Western Blot: SREBP1 Antibody [NB100-2215] - Detection of SREBP1 using NB100-2215. Lane 1: human liver Lane 2: mouse liver
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-2215] - SREBP1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: SREBP1 Antibody [NB100-2215] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse liver with SREBP1 antibody at 1:200 dilution. The antibody generated an expected ...read more
Simple Western: SREBP1 Antibody [NB100-2215] - Simple Western lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Tris-Glycine and 0.15M NaCl
0.02% Sodium Azide
Immunogen affinity purified
Western Blot 1:500
Simple Western 1:25
Immunohistochemistry 1:200 - 1:500
Immunohistochemistry-Paraffin 1:200 - 1:500
In WB, this SREBP1 antibody detects the processed form of sterol regulatory element-binding protein 1 (aa 1-490) which runs at ~65kDa position. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
65 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Immunogen has 94% homology to goat, and sheep. Bovine reactivity reported in scientific literature (PMID: 25558823). Plant reactivity reported in scientific literature (PMID: 24649190)
Unprocessed SREBP1 is an ~122 kDa integral membrane protein that moves from ER to golgi for processing. The first cleavage is performed by S2P at residue 490, and the resulting active portion is then translocated out of the golgi. This is the ~65 kDa mature form that is detected by NB100-2215. The second cleavage is performed by S1P at residue 530, and the resulting C-term portion (from residues 530-1147) remains in the golgi where it is detected as a ~65 kDa protein by NB100-60545.
Alternate Names for SREBP1 Antibody
Class D basic helix-loop-helix protein 1
sterol regulatory element binding protein-1
sterol regulatory element binding transcription factor 1
SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for SREBP1 Antibody (NB100-2215). (Showing 1 - 2 of 2 FAQs).
I am looking for SREBF1 antibodies that can cross-react with zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebrafish or have high sequence identity in the immunogen region.
Unfortunately we do not have any antibodies that have been tested in zebrafish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebrafish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovators Reward Program. NB600-582 - 301-407 aa, NB100-2215 - 300-400 aa, NB100-60545 - 700-800 aa.
What dilution should I use for IHC-P?
The starting dilution for IHC-P testing with this antibody is 1:200 and the customer can play around the final working dilution depending upon the level of SREBP1 expression in his/her particular tissues. Moreover, SREBP1 is highly expressed in Liver and Adrenal glands, and a section of one of these tissues may be included as positive control in the assay.