SOD3/EC-SOD Antibody

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Western blot shows lysates of mouse lung and kidney tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Mouse SOD3/EC‑SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

SOD3/EC-SOD Antibody Summary

Immunogen
E. coli-derived recombinant mouse SOD3/EC‑SOD
Ser25-Thr251
Accession # O09164
Specificity
Detects mouse SOD3/EC-SOD in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse SOD1 or SOD2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOD3
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
AF4817 in the following application:

Publications
Read Publications using
AF4817 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOD3/EC-SOD Antibody

  • ECSOD
  • EC-SOD
  • EC-SODEC 1.15.1.1
  • extracellular superoxide dismutase [Cu-Zn]
  • MGC20077
  • SOD3
  • superoxide dismutase 3, extracellular

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Like SOD1, SOD3 binds one Cu 2+ and Zn 2+ ions per subunit but differs in sequence and tissue distribution. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Mouse SOD3 shares ~66% and 82% sequence homology with human and rat SOD3, respectively. SOD3 is a secretory protein and is synthesized with a putative 24-amino acid signal peptide preceding the 227 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SOD3/EC-SOD Antibody (AF4817)(4)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Western Blot
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Review for SOD3/EC-SOD Antibody (AF4817) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF4817:
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WB
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
WB Mouse 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23886589
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23886589

Details

Detection NotesSee PMID 23886589

Comments

CommentsPublished: See PMID 23886589These antibodies work well in Western blot with very low nonspecific band staining

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FAQs for SOD3/EC-SOD Antibody (AF4817) (0)

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Recent Reviews

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Verified Customer
01/08/2015
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol SOD3
Uniprot