SREBP1 Antibody

Images

 
Western Blot: SREBP1 Antibody [NB100-2215] - Detection of SREBP1 using NB100-2215. Lane 1: human liver Lane 2: mouse liver
Immunocytochemistry/ Immunofluorescence: SREBP1 Antibody [NB100-2215] - SREBP1 antibody was tested in HeLa cells with DyLight488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 ...read more
Immunohistochemistry-Paraffin: SREBP1 Antibody [NB100-2215] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse liver with SREBP1 antibody at 1:200 dilution. The antibody generated an expected ...read more
Simple Western: SREBP1 Antibody [NB100-2215] - Simple Western lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pl, Gt, ShSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SREBP1 Antibody Summary

Immunogen
A synthetic peptide made to a portion of the human SREBP1 protein sequence (between residues 300-400). [UniProt# P36956]
Localization
Membranes of ER, Golgi and Cytoplasmic Vesicles. Processed SREBP1 (1-490 a.a.) translocates to the nucleus.
Predicted Species
Goat (94%), Sheep (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SREBF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
Application Notes
In WB, this SREBP1 antibody detects the processed form of sterol regulatory element-binding protein 1 (aa 1-490) which runs at approx. 65kDa position.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
65 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Liver Lysate (NB820-59662)
Control Peptide
SREBP1 Peptide (NB100-2215PEP)
Reviewed Applications
Read 2 Reviews rated 4
using
NB100-2215 in the following applications:

Publications
Read Publications using
NB100-2215 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    6 publications

Reactivity Notes

Immunogen has 94% homology to goat, and sheep. Bovine reactivity reported in scientific literature (PMID: 25558823). Plant reactivity reported in scientific literature (PMID: 24649190)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

Unprocessed SREBP1 is an ~122 kDa integral membrane protein that moves from ER to golgi for processing. The first cleavage is performed by S2P at residue 490, and the resulting active portion is then translocated out of the golgi. This is the ~65 kDa mature form that is detected by NB100-2215. The second cleavage is performed by S1P at residue 530, and the resulting C-term portion (from residues 530-1147) remains in the golgi where it is detected as a ~65 kDa protein by NB100-60545.

Alternate Names for SREBP1 Antibody

  • BHLHD1
  • Class D basic helix-loop-helix protein 1
  • SREBP 1c
  • SREBP-1
  • SREBP1bHLHd1SREBP-1c
  • sterol regulatory element binding protein-1
  • sterol regulatory element binding transcription factor 1
  • sterol regulatory element-binding protein 1
  • Sterol regulatory element-binding transcription factor 1

Background

SREBP1 (sterol regulatory element binding protein 1, also called SREBF1 or BHLHD1) is a bHLH-LZ (basic helix-loop-helix-leucine zipper) transcription factor that acts as a key lipogenic transcription factor controlled by glucose/insulin and preferentially regulates lipogenesis via activating genes involved in fatty acid as well as triglyceride synthesis. It is a transcriptional activator required for lipid homeostasis and regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. SREBP1 has a dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (sterol regulatory element 1; 5'-ATCACCCCAC-3'). It exists as multi-pass membrane protein in the membranes of ER, Golgi apparatus and cytoplasmic vesicle /COPII-coated vesicles and moves from ER to Golgi in the absence of sterols. It forms a tight complex with SCAP in ER membranes and interacts with LMNA. AMPK phosphorylate SREBP1 leading to suppression of protein processing and nuclear translocation, and target gene expression repression. Moreover, SIK1 mediated phosphorylation at Ser-402 site represses its activity through inhibition of DNA-binding.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-2215
Species: Hu, Mu, Rt, Po, Bv, Pl, Gt, Sh
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for SREBP1 Antibody (NB100-2215)(10)

We have publications tested in 5 confirmed species: Mouse, Rat, Bovine, Hamster, Plant.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(6)
All Applications
Filter By Species
Mouse
(3)
Rat
(1)
Bovine
(1)
Hamster
(1)
Plant
(1)
All Species
Showing Publications 1 - 10 of 10.
Publications using NB100-2215 Applications Species
Kachaylo E, Tschuor C, Calo N et al. PTEN downregulation promotes b-oxidation to fuel hypertrophic liver growth after hepatectomy in mice. Hepatology Apr 24 2017 [PMID: 28437835] (Mouse) Mouse
Deng Q, Liu G, Liu L et al. BHBA influences bovine hepatic lipid metabolism via AMPK signaling pathway J. Cell. Biochem. 2015 Jan 05 [PMID: 25558823] (WB, ICC/IF, Bovine) WB, ICC/IF Bovine
Johnston-Cox Hillary, Eisenstein Anna S, Koupenova Milka et al. The macrophage A2B adenosine receptor regulates tissue insulin sensitivity. PLoS One. 2014 Jun 03 [PMID: 24892847] (WB) WB
Eser PO, Vanden Heuvel JP, Araujo J et al. Marine- and plant-derived omega-3 fatty acids differentially regulate prostate cancer cell proliferation. Mol Clin Oncol 2013 May [PMID: 24649190] (IHC, Plant) IHC Plant
Jelinek D, Castillo JJ, Richardson LM et al. The Niemann-Pick C1 Gene Is Downregulated in Livers of C57BL/6J Mice by Dietary Fatty Acids, but Not Dietary Cholesterol, through Feedback Inhibition of the SREBP Pathway J Nutr 2012 Sep 18 [PMID: 22990467] (WB, Mouse) WB Mouse
Yang A, Gyulay G, Mitchell M et al. Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice J Lipid Res 2012 Dec [PMID: 22984145]
Johnston-Cox H, Koupenova M, Yang D et al. The A2b adenosine receptor modulates glucose homeostasis and obesity. PLoS One. 2012 Jul 25 [PMID: 22848385] (WB, Mouse) WB Mouse
Johnson KJ, McDowell EN, Viereck MP, Xia JQ. Species-specific dibutyl phthalate fetal testis endocrine disruption correlates with inhibition of SREBP2-dependent gene expression pathways. Toxicol Sc 2011 Apr [PMID: 21266533] (WB, Rat)

Details:
Using the DyLight 550 conjugated version of NB100-2215, catalog number NB100-2215R.
WB Rat
Inoue SI, Yoshinari K, Sugawara M, Yamazoe Y. Activated Sterol Regulatory Element-Binding Protein-2 Suppresses Hepatocyte Nuclear Factor-4-Mediated Cyp3a11 Expression in Mouse Liver. Mol Pharmacol. 2010 Oct 12. [PMID: 20926756]
Yang TH, Yao HT, Chiang MT. Red algae (Gelidium amansii) hot-water extract ameliorates lipid metabolism in hamsters fed a high-fat diet. Journal of Food and Drug Analysis. Feb 14 2017 (WB, Hamster) WB Hamster

Reviews for SREBP1 Antibody (NB100-2215) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Mouse.
We have 2 reviews tested in 2 application: Simple Western, WB.

Reviews using NB100-2215:
Filter by Applications
Simple Western
(1)
WB
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western SREBP1 NB100-2215
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reviewed by:
Zachary Patinkin
Simple Western Human 06/14/2015
View

Summary

ApplicationSimple Western
Sample TestedHuman mesenchymal stem cell whole cell lysate
SpeciesHuman
LotF-1

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio1:25, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionProtein Simple Anti-Rabbit Secondary
Secondary Manufacturer Cat#042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Comments

CommentsAntibody has significant background, although can be easily adjusted using Compass software.
 
reviewed by:
Anonymous
WB Mouse 12/12/2014
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22990467
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22990467

Details

Detection NotesSee PMID 22990467

Comments

CommentsPublished in PMID: 22990467

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SREBP1 Antibody (NB100-2215). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for SREBF1 antibodies that can cross-react with zebrafish srebf1. I am wondering whether you have any SREBF1 antibodies that have been tested for zebrafish or have high sequence identity in the immunogen region.
    • Unfortunately we do not have any antibodies that have been tested in zebrafish. I have included the immunogen sequence for the antibodies it might be worth considering. I am not sure exactly what the zebrafish sequence is, I was unable to find it on uniprot.org, so I was unable to check the homology. The following antibodies all have available sample sizes. If you decide to try one you would qualify for our Innovators Reward Program. NB600-582 - 301-407 aa, NB100-2215 - 300-400 aa, NB100-60545 - 700-800 aa.
  2. What dilution should I use for IHC-P?
    • The starting dilution for IHC-P testing with this antibody is 1:200 and the customer can play around the final working dilution depending upon the level of SREBP1 expression in his/her particular tissues. Moreover, SREBP1 is highly expressed in Liver and Adrenal glands, and a section of one of these tissues may be included as positive control in the assay.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-2215AF405
Alexa Fluor 488 Labeled NB100-2215AF488
Alexa Fluor 647 Labeled NB100-2215AF647
Alexa Fluor 700 Labeled NB100-2215AF700
Biotin Labeled NB100-2215B
DyLight 405 Labeled NB100-2215V
DyLight 488 Labeled NB100-2215G
DyLight 550 Labeled NB100-2215R
DyLight 650 Labeled NB100-2215C
FITC Labeled NB100-2215F
HRP Labeled NB100-2215H

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Blogs on SREBP1.

SREBP2 - regulating cholesterol homeostasis and lipid metabolism
Sterol regulatory element-binding proteins (SREBP) are important transcription factors regulating the synthesis and uptake of lipids including cholesterol. This essential role in lipid metabolism makes investigations into the functions SREBPs im...  Read full blog post.

SREBP: Gatekeeper of Cholesterol Homeostasis
SREBP1 (sterol-regulatory-element-binding protein 2) is a basic-helix-loop-helix-leucine zipper (bHLH-ZIP) transcription factor. It regulates sterol and cholesterol homeostasis by controlling enzymes involved in cholesterol synthesis and uptake, e.g....  Read full blog post.

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Recent Reviews

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Zachary Patinkin
06/14/2015
Application: Simple Western
Species: Human

 
Anonymous
12/12/2014
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SREBF1