Recombinant TGF-beta 3 Protein

Images

 
Western Blot: TGF beta 3 Protein [NBP1-71646] - Analysis of recombinant TGF beta 3. Lane MWM: Molecular weight marker (kDa), lane 1: 0.2 ug of TGF beta 3.
SDS-Page: TGF beta 3 Protein [NBP1-71646] - Samples were loaded in 15% SDS-PAGE gel, stained with Coomassie blue. Lane MWM: (kDa); lane a: contains 0.2 ug of recombinant TGF B3. (reducing condition, ~13kDa monomer and ...read more
Functional: TGF beta 3 Protein [NBP1-71646]

Product Details

Summary
Product Discontinued
View other related TGF-beta 3 Peptides and Proteins

Order Details


    • Catalog Number
      NBP1-71646
    • Availability
      Product Discontinued

    Can't find what you are looking for? Use our Antibody Concierge Service & we will help you locate your antibody!

    Or feel free to contact us for alternative products.

Recombinant TGF-beta 3 Protein Summary

Description
A biologically reactive recombinant protein of Human TGF-beta 3.

Source: Nicotiana benthamiana

Amino Acid Sequence: HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS

Epitope
HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
Protein/Peptide Type
Biologically Active Protein
Gene
TGFB3

Applications/Dilutions

Application Notes
Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF beta 3. Biological Activity: The biological activity of TGF-beta 3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 less than 40ng/ml

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Tris HCl 0.05M buffer at pH 7.4.
Concentration
LYOPH
Reconstitution Instructions
Reconstitute with water to a final concentration of 50 ng/ul. Due to the protein nature, dimmers and multimers may be observed.

Notes

Molecular formula: C600 H902 N166 O174 S10 Extinction coefficient: E 0.1% 1.72 (A 280 nm) Molecular weight: recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond p.I: 6.75 Purity: > 97 % as determined by SDS-PAGE gel Endotoxin level* < 0.04 EU/ ìg protein (LAL method). Storage and Stability: It is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.

Alternate Names for Recombinant TGF-beta 3 Protein

  • ARVD
  • FLJ16571
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

Description: Recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. Source: It is produced by transient expression of TGF-beta 3 in non-transgenic plants. Recombinant human TGF-beta 3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product contains no animal derived components or impurities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3025
Species: Hu
Applications: WB, ICC
AF3797
Species: Hu, Mu
Applications: WB, Simple Western, ChIP, ICC
AF-241-NA
Species: Hu
Applications: WB, Flow, IHC, CyTOF-ready, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
NBP1-86078
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-74350
Species: Hu, Mu, Rt, Po, Bv, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP1-91258
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-56479
Species: Hu, Mu, Rt, Po, Bv, Ca, Pm
Applications: WB, IHC-P
AF947
Species: Hu
Applications: WB, Simple Western

Publications for TGF-beta 3 Protein (NBP1-71646) (0)

There are no publications for TGF-beta 3 Protein (NBP1-71646).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TGF-beta 3 Protein (NBP1-71646) (0)

There are no reviews for TGF-beta 3 Protein (NBP1-71646). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TGF-beta 3 Protein (NBP1-71646) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Recombinant TGF-beta 3 Protein Products

Related Products by Gene

Bioinformatics Tool for TGF-beta 3 Protein (NBP1-71646)

Discover related pathways, diseases and genes to TGF-beta 3 Protein (NBP1-71646). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TGF-beta 3 Protein (NBP1-71646)

Discover more about diseases related to TGF-beta 3 Protein (NBP1-71646).
 

Pathways for TGF-beta 3 Protein (NBP1-71646)

View related products by pathway.

PTMs for TGF-beta 3 Protein (NBP1-71646)

Learn more about PTMs related to TGF-beta 3 Protein (NBP1-71646).
 

Research Areas for TGF-beta 3 Protein (NBP1-71646)

Find related products by research area.

Blogs on TGF-beta 3

There are no specific blogs for TGF-beta 3, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Recombinant TGF-beta 3 Protein and receive a gift card or discount.

Bioinformatics

Gene Symbol TGFB3
Entrez
Uniprot