Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 30 kDa (as indicated) using 10 ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Glutathione Peroxidase 1/GPX1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL ...read more
Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Glutathione Peroxidase 1
Gly48-Ala201
Accession # P07203
Specificity
Detects endogenous human, mouse, and rat GPX1. In Western blots, this antibody shows no cross-reactivity with recombinant human GPX2 or GPX3.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPX1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
  • Simple Western 10 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
Control
Glutathione Peroxidase 1/GPX1 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF3798 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]

  • Cellular glutathione peroxidase
  • CGPx
  • EC 1.11.1
  • EC 1.11.1.9
  • Glutathione Peroxidase 1
  • GPX1
  • GPx-1
  • GSHPX1
  • GSHPx-1
  • MGC14399
  • MGC88245

Background

Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid, 23 kDa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NB100-1992
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Dr, Gt, Gp, Ha, Pm, Rb, Sh, Sq, Xp
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-76933
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP1-06398
Species: Hu, Rt, Pm
Applications: WB, IB, ICC/IF, IHC, IHC-P
NBP1-86615
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-58917
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-16684
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-32002
Species: Hu, Mu
Applications: WB, IHC, IHC-P, IP
NBP2-20619
Species: Hu, Mu, Rt, Pm
Applications: WB, ICC/IF, IHC, IHC-P
AF5724
Species: Hu, Mu, Rt
Applications: WB, ICC
NBP1-32822
Species: Hu, Mu, Rt, Al, Av, Bv, Ce, Pl
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP, KD
NB200-103
Species: Hu, Mu, Rt, Ch, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-52983
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF3798
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, KO

Publications for Glutathione Peroxidase 1/GPX1 Antibody (AF3798)(14)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(13)
All Applications
Filter By Species
Human
(5)
Mouse
(7)
Rat
(2)
All Species
Showing Publications 1 - 10 of 14. Show All 14 Publications.
Publications using AF3798 Applications Species
MY Lee, A Leonardi, TJ Begley, JA Melendez Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming? Redox Biol, 2019;28(0):101375. 2019 [PMID: 31765888] (Western Blot, Mouse) Western Blot Mouse
G Li, YL Chan, S Sukjamnong, AG Anwer, H Vindin, M Padula, R Zakarya, J George, BG Oliver, S Saad, H Chen A Mitochondrial Specific Antioxidant Reverses Metabolic Dysfunction and Fatty Liver Induced by Maternal Cigarette Smoke in Mice Nutrients, 2019;11(7):. 2019 [PMID: 31330878] (Western Blot, Mouse) Western Blot Mouse
P Youssef, B Chami, J Lim, T Middleton, GT Sutherland, PK Witting Evidence supporting oxidative stress in a moderately affected area of the brain in Alzheimer's disease Sci Rep, 2018;8(1):11553. 2018 [PMID: 30068908] (Human) Human
WL Lau, M Khazaeli, J Savoj, K Manekia, M Bangash, RG Thakurta, A Dang, ND Vaziri, B Singh Dietary tetrahydrocurcumin reduces renal fibrosis and cardiac hypertrophy in 5/6 nephrectomized rats Pharmacol Res Perspect, 2018;6(2):e00385. 2018 [PMID: 29468071] (Western Blot, Rat) Western Blot Rat
V Coleman, P Sa-Nguanmo, J Koenig, TJ Schulz, T Grune, S Klaus, AP Kipp, M Ost Partial involvement of Nrf2 in skeletal muscle mitohormesis as an adaptive response to mitochondrial uncoupling Sci Rep, 2018;8(1):2446. 2018 [PMID: 29402993] (Western Blot, Mouse) Western Blot Mouse
AN Ogawa-Wong, AC Hashimoto, H Ha, MW Pitts, LA Seale, MJ Berry Sexual Dimorphism in the Selenocysteine Lyase Knockout Mouse Nutrients, 2018;10(2):. 2018 [PMID: 29385050] (Western Blot, Mouse) Western Blot Mouse
W Jing, ND Vaziri, A Nunes, Y Suematsu, T Farzaneh, M Khazaeli, H Moradi LCZ696 (Sacubitril/valsartan) ameliorates oxidative stress, inflammation, fibrosis and improves renal function beyond angiotensin receptor blockade in CKD Am J Transl Res, 2017;9(12):5473-5484. 2017 [PMID: 29312499] (Western Blot, Rat) Western Blot Rat
Global survey of cell death mechanisms reveals metabolic regulation of ferroptosis Nat Chem Biol, 2016;0(0):. 2016 [PMID: 27159577] (Western Blot, Human) Western Blot Human
Kurokawa S, Bellinger F, Hill K, Burk R, Berry M Isoform-specific binding of selenoprotein P to the beta-propeller domain of apolipoprotein E receptor 2 mediates selenium supply. J Biol Chem, 2014;289(13):9195-207. 2014 [PMID: 24532792] (Western Blot, Human) Western Blot Human
Byrns, China N, Pitts, Matthew, Gilman, Christy, Hashimoto, Ann C, Berry, Marla J Mice lacking selenoprotein P and selenocysteine lyase exhibit severe neurological dysfunction, neurodegeneration, and audiogenic seizures. J Biol Chem, 2014;289(14):9662-74. 2014 [PMID: 24519931] (Western Blot, Mouse) Western Blot Mouse
Show All 14 Publications.

Reviews for Glutathione Peroxidase 1/GPX1 Antibody (AF3798) (0)

There are no reviews for Glutathione Peroxidase 1/GPX1 Antibody (AF3798). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Glutathione Peroxidase 1/GPX1 Antibody (AF3798) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Additional Glutathione Peroxidase 1/GPX1 Products

Bioinformatics Tool for Glutathione Peroxidase 1/GPX1 Antibody (AF3798)

Discover related pathways, diseases and genes to Glutathione Peroxidase 1/GPX1 Antibody (AF3798). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Glutathione Peroxidase 1/GPX1 Antibody (AF3798)

Discover more about diseases related to Glutathione Peroxidase 1/GPX1 Antibody (AF3798).
 

Pathways for Glutathione Peroxidase 1/GPX1 Antibody (AF3798)

View related products by pathway.

PTMs for Glutathione Peroxidase 1/GPX1 Antibody (AF3798)

Learn more about PTMs related to Glutathione Peroxidase 1/GPX1 Antibody (AF3798).

Blogs on Glutathione Peroxidase 1/GPX1

There are no specific blogs for Glutathione Peroxidase 1/GPX1, but you can read our latest blog posts.
Coronavirus Brochure

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol GPX1
Uniprot