Synaptophysin Antibody (SYP/7976R) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human adrenal stained with Synaptophysin antibody (SYP/7976R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: IHC
• Clone: SYP/7976R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Synaptophysin Antibody (SYP/7976R) - Azide and BSA Free Summary

• Description: Positive Controls: HepG2, HeLa or Y79 cells. Human pancreas, cerebellum or pheochromocytoma.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment (around aa 274-313) of human Synaptophysin protein (exact sequence is proprietary)
• Localization: Cytoplasm.
• Specificity: This recombinant rabbit monoclonal antibody recognizes a protein of 38kDa that is identified as synaptophysin It is an N-glycosylated integral membrane protein found in neurons and endocrine cells Synaptophysin contains four transmembrane domains and may function as a gap junction-like channel This antibody identifies normal neuroendocrine cells and neuroendocrine neoplasms Diffuse, finely granular, cytoplasmic staining is observed, which probably correlates with the distribution of the antigen within neurosecretory vesicles Synaptophysin is an independent, broad-range marker of neural and neuroendocrine differentiation
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SYP
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Synaptophysin Antibody (SYP/7976R) - Azide and BSA Free

• Major synaptic vesicle protein p38
• MRX
• MRXSYP
• Synaptophysin
• SYP

Background

Synaptophysin (Syp I), also called the major synaptic vesicle protein p38, is an integral membrane glycoprotein found in the synaptic vesicles of brain, neuron and neuroendocrine cells. Synaptophysin is one of the most abundant membrane proteins, comprising approximately 7% of the total protein in small synaptic vesicles. In mature nerve terminals it forms a complex with the vesicular membrane protein synaptobrevin, which appears to modulate synaptobrevin's interaction with the plasma membrane-associated proteins syntaxin and SNAP25 to form the SNARE complex as a prerequisite for membrane fusion. Synaptophysin may also be used as a neuroendocrine tumor marker in both neuroscience and cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SYP