Host Cell Factor 1/HCFC1 Antibody

Images

 
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-HCF1 ...read more
Proximity Ligation Assay: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human MEN1 (NB100-287) and rabbit anti-human HCF1 (NB100-68209). Image merged from ...read more
Proximity Ligation Assay: Host Cell Factor 1/HCFC1 Antibody [NB100-68209] - Secondary-conjugate Duolink II PLA in Hela cells. goat anti-human SDS3 (NB100-257) and rabbit anti-human HCF1 (NB100-68209). Image merged from ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

       

Order Details

Host Cell Factor 1/HCFC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1700 and 1750 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HCFC1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 mcg/mg lysate
  • Proximity Ligation Assay 1:200-1:4000
Publications
Read Publications using
NB100-68209 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Host Cell Factor 1/HCFC1 Antibody

  • C1 Factor
  • CFF
  • CFFC1 factor
  • HCF
  • HCF1
  • HCF1HFC1
  • HCF-1VP16 accessory protein
  • HCFC1
  • HFC1
  • host cell factor C1 (VP16-accessory protein)
  • MGC70925
  • VCAFhost cell factor 1

Background

This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-21584
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
H00005452-D01P
Species: Hu, Mu, Rt
Applications: WB
NBP2-37576
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC
H00010488-M01
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-45374
Species: Hu
Applications: WB, IHC
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NB100-56530
Species: Hu
Applications: WB
MAB5526
Species: Hu, Mu
Applications: WB
NBP1-89544
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-233
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP, PLA
NBP2-45733
Species: Hu
Applications: WB, IHC, IF
MAB6799
Species: Hu
Applications: WB, IHC
NBP2-01223
Species: Hu
Applications: WB, Flow, IHC, IHC-P
NB100-1015
Species: Hu
Applications: WB, IHC, IHC-P, PEP-ELISA
NBP1-92273
Species: Hu
Applications: IHC, IHC-P
NBP2-36776
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IHC-P, IP, PA
NB100-68209
Species: Hu, Mu
Applications: WB, IP, PLA

Publications for Host Cell Factor 1/HCFC1 Antibody (NB100-68209)(3)

Reviews for Host Cell Factor 1/HCFC1 Antibody (NB100-68209) (0)

There are no reviews for Host Cell Factor 1/HCFC1 Antibody (NB100-68209). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Host Cell Factor 1/HCFC1 Antibody (NB100-68209). (Showing 1 - 1 of 1 FAQs).

  1. Hi, I'm trying to use your HCFC1 antibody for a western blot. I calculated the predicted size of the bands based on the cleavage sites in the protein and I'm getting a band that's similar to the one listed on your website, but 30 kd larger than what's calculated. I would appreciate any help clarifying this.
    • HCFC1 is subject to a number of post-translational modifications that would cause the band to migrate differently than the expected molecular weight. Our image for this antibody shows a number of bands to reflect the different fragments produced by proteolytic cleavage of the protein.

Secondary Antibodies

 

Isotype Controls

Additional Host Cell Factor 1/HCFC1 Products

Bioinformatics Tool for Host Cell Factor 1/HCFC1 Antibody (NB100-68209)

Discover related pathways, diseases and genes to Host Cell Factor 1/HCFC1 Antibody (NB100-68209). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Host Cell Factor 1/HCFC1 Antibody (NB100-68209)

Discover more about diseases related to Host Cell Factor 1/HCFC1 Antibody (NB100-68209).
 

Pathways for Host Cell Factor 1/HCFC1 Antibody (NB100-68209)

View related products by pathway.

PTMs for Host Cell Factor 1/HCFC1 Antibody (NB100-68209)

Learn more about PTMs related to Host Cell Factor 1/HCFC1 Antibody (NB100-68209).

Blogs on Host Cell Factor 1/HCFC1

There are no specific blogs for Host Cell Factor 1/HCFC1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Host Cell Factor 1/HCFC1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol HCFC1
Entrez
Uniprot