SPG7 Antibody - BSA Free

Western Blot: SPG7 Antibody - BSA Free [NBP2-93364] - Western blot analysis of various lysates using SPG7 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] - Immunofluorescence analysis of HepG2 cells using SPG7 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] - Immunofluorescence analysis of PC-12 cells using SPG7 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SPG7 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SPG7 Antibody - BSA Free (NBP2-93364) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human SPG7 (NP_003110.1). MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTPTFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRERLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SPG7
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SPG7 Antibody - BSA Free

• CARMGC126331
• cell matrix adhesion regulator
• CMARMGC126332
• EC 3.4.24
• EC 3.4.24.-
• FLJ37308
• paraplegin
• PGNcell adhesion regulator
• spastic paraplegia 7 (pure and complicated autosomal recessive)
• Spastic paraplegia 7 protein
• SPG5C

Background

SPG7 - spastic paraplegia 7, paraplegin (pure and complicated autosomal recessive)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPG7