CHREBP Antibody


Western Blot: CHREBP Antibody [NB400-135] - Detection of ChREBP in liver nuclear extracts from well-fed rats. 7% SDS gel, 1:1,000 dilution of NB400-135. Photo courtesy of Dr. Uyeda, UT Southwestern University.
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-135] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were more
Immunohistochemistry: CHREBP Antibody [NB400-135] - Colon, Epithelium 40X.
Western Blot: CHREBP Antibody [NB400-135] - Detection of ChREBP in 20 ug of human hepatocyte lysate using NB400-135. 5-10 second film exposure.
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-135] - Immunofluorescent staining of a human hepatocyte using NB400-135. Overlay staining of ChREBP (rhodamine red) and DAPI nuclear staining (blue).
Immunohistochemistry: CHREBP Antibody [NB400-135] - Analysis of CHREBP in mouse liver using DAB with hematoxylin counterstain.

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, IB, ICC/IF, IHC, IHC-P, IP
1 mg/ml

CHREBP Antibody Summary

A C-terminal synthetic peptide made to the human CHREBP protein sequence (between residues 800-852). [UniProt# Q9NP71, Isoform 1/Alpha]
Immunogen affinity purified
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Packaging, Storage & Formulations

Store at 4C. Do not freeze.
0.02% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:1000
  • Chromatin Immunoprecipitation 1:10-1:500
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100-1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10-1:500
Application Notes
This CHREBP antibody is useful in ChIP (PMID: 21282101), Immunohistochemistry paraffin embedded sections, Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~95 kDa. Use in immunoblotting reported in scientific literature (PMID 26181104) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
95 kDa
Control Peptide
CHREBP Peptide (NB400-135PEP)
Reviewed Applications
Read 4 Reviews rated 4
NB400-135 in the following applications:

Read Publications using
NB400-135 in the following applications:

  • 17 publications
  • 1 publication
  • 3 publications
  • IP
    4 publications
  • WB
    34 publications

Reactivity Notes

Human, rat and mouse (PMID 21835137).

Alternate Names for CHREBP Antibody

  • BHLHD14
  • CHREBPcarbohydrate-responsive element-binding protein
  • Class D basic helix-loop-helix protein 14
  • MIOWS basic-helix-loop-helix leucine zipper protein
  • MLX interacting protein-like
  • MLX interactor
  • MLX-interacting protein-like
  • MONDOBWilliams-Beuren syndrome chromosomal region 14 protein
  • WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1
  • Williams Beuren syndrome chromosome region 14
  • Williams-Beuren syndrome chromosome region 14 protein 2
  • WS-bHLHbHLHd14carbohydrate response element binding protein


Carbohydrate response element-binding protein (ChREBP) is a glucose sensitive basic helix-loop-helix/leucine zipper transcription factor which heterodimerise with MLX and binds to carbohydrate response element (ChoRE) on DNA for the induction genes involved in glycolysis, de novo lipogenesis (DNL), and fatty acid desaturation. Some of the target genes of ChREBP are glucokinase (GCK), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), pyruvate kinase/liver type pyruvate kinase (PK1/ PKLR), delta-9-desaturase (SCD/SCD1) etc. ChREBP is expressed as alpha and beta alternatively spliced isoforms, and among these two isoforms, the alpha-isoform is activated in a glucose-dependent manner. ChREBP is expressed in the cytoplasm and nuclei of a number of mammalian tissues, with high levels found in liver, skeletal muscle, white/brown adipose, heart, kidney, cerebellum and intestine. Glucose stimulates ChREBP transcriptional expression and activates the transactivity of ChREBP protein. It is activated independent of insulin and recent findings have suggested that together with its binding partner Mlx, ChREBP implicates in metabolic processes, insulin signaling related pathways, and also tumorigenesis/cancers. Also, increased hepatic ChREBP expression has been suggested to result in development of non-alcoholic fatty liver disease (NAFLD) and obesity through the conversion of carbohydrates into triglycerides (de novo lipogenesis).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
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Applications: WB, IHC, IP, ChIP, DirELISA, GS
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Applications: WB, IHC
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Applications: WB, Simple Western, IHC, ICC

Publications for CHREBP Antibody (NB400-135)(73)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: ChIP, EMSA, ICC/IF, IP, WB.

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Showing Publications 1 - 10 of 73. Show All 73 Publications.
Publications using NB400-135 Applications Species
Marmugi A, Lukowicz C, Lasserre F et al. Activation of the Constitutive Androstane Receptor induces hepatic lipogenesis and regulates Pnpla3 gene expression in a LXR-independent way. Toxicol. Appl. Pharmacol. May 11 2016 12:00AM [PMID:27180240] (WB, Mouse) WB Mouse
Tang Y, Wallace M, Sanchez-Gurmaches J et al. Adipose tissue mTORC2 regulates ChREBP-driven de novo lipogenesis and hepatic glucose metabolism. Nat Commun. 2016 Apr 21 [PMID:27098609] (WB, Mouse) WB Mouse
Kitsunai H, Makino Y, Sakagami H et al. High glucose induces platelet-derived growth factor-C via carbohydrate response element-binding protein in glomerular mesangial cells. Physiol Rep. 2016 Mar 01 [PMID:27033449] (ChIP, Human) ChIP Human
Zhang Z, Li B, Meng X et al. Berberine prevents progression from hepatic steatosis to steatohepatitis and fibrosis by reducing endoplasmic reticulum stress. Sci Rep. 2016 Feb 09 [PMID:26857750] (WB, Mouse) WB Mouse
Nuotio-Antar AM, Poungvarin N, Li M et al. FABP4-Cre Mediated Expression of Constitutively Active ChREBP Protects Against Obesity, Fatty Liver, and Insulin Resistance. Endocrinology 2015 Aug 06 [PMID:26248218] (WB, Mouse) WB Mouse
Witte N, Muenzner M, Rietscher J et al. The glucose sensor ChREBP links de-novo lipogenesis to PPARgamma activity and adipocyte differentiation. Endocrinology 2015 Jul 16 [PMID:26181104] (WB) WB
Li X, Kover KL, Heruth DP et al. New Insight Into Metformin Action: Regulation of ChREBP and FOXO1 Activities in Endothelial Cells Mol. Endocrinol. 2015 Aug 01 [PMID:26147751] (ICC/IF, Human, Mouse) ICC/IF Human, Mouse
Trabelsi MS, Daoudi M, Prawitt J et al. Farnesoid X receptor inhibits glucagon-like peptide-1 production by enteroendocrine L cells Nat Commun. 2015 Jul 03 [PMID:26134028] (WB, Mouse) WB Mouse
Marmier S, Dentin R, Daujat-Chavanieu M et al. Novel role for ChREBP in the control of ethanol metabolism and susceptibility to binge drinking. Hepatology. 2015 Mar 11 [PMID:25761756] (WB, IP, Human) WB, IP Human
Poungvarin N, Chang B, Imamura M et al. Genome-Wide Analysis of ChREBP Binding Sites on Male Mouse Liver and White Adipose Chromatin. Endocrinology. 2015 Mar 09 [PMID:25751637] (ChIP, Mouse) ChIP Mouse
Show All 73 Publications.

Reviews for CHREBP Antibody (NB400-135) (4) 44

Average Rating: 4
(Based on 4 reviews)
We have 4 reviews tested in 3 species: Human, Mouse, Rat.
We have 4 reviews tested in 2 application: WB, ChIP.

Reviews using NB400-135:
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Images Ratings Applications Species Date Details
reviewed by:
WB Human 01/15/2013


ApplicationWestern Blot
Sample Testedhuman fibroblast whole cell extract


Blocking Details5% nonfat milk in TBST, 30 min at RT

Primary Anitbody

Dilution Ratio1:200 in 2% BSA/TBST, overnight at 4C

Secondary Antibody

Secondary Descriptiongoat anti-rabbit IgG, HRP
Secondary Manufacturer Cat#Thermo Scientific, #31462
Secondary Concentration1:10,000


Detection NotesLumi-light
reviewed by:
WB 12/13/2012


ApplicationWestern Blot
Special ApplicationsUsed on mouse liver lysates. Detection in cytosol and nuclear fraction by Western blot.
Pub Med ID22927645
reviewed by:
ChIP Rat 11/28/2012


ApplicationChromatin Immunoprecipitation
Sample TestedRat hepatocytes
CommentsWe found that this antibody worked very well for ChIP, however in our cells (primary rat hepatocytes) we could not detect ChREBP protein by Western blotting using this antibody. We are open to any suggestions that could help us detect ChREBP protein by Western blotting?

Primary Anitbody

Dilution RatioChREBP (6ug)


Detection NotesRT-PCR
Fixation/Cross-Linking5M NaCl, 4hr incubation at 65 degrees Celsius
Immunoprecipitation DetailsProtein A agarose beads, 4hr incubation at 4 degrees Celcius
Crosslinking/Reversal Details1% formaldehyde
DNA Prurification DetailsPhenol chloroform


CommentsWe found that this antibody worked very well for ChIP, however in our cells (primary rat hepatocytes) we could not detect ChREBP protein by Western blotting using this antibody. We are open to any suggestions that could help us detect ChREBP protein by Western blotting?
Western Blot CHREBP NB400-135
reviewed by:
WB Mouse 02/02/2011


ApplicationWestern Blot
Sample TestedAorta whole tissue, Sample Amount: 20ug
CommentsLoaded 20ug total protein, nitrocellulose membrane, blocked with milk, primary antibody dilution of 1:1000 and secondary dilution of 1;10000. Some non-specifics band at 75kDa also visible


Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 2 hours, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000 in milk, Primary Ab Incubation Time: overnight, Primary Ab Incubation Temp: 4 degrees Celsius

Secondary Antibody

Secondary DescriptionSecondary Ab: Goat Anti-Mouse IgG HRP, Secondary Ab Dilution Ratio: 1:10000 in milk
Secondary Manufacturer Cat#sc-2005


Detection NotesDetection Method: Supersignal Pierce, Exposure Time: 20 min, Specific Bands: 95kDa, Non-Specific Bands: 75kDa


CommentsLoaded 20ug total protein, nitrocellulose membrane, blocked with milk, primary antibody dilution of 1:1000 and secondary dilution of 1;10000. Some non-specifics band at 75kDa also visible

Product General Protocols

View specific protocols for CHREBP Antibody (NB400-135): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for CHREBP Antibody (NB400-135). (Showing 1 - 1 of 1 FAQs).

  1. Pplease I want to block ChREBP Ab (NB400-135) using ChREBP peptide I need help to no the procedure and how much I need to add from the peptide to the ChREBP Ab
    • Here is a protocol for a blocking peptide control for a western blot: Materials and Reagents Blocking buffer (usually TBST plus either 5% non-fat dry milk or 3% BSA for Western blot, or PBS plus 1% BSA for IHC) - Antibody - Blocking (immunizing) peptide - Two tubes - Two identical samples (e.g. a Western blot with two identical lanes, cut in half; two slides containing the cells of interest; etc) Method 1. Determine the optimal concentration of antibody that consistently gives a positive result in your particular protocol. Using that concentration, determine how much antibody you will need for two experiments. a. For example, an antibody is being used successfully in Western blot at 0.5 ug/ml. You will need 2 ml of antibody solution to stain one strip of a Western blot. Thus, you would use 1 ug of antibody in 2 ml buffer for each strip. 2. Dilute the necessary amount of antibody in blocking buffer to the final volume needed for the two experiments. Divide this equally into two tubes. 3. In the first tube, labeled locked, add the blocking peptide to a final concentration of 1 ug/ml (2 ug total peptide in this example). In the second tube, labeled Control, add an equivalent amount of buffer. 4. Incubate both tubes, with agitation, at room temperature for 30 minutes, or overnight at 4 degrees C. 5. Perform the staining protocol on the two identical samples, using the blocked antibody for one and the control for the other. Be careful not to mix up the strips using the blocked and control antibodies! 6. Observe the staining. The staining that disappears when using the blocked antibody is specific to the antibody. (See note i) Notes i. If more than one band disappears in Western blot by peptide/antigen competition, those bands contain the antigenic determinants and could be fragments of the full antigen or a complex containing the antigen.

Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NB400-135AF405
Alexa Fluor (R) 488 Labeled NB400-135AF488
Alexa Fluor (R) 647 Labeled NB400-135AF647
Alexa Fluor (R) 700 Labeled NB400-135AF700
Biotin Labeled NB400-135B
DyLight 405 Labeled NB400-135V
DyLight 488 Labeled NB400-135G
DyLight 550 Labeled NB400-135R
DyLight 650 Labeled NB400-135C
FITC Labeled NB400-135F
HRP Labeled NB400-135H

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Blogs on CHREBP.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in...  Read full blog post.

ChREBP and Fatty Acid Biosynthesis Regulation
Carbohydrate response element binding protein (ChREBP) is a transcription factor involved in activating genes that encode enzymes of fatty acid biosynthesis in liver and adipose. It is believed to be a key controller of hepatic lipogenesis and has a p...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol MLXIPL

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