Teneurin-1 Antibody - BSA Free

Western Blot: Teneurin-1 Antibody [NBP2-41315] - Western blot analysis of TENM1 in human brain tissue lysate with TENM1 antibody at 1 ug/ml.

Immunoprecipitation: Teneurin-1 Antibody - BSA Free [NBP2-41315] - HINT1 exhibits isopeptidase activity. (A) HINT1 interactions with the proteins used in the study. (I) HINT1 but not its paralogs forms stable complexes with RGSZ2 proteins. (II) ICD teneurin1 interacts with HINT1 but not with GST. (III, IV) HINT1 associations with RanGAP1 & SP100 were weak or not detected. (B) HINT1 exhibits SUMO protease activity on sumoylated RanGAP1, which is regulated by calcium & CaM. (I) HINT1 (2 μM) but not SENP2 (0.3 μM) requires calcium-activated CaM (6 μM) to exhibit sumoylase activity. (II) Removal of 10 mM MgCl2 from the sumoylation buffer greatly improves Ca2+-CaM-dependent HINT1 isopeptidase activity on sumoylated RanGAP1. (III, IV) HINT1 exhibits isopeptidase activity on sumoylated RanGAP1 in the presence of increasing concentrations of CaCl2 & of CaM. Details of immunoblot detection in “Materials & Methods” section & Supplementary Figures S5 & S6. GST, glutathione S-transferase; ICD, intracellular domain; RanGAP1, Ran GTPase-activating protein 1; RGSZ2, regulator of G protein signaling 17 (Z2); SENP, sentrin-specific protease. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31088288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunofluorescence of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 20 u/mL.

Immunohistochemistry: Teneurin-1 Antibody - BSA Free [NBP2-41315] - Immunohistochemistry of Teneurin-1 in mouse brain tissue with Teneurin-1 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Teneurin-1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Teneurin-1 Antibody - BSA Free (NBP2-41315) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Teneurin-1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against an 18 amino acid peptide near the amino terminus of human TENM1. The immunogen is located within the first 50 amino acids of TENM1. Amino Acid Squence: SDESEDGRKPRQSFNSRE
• Specificity: TENM1 antibody is predicted to not cross-react with other members of the TENM family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TENM1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 5 ug/mL
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1 - 2 ug/mL
• Theoretical MW: 280 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken: (83%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Teneurin-1 Antibody - BSA Free

• odz, odd Oz/ten-m homolog 1(Drosophila)
• ODZ1
• ODZ3
• Protein Odd Oz/ten-m homolog 1
• Ten-1
• tenascin M
• tenascin M1
• Tenascin-M1
• teneurin 1
• Teneurin1
• Teneurin-1
• TEN-M1
• TNM
• TNM1

Background

Teneurin-1, also known as Teneurin Transmembrane Protein 1 or Ten-1, contains a 305 kDa and a 306 kDa isoform, and is involved in regulating the connections within the nervous system and neural development. Teneurin-1 is not currently being used in any disease research. The protein has not been found to interact with any other proteins, but is linked to transcription, stress and immune responses, and signal transduction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Teneurin-1 Antibody (NBP2-41315)(1)

Publication using NBP2-41315

• Cortes-Montero E, Rodriguez-Munoz M, Sanchez-Blazquez P, and Garzon J. The axonal motor neuropathy-related HINT1 protein is a zinc- and calmodulin-regulated cysteine SUMO protease Antioxid. Redox Signal. 2019-05-15 [PMID: 31088288] (WB, Human)

Bioinformatics

• Gene Symbol: TENM1