TGF-beta 3 Biologically Active Protein

Images

 
Western Blot: TGF beta 3 Protein [NBP1-71646] - Analysis of recombinant TGF beta 3. Lane MWM: Molecular weight marker (kDa), lane 1: 0.2 ug of TGF beta 3.
SDS-Page: TGF beta 3 Protein [NBP1-71646] - Samples were loaded in 15% SDS-PAGE gel, stained with Coomassie blue. Lane MWM: (kDa); lane a: contains 0.2 ug of recombinant TGF B3. (reducing condition, ~13kDa monomer and ...read more
Functional: TGF beta 3 Protein [NBP1-71646]

Product Details

Summary
Product Discontinued
View other related TGF-beta 3 Peptides and Proteins

Order Details


    • Catalog Number
      NBP1-71646
    • Availability
      Product Discontinued

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TGF-beta 3 Biologically Active Protein Summary

Description
A biologically reactive recombinant protein of Human TGF-beta 3.

Source: Nicotiana benthamiana

Amino Acid Sequence: HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS

Epitope
HHHHHHALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS
Gene
TGFB3

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
Tris HCl 0.05M buffer at pH 7.4.
Concentration
Lyoph
Reconstitution Instructions
Reconstitute with water to a final concentration of 50 ng/ul. Due to the protein nature, dimmers and multimers may be observed.

Applications/Dilutions

Application Notes
Serological Identification: The protein was electrophoresed under reducing condition on a 15% SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF beta 3. Biological Activity: The biological activity of TGF-beta 3 is measured in culture by its ability to inhibit the mink lung epithelial (Mv1Lu) cells proliferation. ED50 less than 40ng/ml

Notes

Molecular formula: C600 H902 N166 O174 S10 Extinction coefficient: E 0.1% 1.72 (A 280 nm) Molecular weight: recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid polypeptide chains linked by a single disulfide bond p.I: 6.75 Purity: > 97 % as determined by SDS-PAGE gel Endotoxin level* < 0.04 EU/ ìg protein (LAL method). Storage and Stability: It is recommended to add a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing is not recommended.

Alternate Names for TGF-beta 3 Biologically Active Protein

  • ARVD
  • FLJ16571
  • TGFB3
  • TGFbeta 3
  • TGF-beta 3
  • TGF-beta3
  • TGF-beta-3
  • transforming growth factor beta-3
  • transforming growth factor, beta 3

Background

Description: Recombinant human TGF-beta 3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulfide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta 1, TGF-beta 2 and TGF-beta 3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing. Source: It is produced by transient expression of TGF-beta 3 in non-transgenic plants. Recombinant human TGF-beta 3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC). This product contains no animal derived components or impurities.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol TGFB3
Entrez
Uniprot

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