Independent Antibodies: Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity ...read more
The immunogen recognized by this antibody maps to a region between residue 1700 and 1750 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Immunogen affinity purified
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Alternate Names for Host Cell Factor 1/HCFC1 Antibody
HCF-1VP16 accessory protein
host cell factor C1 (VP16-accessory protein)
VCAFhost cell factor 1
This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Host Cell Factor 1/HCFC1 Antibody (NB100-68209). (Showing 1 - 1 of 1 FAQs).
Hi, I'm trying to use your HCFC1 antibody for a western blot. I calculated the predicted size of the bands based on the cleavage sites in the protein and I'm getting a band that's similar to the one listed on your website, but 30 kd larger than what's calculated. I would appreciate any help clarifying this.
HCFC1 is subject to a number of post-translational modifications that would cause the band to migrate differently than the expected molecular weight. Our image for this antibody shows a number of bands to reflect the different fragments produced by proteolytic cleavage of the protein.
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