| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74327 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74323 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93364-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74323 | |||
![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0006.jpg "Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of extracts (35 ug) from 9 different cell lines by using anti-SPG7 monoclonal antibody.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Immunocytochemistry-Immunofluorescence-NBP2-01860-img0005.jpg)
![Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Flow-Cytometry-NBP2-01860-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Loss of AFG3L2 and/or YME1L is associated with elevated OMA1, diminished SPG7, and a markedly altered pattern of OPA1 protein forms. Whole-cell lysates were resolved using SDS-PAGE and western blotted with antibody to Drp1, Oma1, and Spg7. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/1422-0067/19/12/3930), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0007.jpg)
![Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPG7 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SPG7.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Western-Blot-NBP2-01860-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (OTI1C1) [NBP2-01860] - Immunofluorescent staining of HeLa cells using anti-SPG7 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Immunocytochemistry-Immunofluorescence-NBP2-01860-img0004.jpg)
![Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1C1-Flow-Cytometry-NBP2-01860-img0001.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249415947.jpg "Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941595.jpg)
![Immunohistochemistry-Paraffin: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SPG7 stained by SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:20. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024941493.jpg)
![Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-2120249422671.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: SPG7 Antibody (HL2236) - Azide and BSA Free [NBP3-25694] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (NBP3-25694) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25694_rabbit-spg7-mab-hl2236-azide-and-bsa-free-212024943554.jpg)
![Western Blot: SPG7 Antibody (1G4) [NBP2-45943] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human SPG7(NP_003110) gene (amino acids 300-573) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted w](http://images.novusbio.com/fullsize/SPG7-Antibody-1G4-Western-Blot-NBP2-45943-img0003.jpg "Western Blot: SPG7 Antibody (1G4) [NBP2-45943] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human SPG7(NP_003110) gene (amino acids 300-573) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted w")
![Immunohistochemistry: SPG7 Antibody (1G4) [NBP2-45943] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SPG7-Antibody-1G4-Immunohistochemistry-NBP2-45943-img0002.jpg)
![Immunohistochemistry: SPG7 Antibody (1G4) [NBP2-45943] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SPG7-Antibody-1G4-Immunohistochemistry-NBP2-45943-img0001.jpg)
![Western Blot: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human SPG7(NP_003110) gene (amino acids 300-573) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted w](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1G4-Azide-and-BSA-Free-Western-Blot-NBP2-74323-img0001.jpg "Western Blot: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Negative control E. coli lysate (Left lane) or E. coli lysate containing recombinant protein fragment for human SPG7(NP_003110) gene (amino acids 300-573) (Right lane). Equivalent amounts (5 ug per lane) were separated by SDS-PAGE and then immunoblotted w")
![Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Analysis of Carcinoma of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74323-img0002.jpg)
![Immunohistochemistry: SPG7 Antibody (OTI1G4) - Azide and BSA Free [NBP2-74323] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)](http://images.novusbio.com/fullsize/SPG7-Antibody-OTI1G4-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74323-img0003.jpg)
![Western Blot: SPG7 Antibody - BSA Free [NBP2-93364] - Western blot analysis of various lysates using SPG7 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-93364_rabbit-polyclonal-spg7-antibody-4220251429056.jpg%3Ft=1706682213 "Western Blot: SPG7 Antibody - BSA Free [NBP2-93364] - Western blot analysis of various lysates using SPG7 Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates / proteins: 25 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] - Immunofluorescence analysis of HepG2 cells using SPG7 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93364_rabbit-polyclonal-spg7-antibody-42202514275284.jpg%3Ft=1706682213)
![Immunocytochemistry/ Immunofluorescence: SPG7 Antibody - BSA Free [NBP2-93364] - Immunofluorescence analysis of PC-12 cells using SPG7 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93364_rabbit-polyclonal-spg7-antibody-42202514264467.jpg%3Ft=1706682213)
![Western Blot: SPG7 Overexpression Lysate (Adult Normal) [NBL1-16397] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPG7.](http://images.novusbio.com/fullsize/SPG7-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16397-img0002.jpg "Western Blot: SPG7 Overexpression Lysate (Adult Normal) [NBL1-16397] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SPG7.")
![Western Blot: SPG7 Antibody [H00006687-B01P] - Analysis of SPG7 expression in transfected 293T cell line by SPG7 polyclonal antibody. Lane 1: SPG7 transfected lysate(87.45 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPG7-Antibody-Western-Blot-H00006687-B01P-img0002.jpg "Western Blot: SPG7 Antibody [H00006687-B01P] - Analysis of SPG7 expression in transfected 293T cell line by SPG7 polyclonal antibody. Lane 1: SPG7 transfected lysate(87.45 KDa). Lane 2: Non-transfected lysate.")
![Western Blot: SPG7 Antibody [H00006687-D01P] - Analysis of SPG7 expression in transfected 293T cell line by SPG7 polyclonal antibody.Lane 1: SPG7 transfected lysate(88.20 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/SPG7-Antibody-Western-Blot-H00006687-D01P-img0002.jpg "Western Blot: SPG7 Antibody [H00006687-D01P] - Analysis of SPG7 expression in transfected 293T cell line by SPG7 polyclonal antibody.Lane 1: SPG7 transfected lysate(88.20 KDa).Lane 2: Non-transfected lysate.")