CHREBP Antibody - BSA Free

Images

 
Western Blot: CHREBP Antibody [NB400-135] - Detection of ChREBP in liver nuclear extracts from well-fed rats. 7% SDS-PAGE gel, 1:1000 dilution of NB400-135. Photo courtesy of Dr. Uyeda, UT Southwestern University.
Immunohistochemistry: CHREBP Antibody [NB400-135] - Analysis of CHREBP in mouse liver using DAB with hematoxylin counterstain.
Western Blot: CHREBP Antibody [NB400-135] - Mouse liver tissue lysate, 10 ug total protein per lane, probed with CHREBP antibody. Specific bands at ~150 kDa (some wells as doublets). Weaker bands at ~45 kDa, very faint ...read more
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-135] - RetSat depletion prevents the glucose-induced nuclear accumulation of ChREBP independent of 13,14-dihydroretinol generation. Hepatocytes were ...read more
Immunohistochemistry-Paraffin: CHREBP Antibody [NB400-135] - Staining of human colon, epithelium at a 40X magnification.
Chromatin Immunoprecipitation: CHREBP Antibody [NB400-135] - Effects of glucose on ChREBP binding.HepG2 cells were treated with low (2.7 mM) and high (25 mM) glucose for 8 h. Chromatin was isolated and fragmented, and ...read more
Western Blot: CHREBP Antibody [NB400-135] - Detection of ChREBP in 20 ug of human hepatocyte lysate using NB400-135. 5-10 second film exposure.
Western Blot: CHREBP Antibody [NB400-135] - RetSat depletion in mouse liver reduces protein levels and target gene expression of ChREBP. RetSat and ChREBP protein expression by immunoblotting. Image collected and ...read more
Western Blot: CHREBP Antibody [NB400-135] - Protein expression levels of carbohydrate-responsive element-binding protein (ChREBP), in livers of rats receiving normal water (CON), a 13% (w/v) glucose solution (GLU), a ...read more
Immunocytochemistry/ Immunofluorescence: CHREBP Antibody [NB400-135] - HeLa cells were fixed for 10 minutes using 10% formalin and permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP, PAGE, KO
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

CHREBP Antibody - BSA Free Summary

Immunogen
A C-terminal synthetic peptide made to the human CHREBP protein sequence (between residues 800-852). [UniProt# Q9NP71, Isoform 1/Alpha]
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MLXIPL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation 1:10 - 1:500
  • Gel Super Shift Assays
  • Immunocytochemistry/Immunofluorescence 1:100 - 1:500
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Immunoprecipitation 1:10 - 1:500
  • Knockout Validated
  • SDS-Page
  • Western Blot 1:1000
Application Notes
Use in SDS-PAGE reported in scientific literature (PMID:35041621) Use in ChIP reported in scientific literature (PMID: 21282101). Use in Gel super shift assays reported in scientific literature (PMID: 20025850). Use in immunoblotting reported in scientific literature (PMID: 26181104). Knockout validation reported in scientific literature (PMID: 31668386).
Theoretical MW
95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
CHREBP Peptide (NB400-135PEP)
Reviewed Applications
Read 7 Reviews rated 4
using
NB400-135 in the following applications:

Publications
Read Publications using
NB400-135 in the following applications:

Reactivity Notes

Use in Mouse reported in scientific literature (PMID: 33812059).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for CHREBP Antibody - BSA Free

  • BHLHD14
  • CHREBPcarbohydrate-responsive element-binding protein
  • Class D basic helix-loop-helix protein 14
  • MIOWS basic-helix-loop-helix leucine zipper protein
  • MLX interacting protein-like
  • MLX interactor
  • MLX-interacting protein-like
  • MONDOBWilliams-Beuren syndrome chromosomal region 14 protein
  • WBSCR14Williams-Beuren syndrome chromosome region 14 protein 1
  • Williams Beuren syndrome chromosome region 14
  • Williams-Beuren syndrome chromosome region 14 protein 2
  • WS-bHLHbHLHd14carbohydrate response element binding protein

Background

Carbohydrate response element-binding protein (ChREBP) is a glucose sensitive basic helix-loop-helix/leucine zipper transcription factor which heterodimerise with MLX and binds to carbohydrate response element (ChoRE) on DNA for the induction genes involved in glycolysis, de novo lipogenesis (DNL), and fatty acid desaturation. Some of the target genes of ChREBP are glucokinase (GCK), fatty acid synthase (FAS), acetyl-CoA carboxylase (ACC), pyruvate kinase/liver type pyruvate kinase (PK1/ PKLR), delta-9-desaturase (SCD/SCD1) etc. ChREBP is expressed as alpha and beta alternatively spliced isoforms, and among these two isoforms, the alpha-isoform is activated in a glucose-dependent manner. ChREBP is expressed in the cytoplasm and nuclei of a number of mammalian tissues, with high levels found in liver, skeletal muscle, white/brown adipose, heart, kidney, cerebellum and intestine. Glucose stimulates ChREBP transcriptional expression and activates the transactivity of ChREBP protein. It is activated independent of insulin and recent findings have suggested that together with its binding partner Mlx, ChREBP implicates in metabolic processes, insulin signaling related pathways, and also tumorigenesis/cancers. Also, increased hepatic ChREBP expression has been suggested to result in development of non-alcoholic fatty liver disease (NAFLD) and obesity through the conversion of carbohydrates into triglycerides (de novo lipogenesis).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB400-135
Species: Hu, Mu, Rt
Applications: WB, ChIP, GS, ICC/IF, IHC, IHC-P, IP, PAGE, KO

Publications for CHREBP Antibody (NB400-135)(126)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: ChIP, EMSA, ICC/IF, IHC-P, IP, KO, SDS-PAGE, WB.


Filter By Application
ChIP
(27)
EMSA
(1)
ICC/IF
(3)
IHC-P
(3)
IP
(6)
KO
(1)
SDS-PAGE
(1)
WB
(66)
All Applications
Filter By Species
Human
(19)
Mouse
(58)
Rat
(20)
All Species
Showing Publications 1 - 10 of 126. Show All 126 Publications.
Publications using NB400-135 Applications Species
Shibuya K, Ebihara K, Ebihara C et al. AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4 The Journal of biological chemistry Apr 14 2022 [PMID: 35430252] (WB, Mouse) WB Mouse
Balamurugan K, Medishetti R, Kotha J et al. PHLPP1 promotes neutral lipid accumulation through AMPK/ChREBP-dependent lipid uptake and fatty acid synthesis pathways iScience Feb 1 2022 [PMID: 35141506] (ChIP, Mouse) ChIP Mouse
Gu W, Wang X, Zhao H et al. Resveratrol ameliorates diabetic kidney injury by reducing lipotoxicity and modulates expression of components of the junctional adhesion molecule-like/sirtuin 1 lipid metabolism pathway European journal of pharmacology Jan 25 2022 [PMID: 35090936] (IHC-P, WB, Human, Mouse) IHC-P, WB Human, Mouse
Recazens E, Tavernier G, Dufau J et al. ChREBP beta is dispensable for the control of glucose homeostasis and energy balance JCI insight Jan 18 2022 [PMID: 35041621] (SDS-PAGE, Mouse) SDS-PAGE Mouse
Mendoza A, Tang C, Choi J Et al. Thyroid hormone signaling promotes hepatic lipogenesis through the transcription factor ChREBP Science signaling Nov 16 2021 [PMID: 34784250] (IP) IP
Yenilmez B, Wetoska N, Kelly M Et al. An RNAi therapeutic targeting hepatic DGAT2 in a genetically obese mouse model of nonalcoholic steatohepatitis Molecular therapy : the journal of the American Society of Gene Therapy Nov 11 2021 [PMID: 34774753] (WB) WB
Carroll PA, Freie BW, Cheng PF Et al. The glucose-sensing transcription factor MLX balances metabolism and stress to suppress apoptosis and maintain spermatogenesis PLoS biology Oct 1 2021 [PMID: 34669700] (WB, Mouse) WB Mouse
Chen N, Song S, Yang Z et al. ChREBP deficiency alleviates apoptosis by inhibiting TXNIP/oxidative stress in diabetic nephropathy Journal of Diabetes and its Complications Sep 1 2021 [PMID: 34600826]
Liu Y, Zienkiewicz J, Boyd KL et al. Hyperlipidemic hypersensitivity to lethal microbial inflammation and its reversal by selective targeting of nuclear transport shuttles Scientific reports Jun 7 2021 [PMID: 34099795] (WB) WB
Mori H, Dugan CE, Nishii A et al. The molecular and metabolic program by which white adipocytes adapt to cool physiologic temperatures PLoS biology May 1 2021 [PMID: 33979328] (WB, Mouse) WB Mouse
Show All 126 Publications.

Reviews for CHREBP Antibody (NB400-135) (7) 47

Average Rating: 4
(Based on 7 reviews)
We have 7 reviews tested in 3 species: Human, Mouse, Rat.

Reviews using NB400-135:
Filter by Applications
Western Blot
(5)
Immunohistochemistry-Frozen
(1)
Chromatin Immunoprecipitation
(1)
All Applications
Filter by Species
Human
(1)
Mouse
(3)
Rat
(2)
All Species
Images Ratings Applications Species Date Details
Western Blot CHREBP NB400-135
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4
reviewed by:
Erin Stephenson
Western Blot Mouse 07/01/2021
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Summary

ApplicationWestern Blot
Sample TestedC57BL/6J Liver,Liver,mouse liver lysate,Mouse Liver Lysates,Mouse Liver Tissue,Mouse Liver
SpeciesMouse
LotS-5

Comments

CommentsSDS-PAGE was performed using a Biorad mini-PROTEAN TGX 4-20% precast gel and separated proteins were transferred to a nitrocellulose membrane. Precision Plus Duel Standard ladder was added to either end and the middle of the gel. For samples, 10ug protein was loaded per well. After transfer the membrane was cut down the middle and each half was blocked for 1h at room temperature in TBST containing 3% BSA. The left half of the membrane was incubated at 4C overnight in NB400-135 (1:1000 prepared in TBST w/ 2.5% BSA), whereas the right half of the membrane was incubated at 4C overnight in both NB400-135 and blocking peptide (NB400-135PEP, 1ug/mL, prepared in TBST w/ 2.5% BSA). Membranes were washed in TBST, incubated in Alexa Fluor 488 anti-rabbit secondary antibody for 1h at room temperature (1:15,000 prepared in TBST w/ 3% BSA), washed again, and imaged using a BioRad ChemiDoc MP Imager.
Western Blot CHREBP NB400-135
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3
reviewed by:
Verified Customer
Western Blot Rat 06/16/2020
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Summary

ApplicationWestern Blot
Sample TestedRat Liver Nuclear Protein Extract
SpeciesRat
LotQ-3

Comments

Comments1:2000, overnight
Secondary antibody: 1:20000
Immunohistochemistry-Frozen CHREBP NB400-135
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5
reviewed by:
Ming Hua
Immunohistochemistry-Frozen Mouse 09/01/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Testedaortic tissues
SpeciesMouse
LotH1

Comments

CommentsThe frozen sections were permeabilized with 0.1% TritonX-100 and block with 10% goat serum. Sections were incubated with FITC-conjugated secondary antibodies.
  4
reviewed by:
Verified Customer
Western Blot Human 01/15/2013
View

Summary

ApplicationWestern Blot
Sample Testedhuman fibroblast whole cell extract
SpeciesHuman
LotH-2
  4
reviewed by:
Verified Customer
Western Blot 12/13/2012
View

Summary

ApplicationWestern Blot
LotC2
  4
reviewed by:
John Petrie
Chromatin Immunoprecipitation Rat 11/28/2012
View

Summary

ApplicationChromatin Immunoprecipitation
Sample TestedRat hepatocytes
SpeciesRat
Western Blot CHREBP NB400-135
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4
reviewed by:
Verified Customer
Western Blot Mouse 02/02/2011
View

Summary

ApplicationWestern Blot
Sample TestedAorta whole tissue, Sample Amount: 20ug
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for CHREBP Antibody (NB400-135). (Showing 1 - 1 of 1 FAQs).

  1. Pplease I want to block ChREBP Ab (NB400-135) using ChREBP peptide I need help to no the procedure and how much I need to add from the peptide to the ChREBP Ab
    • Here is a protocol for a blocking peptide control for a western blot: Materials and Reagents Blocking buffer (usually TBST plus either 5% non-fat dry milk or 3% BSA for Western blot, or PBS plus 1% BSA for IHC) - Antibody - Blocking (immunizing) peptide - Two tubes - Two identical samples (e.g. a Western blot with two identical lanes, cut in half; two slides containing the cells of interest; etc) Method 1. Determine the optimal concentration of antibody that consistently gives a positive result in your particular protocol. Using that concentration, determine how much antibody you will need for two experiments. a. For example, an antibody is being used successfully in Western blot at 0.5 ug/ml. You will need 2 ml of antibody solution to stain one strip of a Western blot. Thus, you would use 1 ug of antibody in 2 ml buffer for each strip. 2. Dilute the necessary amount of antibody in blocking buffer to the final volume needed for the two experiments. Divide this equally into two tubes. 3. In the first tube, labeled locked, add the blocking peptide to a final concentration of 1 ug/ml (2 ug total peptide in this example). In the second tube, labeled Control, add an equivalent amount of buffer. 4. Incubate both tubes, with agitation, at room temperature for 30 minutes, or overnight at 4 degrees C. 5. Perform the staining protocol on the two identical samples, using the blocked antibody for one and the control for the other. Be careful not to mix up the strips using the blocked and control antibodies! 6. Observe the staining. The staining that disappears when using the blocked antibody is specific to the antibody. (See note i) Notes i. If more than one band disappears in Western blot by peptide/antigen competition, those bands contain the antigenic determinants and could be fragments of the full antigen or a complex containing the antigen.

Secondary Antibodies

 

Isotype Controls

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Blogs on CHREBP.

Deficiency of GluT1 leads to neurological problems while excess is involved in cancers
By Jamshed Arslan, Pharm. D., PhD. What Are GluTs?Mammalian cell metabolism is incomplete without glucose   . Glucose is a monosaccharide that is transported to the cells through facilitative diffusion, a ...  Read full blog post.

Insulin signaling in adipocytes: Carbohydrate-signaling transcription factor ChREBP is the link between lipolytic enzyme Hormone-Sensitive Lipase and lipogenic enzyme ELOVL6
By Jamshed Arslan, Pharm. D., PhD. Insulin resistance in adipocytes is a major feature of metabolic syndrome   . Disrupted adipose tissue metabolism can lead to accumulation of lipid intermediates in insul...  Read full blog post.

ChREBP, a glucose sensitive transcription factor with role in glucose-lipids homeostasis and cancer
ChREBP (carbohydrate response element-binding protein) is a glucose responsive basic helix-loop-helix/leucine zipper (bHLH/LZ) transcription factor that binds MLX and then carbohydrate response element /ChoRE for the induction of genes involved in ...  Read full blog post.

ChREBP and Fatty Acid Biosynthesis Regulation
Carbohydrate response element binding protein (ChREBP) is a transcription factor involved in activating genes that encode enzymes of fatty acid biosynthesis in liver and adipose. It is believed to be a key controller of hepatic lipogenesis and has a p...  Read full blog post.

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Recent Reviews

4
5
1
4
5
3
1
2
0
1
0

Erin Stephenson
07/01/2021
Application: Western Blot
Species: Mouse

Verified Customer
06/16/2020
Application: Western Blot
Species: Rat

Ming Hua
09/01/2017
Application: Immunohistochemistry-Frozen
Species: Mouse

Bioinformatics

Gene Symbol MLXIPL