Ornithine Decarboxylase Antibody (ODC1/485)

Images

 
Western Blot: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Analysis using the Azide and BSA Free version of NBP2-32887. Detection of ODC1 in human placental lysate
Immunocytochemistry/ Immunofluorescence: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - IF staining of LNCap cells using AF488 labeled ODC1 Monoclonal Antibody (ODC1/485) (GREEN) DAPI was used to stain the ...read more
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with Ornithine Decarboxylase Antibody (ODC1/485).
Flow Cytometry: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Flow Cytometric Analysis of human ODC-1 in PC3 cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled Ornithine Decarboxylase ...read more
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human prostate carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded human Testicular carcinoma with ODC-1 Monoclonal Antibody (ODC1/485)
Immunohistochemistry-Paraffin: Ornithine Decarboxylase Antibody (ODC1/485) [NBP2-32887] - Formalin-fixed, paraffin-embedded Rat pancreas stained with ODC1 Monoclonal Antibody (ODC1/485)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
ODC1/485
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Ornithine Decarboxylase Antibody (ODC1/485) Summary

Immunogen
Recombinant human Ornithine Decarboxylase protein
Localization
Cytoplasmic
Specificity
Recognizes a 53kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ODC1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-32887 in the following applications:

Publications
Read Publication using NBP2-32887.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Notes

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml (NBP2-34700).

Alternate Names for Ornithine Decarboxylase Antibody (ODC1/485)

  • ODC
  • ODC1
  • ODCEC 4.1.1.17
  • ornithine decarboxylase 1
  • ornithine decarboxylase

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ornithine Decarboxylase Antibody (NBP2-32887)(1)

We have publications tested in 1 application: WB.


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Review for Ornithine Decarboxylase Antibody (NBP2-32887) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-32887:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot Ornithine Decarboxylase NBP2-32887
Enlarge
5
reviewed by:
Andrea Flynn
WB Human 07/06/2017
View

Summary

ApplicationWestern Blot
Sample TestedHuman Neuroblastoma whole cell lysate
SpeciesHuman

Comments

CommentsUsed at 1:500 dilution overnight at 4C

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Andrea Flynn
07/06/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol ODC1
Uniprot