EMMPRIN/CD147 Antibody [Unconjugated]

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Western blot shows lysates of human brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) followed by HRP-conjugated ...read more
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human stomach cancer tissue using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 5 µg/mL ...read more
EMMPRIN/CD147 was detected in immersion fixed paraffin-embedded sections of human cervix using Goat Anti-Human EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF972) at 1 µg/mL overnight at 4 ...read more
Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 70 kDa (as indicated) using 10 µg/mL of Goat ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and EMMPRIN/CD147 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat ...read more
Recombinant Human EMMPRIN/CD147 protein was serially diluted 2-fold and captured by Mouse Anti-Human EMMPRIN/CD147 Monoclonal Antibody (MAB972) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ELISA, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Conjugate Size Price
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EMMPRIN/CD147 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EMMPRIN/CD147
Thr25-His205
Accession # Q54A51
Specificity
Detects human EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse EMMPRIN is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
BSG
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF972 in the following applications:

Publications
Read Publications using
AF972 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMMPRIN/CD147 Antibody [Unconjugated]

  • 5F7
  • basigin (Ok blood group)
  • Basigin
  • BSG
  • CD147 antigen
  • CD147
  • Collagenase stimulatory factor
  • EMMPRIN
  • EMMPRINTCSF
  • Extracellular matrix metalloproteinase inducer
  • Leukocyte activation antigen M6
  • M6
  • OK blood group antigen
  • OK
  • Tumor cell-derived collagenase stimulatory factor

Background

Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 44-66 kDa, variably glycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily (1-4). Human EMMPRIN is 269 amino acids (aa) in length and contains a 24 aa signal sequence, a 183 aa extracellular domain (ECD), a 21 aa transmembrane (TM) domain and a 41 aa intracellular domain. The ECD contains one C2-type and one V-type Ig-like domain. There is one 385 aa splice variant that contains an extra N-terminal IgCAM domain and is found only in the retina (5). mRNA transcripts, but not protein, have been reported for additional 432, 388, 205, 176, and 174 aa variants.

EMMPRIN is expressed in areas of tissue remodeling, including tumors, endometrium, placenta, skin, and regions undergoing angiogenesis (1, 2, 6-9). It is also expressed in cells with high metabolic activity, such as lymphoblasts, macrophages and tumor cells (2, 10). On cells with elevated metabolic rates, EMMPRIN is often co-expressed with the amino acid transporter CD98h (11). EMMPRIN also interacts with caveolin-1 (via its C2-like domain), and this reduces the level of EMMPRIN glycosylation and subsequent EMMPRIN multimerization and activity (12). EMMPRIN’s TM sequence contains a Glu and a Pro which are important for intracellular interactions with cyclophilins (CyP) (3, 13, 14). CyPA (cyclosporin A receptor) and CyP60 interactions with the TM segment promote leukocyte inflammatory chemotaxis and surface expression of EMMPRIN, respectively (13, 14). An active 22 kDa fragment can be shed from tumor cells by MT1-MMP (1). Tumor cells can also release active, full-length EMMPRIN in microvesicles (15, 16). Functionally, EMMPRIN is known to induce urokinase-type plasminogen activator (uPA), VEGF, hyaluronan, and multiple MMPs (1, 2, 7, 8, 9). Human EMMPRIN (269 aa) shows 58%, 58%, 62%, and 52% aa identity with mouse, rat, bovine, and chicken EMMPRIN, respectively. It also shows 25% and 38% aa identity with the related proteins, embigin and neuroplastin (SDR-1), respectively (4).

  1. Gabison, E.E. et al. (2005) Biochimie 87:361.
  2. Yurchenko, V. et al. (2006) Immunology 117:301.
  3. Kasinrerk, W. et al. (1992) J. Immunol. 149:847.
  4. Miyauchi, T. et al. (1991) J. Biochem. 110:770.
  5. Hanna, S.M. et al. (2003) BMC Biochem. 4:17.
  6. Riethdorf, S. et al. (2006) Int. J. Cancer 119:1800.
  7. Braundmeier, A.G. et al. (2006) J. Clin. Endocrinol. Metab. 91:2358.
  8. Tang, Y. et al. (2006) Mol. Cancer Res. 4:371.
  9. Quemener, C. et al. (2007) Cancer Res. 67:9.
  10. Wilson, M.C. et al. (2005) J. Biol. Chem. 280:27213.
  11. Xu, D. and M.E. Hemler (2005) Mol. Cell. Proteomics 4:1061.
  12. Tang, W. et al. (2004) Mol. Biol. Cell 15:4043.
  13. Arora, K. et al. (2005) J. Immunol. 175:517.
  14. Pushkarsky, T. et al. (2005) J. Biol. Chem. 280:27866.
  15. Egawa, N. et al. (2006) J. Biol. Chem. 281:37576.
  16. Sidhu, S.S. et al. (2004) Oncogene 23:956.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EMMPRIN/CD147 Antibody (AF972)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA Capture, ELISA Development, IHC, IHC-P, Immunoprecipitation, Western Blot.


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ELISA Capture
(1)
ELISA Development
(1)
IHC
(1)
IHC-P
(3)
Immunoprecipitation
(2)
Western Blot
(1)
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Human
(8)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using AF972 Applications Species
N Sun, YT Lee, RY Zhang, R Kao, PC Teng, Y Yang, P Yang, JJ Wang, M Smalley, PJ Chen, M Kim, SJ Chou, L Bao, J Wang, X Zhang, D Qi, J Palomique, N Nissen, SB Han, S Sadeghi, RS Finn, S Saab, RW Busuttil, D Markovic, D Elashoff, HH Yu, H Li, AP Heaney, E Posadas, S You, JD Yang, R Pei, VG Agopian, HR Tseng, Y Zhu Purification of HCC-specific extracellular vesicles on nanosubstrates for early HCC detection by digital scoring Nat Commun, 2020-09-07;11(1):4489. 2020-09-07 [PMID: 32895384] (ELISA Capture, Human) ELISA Capture Human
M Aoki, K Koga, M Miyazaki, M Hamasaki, N Koshikawa, M Oyama, H Kozuka-Hat, M Seiki, BP Toole, K Nabeshima CD73 complexes with emmprin to regulate MMP-2 production from co-cultured sarcoma cells and fibroblasts BMC Cancer, 2019-09-12;19(1):912. 2019-09-12 [PMID: 31510956] (Immunoprecipitation, Human) Immunoprecipitation Human
A Sugyo, AB Tsuji, H Sudo, M Koizumi, Y Ukai, G Kurosawa, Y Kurosawa, T Saga, T Higashi Efficacy Evaluation of Combination Treatment Using Gemcitabine and Radioimmunotherapy with 90Y-Labeled Fully Human Anti-CD147 Monoclonal Antibody 059-053 in a BxPC-3 Xenograft Mouse Model of Refractory Pancreatic Cancer Int J Mol Sci, 2018-09-29;19(10):. 2018-09-29 [PMID: 30274301] (IHC, Human) IHC Human
D Gong, X Chi, K Ren, G Huang, G Zhou, N Yan, J Lei, Q Zhou Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin Nat Commun, 2018-09-06;9(1):3623. 2018-09-06 [PMID: 30190470] (Western Blot, Human) Western Blot Human
Cryopreserved Mesenchymal Stromal Cells Maintain Potency in a Retinal Ischemia/Reperfusion Injury Model: Toward an off-the-shelf Therapy Sci Rep, 2016-05-23;6(0):26463. 2016-05-23 [PMID: 27212469] (IHC-P, Mouse) IHC-P Mouse
Venkatesan B, Valente AJ, Reddy VS, Siwik DA, Chandrasekar B Resveratrol blocks interleukin-18-EMMPRIN cross-regulation and smooth muscle cell migration. Am. J. Physiol. Heart Circ. Physiol., 2009-06-26;297(2):H874-86. 2009-06-26 [PMID: 19561311] (ELISA Development, Human) ELISA Development Human
Koga K, Nabeshima K, Aoki M, Kawakami T, Hamasaki M, Toole BP, Nakayama J, Iwasaki H Emmprin in epithelioid sarcoma: expression in tumor cell membrane and stimulation of MMP-2 production in tumor-associated fibroblasts. Int. J. Cancer, 2007-02-15;120(4):761-8. 2007-02-15 [PMID: 17131322] (IHC-P, Human) IHC-P Human
Nabeshima K, Iwasaki H, Nishio J, Koga K, Shishime M, Kikuchi M Expression of emmprin and matrix metalloproteinases (MMPs) in peripheral nerve sheath tumors: emmprin and membrane-type (MT)1-MMP expressions are associated with malignant potential. Anticancer Res., 2006-03-01;26(2):1359-67. 2006-03-01 [PMID: 16619545] (IHC-P, Human) IHC-P Human
Pushkarsky T, Zybarth G, Dubrovsky L, Yurchenko V, Tang H, Guo H, Toole B, Sherry B, Bukrinsky M CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A. Proc. Natl. Acad. Sci. U.S.A., 2001-05-15;98(11):6360-5. 2001-05-15 [PMID: 11353871] (Immunoprecipitation, Human) Immunoprecipitation Human

Review for EMMPRIN/CD147 Antibody (AF972) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF972:
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(1)
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Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/12/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 24527394
SpeciesMouse

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Application: IHC-Fr
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Gene Symbol BSG
Uniprot