PPAR alpha/NR1C1 Antibody

Images

 
Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Affinity Purified Detection a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 ug of lysate was loaded per lane for ...read more
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody [NB600-636] - Rabbit Anti-PPAR alpha (N-terminal specific) antibody using (A) Mouse NIH/3T3 or (B) Human HEK293 cells fixed with MeOH. (C) Secondary ...read more
Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) ...read more
Western Blot: PPAR alpha/NR1C1 Antibody [NB600-636] - Lane 1: NIH/3T3. Load: 10 ug per lane. Primary antibody: PPAR Alpha (N-terminal specific) antibody at 1:1,000 for overnight at 4C. Secondary antibody: Peroxidase ...read more
Immunocytochemistry/ Immunofluorescence: PPAR alpha/NR1C1 Antibody [NB600-636] - Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 ...read more
Immunohistochemistry-Paraffin: PPAR alpha/NR1C1 Antibody [NB600-636] - Mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen ...read more
ELISA: PPAR alpha/NR1C1 Antibody [NB600-636] - ELISA results of purified Rabbit anti-PPAR Alpha (N-terminal specific) Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ChIP, ELISA, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PPAR alpha/NR1C1 Antibody Summary

Immunogen
PPAR alpha/NR1C1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha/NR1C1. (Uniprot: P23204)
Localization
Nuclear
Specificity
No reactivity is expected against other subtypes of PPAR.
Predicted Species
Human (92%), Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPARA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • Chromatin Immunoprecipitation 1:10-1:500
  • ELISA 1:75000-1:125000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1-5ug/ml
  • Immunohistochemistry 1:100-1:300
  • Immunohistochemistry-Paraffin 1:200
Application Notes
Expect a single band approximately 52 kDa in size corresponding to PPAR alpha/NR1C1 by western blot in the appropriate tissue or cell lysate. A 1:200 dilution is suggested for Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Use in chromatin immunoprecipitation reported in scientific literature (PMID 26586378).
Theoretical MW
52.35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NB600-636 in the following applications:

Reactivity Notes

A BLAST analysis was used to suggest reactivity with this protein from bovine, dog, golden hamster and boar sources based on 100% homology for the immunogen sequence. Cross reactivity with PPAR alpha/NR1C1 protein from chimpanzee and rhesus monkey may also occur as this sequence shows 88% homology (16/18 identities) with the protein from these sources. Cross reactivity with PPAR alpha/NR1C1 homologues from other sources has not been determined
Human reactivity reported in scientific literature (PMID: 31095524).

Packaging, Storage & Formulations

Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Notes

This antibody is directed against mouse PPAR alpha/NR1C1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for PPAR alpha/NR1C1 Antibody

  • alpha
  • hPPAR
  • NR1C1
  • NR1C1MGC2237
  • Nuclear receptor subfamily 1 group C member 1
  • peroxisome proliferator-activated receptor alpha
  • PPAR alpha
  • PPARA
  • PPARalpha
  • PPAR-alpha
  • PPARMGC2452

Background

Since their discovery in the early 1990's, the peroxisome proliferator activated receptors (PPARs) have attracted significant attention. This is primarily because PPARs serve as receptors for two very important classes of drugs: the hypolipidemic fibrates and the insulin sensitizing hiazolidinediones. Peroxisome proliferators are non-genotoxic carcinogens that are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family termed PPARs. Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Upon binding fatty acids or hypolipidemic drugs, PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate the expression of target genes. There are 3 known subtypes of PPARs: PPAR-alpha, PPAR-delta and PPARgamma. Mostly target genes are involved in the catabolism of fatty acids. Conversely, PPAR-gamma is activated by peroxisome proliferators such as prostaglandins, leukotrienes and anti-diabetic thiazolidinediones and affects the expression of genes involved in the storage of the fatty acids. PPAR-gamma may also be involved in adipocyte differentiation. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 through interaction with specific response elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PPAR alpha/NR1C1 Antibody (NB600-636)(17)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 3 applications: ChIP, ICC/IF, WB.


Filter By Application
ChIP
(3)
ICC/IF
(1)
WB
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(7)
Rat
(1)
Canine
(2)
All Species
Showing Publications 1 - 10 of 17. Show All 17 Publications.
Publications using NB600-636 Applications Species
Galiazzo G, Tagliavia C, Giancola F et al. Localisation of cannabinoid and cannabinoid-related receptors in the horse ileum Journal of Equine Veterinary Science Jun 1 2021
Kain J, Wei X, Reddy Na Et Al. Pioneer factor Foxa2 enables ligand-dependent activation of type II nuclear receptors FXR and LXR alpha Molecular metabolism Jul 8 2021 [PMID: 34246806] (ChIP) ChIP
Baskaran P, Nazminia K, Frantz J et al. Mice lacking endogenous TRPV1 express reduced levels of thermogenic proteins and are susceptible to diet-induced obesity and metabolic dysfunction FEBS letters May 11 2021 [PMID: 33977527]
Chiocchetti R, Rinnovati R, Tagliavia C et al. Localisation of cannabinoid and cannabinoid-related receptors in the equine dorsal root ganglia Equine Vet. J. Jun 11 2020 [PMID: 32524649]
Moore MC, Smith MS, Swift LL et al. Bromocriptine Mesylate Improves Glucose Tolerance and Disposal in a High-Fat-Fed Canine Model Am. J. Physiol. Endocrinol. Metab. May 27 2020 [PMID: 32459527] (Canine) Canine
Stanzani A, Galiazzo G, Giancola F et al. Localization of cannabinoid and cannabinoid related receptors in the cat gastrointestinal tract Histochem. Cell Biol. Feb 24 2020 [PMID: 32095931]
Chiocchetti R, Galiazzo G, Tagliavia C et al. Corrigendum: Cellular Distribution of Canonical and Putative Cannabinoid Receptors in Canine Cervical Dorsal Root Ganglia Front. Vet. Sci. 2019 Oct 31 [PMID: 31608295] (ICC/IF, Canine) ICC/IF Canine
Huang C, Zhang X, Jiang L et al. CBX5/G9a/H3K9me-mediated gene repression is essential to fibroblast activation during lung fibrosis JCI Insight May 16 2019 [PMID: 31095524] (WB, Mouse, Human) WB Mouse, Human
Baskaran P, Covington K, Bennis J et al. Binding Efficacy and Thermogenic Efficiency of Pungent and Nonpungent Analogs of Capsaicin. Molecules Dec 4 2018 [PMID: 30518154] (WB, Mouse) WB Mouse
Krishnan V, Baskaran P, Thyagarajan B. Troglitazone activates TRPV1 and causes deacetylation of PPARg in 3T3-L1 cells. Biochim Biophys Acta Mol Basis Dis. Nov 26 2018 [PMID: 30496795] (WB, Mouse) WB Mouse
Show All 17 Publications.

Reviews for PPAR alpha/NR1C1 Antibody (NB600-636) (0)

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar
ICC/IF Video Protocol

FAQs for PPAR alpha/NR1C1 Antibody (NB600-636). (Showing 1 - 1 of 1 FAQs).

  1. Please differentiate to me between PPAR and PGC clearly. I am confused with the difference between these two
    • Thank you very much for contacting Novus Biologicals technical support team and sharing your query on the differences between PGC-1 alpha and PPAR. These are two different proteins encoded by their respective genes and serves different functions. PGC-1 alpha (PGC1A or PPAR gamma coactivator 1-alpha) is a transcriptional co-activator for steroid receptors and nuclear receptors, and it regulates diverse aspects of cellular physiology. It up-regulates the transcriptional activity of PPAR-gamma /thyroid hormone receptor on the uncoupling protein promoter; regulates the key mitochondrial genes involved in adaptive thermogenesis; implicates in the metabolic reprogramming in response to nutrients availability through the coordination of the expression of a wide array of genes involved in the regulation of glucose and fatty acid metabolism. Among our PGC-1 alpha antibodies, NBP1-04676 is our best selling product with nice customer feedback and citations in at least 13 research publications. PPAR (PPAR alpha) on the other hand is a ligand-activated transcription factor which gets activated by the endogenous ligand 1-palmitoyl-2-oleoyl-sn-glycerol-3-phosphocholine, and oleylethanolamide (a naturally occurring lipid that regulates satiety), and acts as a key regulator of lipid metabolism. It also acts as a receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. It regulates the peroxisomal beta-oxidation pathway of fatty acids, and also functions as transcription activator for the ACOX1 and P450 genes. We have a variety of PPAR alpha antibodies. I hope you will find this information helpful but please let me know if I can support you with anything else from my end. Thank you very much for choosing Novus Biologicals as your quality reagent supplier and we wish you the best with your research projects.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NB600-636AF350
Alexa Fluor 405 NB600-636AF405
Alexa Fluor 488 NB600-636AF488
Alexa Fluor 532 NB600-636AF532
Alexa Fluor 594 NB600-636AF594
Alexa Fluor 647 NB600-636AF647
Alexa Fluor 700 NB600-636AF700
Alexa Fluor 750 NB600-636AF750
Allophycocyanin NB600-636APC
Biotin NB600-636B
DyLight 350 NB600-636UV
DyLight 405 NB600-636V
DyLight 488 NB600-636G
DyLight 550 NB600-636R
DyLight 594 NB600-636DL594
DyLight 650 NB600-636C
DyLight 680 NB600-636FR
DyLight 755 NB600-636IR
FITC NB600-636F
HRP NB600-636H
Janelia Fluor 549 NB600-636JF549
Janelia Fluor 646 NB600-636JF646
PE NB600-636PE
PerCP NB600-636PCP

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Bioinformatics

Gene Symbol PPARA
Entrez
Uniprot