice wherein was shown to be essential factor underlying embryogenesis/ES cell pluripotency via its activational/ repressional effects on transcription. THAP11 also functions as a negative regulator of cell growth in human HepG2 hepatoma cells through transcriptional repression of the proto-oncogene MYC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

View specific protocols for THAP11 Antibody (NBP1-49463): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol THAP11
Entrez
Uniprot