Cytochrome P450 2B6 Antibody (OTI3D5)

Images

 
Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytochrome P450 2B6 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; ...read more
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytochrome P450 2B6.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human liver tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Cytochrome P450 2B6 ...read more
Western Blot: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 2B6 (Right lane) cDNA for 48 hrs and lysed. ...read more
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human colon tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Staining of paraffin-embedded Human Kidney tissue using anti-Cytochrome P450 2B6 mouse monoclonal antibody.
Flow Cytometry: Cytochrome P450 2B6 Antibody (3D5) [NBP2-01800] - Analysis of Jurkat cells, using anti-Cytochrome P450 2B6 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI3D5
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.1 mg/ml

Order Details

Cytochrome P450 2B6 Antibody (OTI3D5) Summary

Immunogen
Full length human recombinant protein of human CYP2B6(NP_000758) produced in HEK293T cell.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
CYP2B6
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:150
Theoretical MW
56.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-01800.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.1 mg/ml
Purity
Affinity purified

Alternate Names for Cytochrome P450 2B6 Antibody (OTI3D5)

  • CPB6
  • CYP2B
  • CYP2B7
  • CYP2B7P
  • CYPIIB6cytochrome P450, subfamily IIB (phenobarbital-inducible)
  • cytochrome P450 2B6
  • Cytochrome P450 IIB1
  • cytochrome P450, family 2, subfamily B
  • cytochrome P450, family 2, subfamily B, polypeptide 6
  • cytochrome P450, subfamily IIB (phenobarbital-inducible), polypeptide 6
  • EC 1.14.14.1
  • IIB1
  • P450

Background

This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytochrome P450 2B6 Antibody (NBP2-01800)(1)

We have publications tested in 1 confirmed species: Mouse.


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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-70526AF350
Alexa Fluor 405 NBP2-70526AF405
Alexa Fluor 488 NBP2-70526AF488
Alexa Fluor 532 NBP2-70526AF532
Alexa Fluor 594 NBP2-70526AF594
Alexa Fluor 647 NBP2-70526AF647
Alexa Fluor 700 NBP2-70526AF700
Alexa Fluor 750 NBP2-70526AF750
Allophycocyanin NBP2-70526APC
Allophycocyanin/Cy7 NBP2-70526APCCY7
Biotin NBP2-70526B
DyLight 350 NBP2-70526UV
DyLight 405 NBP2-70526V
DyLight 488 NBP2-70526G
DyLight 550 NBP2-70526R
DyLight 594 NBP2-70526DL594
DyLight 650 NBP2-70526C
DyLight 680 NBP2-70526FR
DyLight 755 NBP2-70526IR
FITC NBP2-70526F
HRP NBP2-70526H
Janelia Fluor 549 NBP2-70526JF549
Janelia Fluor 646 NBP2-70526JF646
PE NBP2-70526PE
PE/Atto594 NBP2-70526PEATT594
PE/Cy5.5 NBP2-70526PECY55
PE/Cy7 NBP2-70526PECY7
PerCP NBP2-70526PCP

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Bioinformatics

Gene Symbol CYP2B6
Entrez