TCF7L1/TCF3 Antibody

Western blot shows lysates of human pancreas and human lung tissue. PVDF Membrane was probed with 0.2 µg/mL of Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TCF7L1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

TCF7L1 was detected in immersion fixed BG01V human embryonic stem cells using Human TCF7L1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6116) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Sheep IgG Secondary Antibody (green, upper panel; Catalog # NL012) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

TCF7L1/TCF3 Antibody Summary

• Immunogen: E. coli-derived recombinant human TCF7L1
  Lys429-Ser581
  Accession # Q9HCS4
• Specificity: Detects human TCF7L1 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: TCF7L1
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TCF7L1/TCF3 Antibody

• HMG box transcription factor 3
• TCF3
• TCF-3
• TCF7L1
• transcription factor 7-like 1 (T-cell specific, HMG-box)
• transcription factor 7-like 1

Background

Transcription factor 7-like 1 (TCF7L1; also HMG box transcription factor 3, TCF-3) is a 63 kDa member of the TCF/LEF family of proteins. Human TCF7L1 is 588 amino acids (aa) in length and contains one HMG box DNA-binding domain (aa 346-414). Human TCF7L1 shares 95% aa sequence identity with mouse TCF7L1. TCF7L1 is expressed in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium. Functionally, it participates in the Wnt signaling pathway. TCF7L1 binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. TCF7L1 is also necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TCF7L1/TCF3 Antibody (AF6116)(1)

Publication using AF6116

• S Mukherjee, DM Luedeke, L McCoy, M Iwafuchi, AM Zorn SOX transcription factors direct TCF-independent WNT/beta-catenin responsive transcription to govern cell fate in human pluripotent stem cells Cell Reports, 2022-08-23;40(8):111247. 2022-08-23 [PMID: 36001974] (IF, WB, Human)

Bioinformatics

• Gene Symbol: TCF7L1
• Uniprot:
  • Q9HCS4(Human)