Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]

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Glutathione Peroxidase 1/GPX1 was detected in perfusion fixed frozen sections of rat liver using 15 µg/mL Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1/GPX1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Western blot shows lysates of human, mouse and rat liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Glutathione Peroxidase 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
Simple Western lane view shows lysates of human liver tissue and mouse liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Glutathione Peroxidase 1/GPX1 at approximately 30 kDa (as indicated) using 10 ...read more
Genetic Strategies: Western blot shows lysates of HEK293T human embryonic kidney parental cell line and Glutathione Peroxidase 1/GPX1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL ...read more
Induction of oxidative stress defense during muscle mild mitochondrial stress. (A) Skeletal muscle mRNA expression in quadriceps muscle of oxidative stress defense markers of 24 wks old animals (n = 8–10). (B and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, IHC, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price

Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Glutathione Peroxidase 1
Gly48-Ala201
Accession # P07203
Specificity
Detects endogenous human, mouse, and rat GPX1. In Western blots, this antibody shows no cross-reactivity with recombinant human GPX2 or GPX3.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPX1
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3798 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Glutathione Peroxidase 1/GPX1 Antibody [Unconjugated]

  • Cellular glutathione peroxidase
  • CGPx
  • EC 1.11.1
  • EC 1.11.1.9
  • Glutathione Peroxidase 1
  • GPX1
  • GPx-1
  • GSHPX1
  • GSHPx-1
  • MGC14399
  • MGC88245

Background

Glutathione Peroxidase 1 (GPX1), also known as Cellular Glutathione Peroxidase, is a 201 amino acid , 23 kDa member of the glutathione peroxidase antioxidant enzyme family. The Glutathione Peroxidase family protects cell surfaces, extracellular fluid components, and enzymes from oxidative stress by catalysing the reduction of hydrogen peroxide, lipid peroxides, and organic hydroperoxide using reduced glutathione. GPX1 is a 92 kDa homotetramer consisting of four identical 23 kDa subunits, each containing a selenocysteine residue at the active site. GPX1 is an important antioxidant enzyme and protects red blood cells and other cells from oxidative damage. Deficiencies in red cell GPX1 have been associated with hemolytic anemia. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Glutathione Peroxidase 1/GPX1 Antibody (AF3798)(27)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(14)
All Applications
Filter By Species
Human
(5)
Mouse
(8)
Rat
(2)
All Species
Showing Publications 1 - 10 of 27. Show All 27 Publications.
Publications using AF3798 Applications Species
Pamela Toh, Lucia A. Seale, Marla J. Berry, Daniel J. Torres Prolonged maternal exposure to glucocorticoids alters selenoprotein expression in the developing brain Frontiers in Molecular Neuroscience 2023-03-24 [PMID: 37033382]
Sara Evke, Qishan Lin, Juan Andres Melendez, Thomas John Begley Epitranscriptomic Reprogramming Is Required to Prevent Stress and Damage from Acetaminophen Genes (Basel) 2022-02-25 [PMID: 35327975]
Victor W. Kilonzo, Alexandru R. Sasuclark, Daniel J. Torres, Celine Coyle, Jennifer M. Pilat, Christopher S. Williams et al. Juvenile Selenium Deficiency Impairs Cognition, Sensorimotor Gating, and Energy Homeostasis in Mice Frontiers in Nutrition 2021-05-07 [PMID: 34026810]
Li Zhang, Bei Zhang, Linjing Wang, Mingwu Lou, Yunxia Shen Huanglian‑Houpo drug combination ameliorates H1N1‑induced mouse pneumonia via cytokines, antioxidant factors and TLR/MyD88/NF‑ kappa B signaling pathways Experimental and Therapeutic Medicine 2021-02-26 [PMID: 33747167]
Vuong Cat Khanh, Toshiharu Yamashita, Kinuko Ohneda, Chiho Tokunaga, Hideyuki Kato, Motoo Osaka et al. Rejuvenation of mesenchymal stem cells by extracellular vesicles inhibits the elevation of reactive oxygen species Scientific Reports 2020-10-14 [PMID: 33057147]
Y Chu, RS Lan, R Huang, H Feng, R Kumar, S Dayal, KS Chan, DF Dai Glutathione peroxidase-1 overexpression reduces oxidative stress, and improves pathology and proteome remodeling in the kidneys of old mice Aging Cell, 2020-05-13;0(0):. 2020-05-13 [PMID: 32400101] (Western Blot, Mouse) Western Blot Mouse
Andrea Leonardi, Nataliia Kovalchuk, Lei Yin, Lauren Endres, Sara Evke, Steven Nevins et al. The epitranscriptomic writer ALKBH8 drives tolerance and protects mouse lungs from the environmental pollutant naphthalene Epigenetics 2020-10-02 [PMID: 32303148]
MY Lee, A Leonardi, TJ Begley, JA Melendez Loss of epitranscriptomic control of selenocysteine utilization engages senescence and mitochondrial reprogramming? Redox Biol, 2019-11-11;28(0):101375. 2019-11-11 [PMID: 31765888] (Western Blot, Mouse) Western Blot Mouse
G Li, YL Chan, S Sukjamnong, AG Anwer, H Vindin, M Padula, R Zakarya, J George, BG Oliver, S Saad, H Chen A Mitochondrial Specific Antioxidant Reverses Metabolic Dysfunction and Fatty Liver Induced by Maternal Cigarette Smoke in Mice Nutrients, 2019-07-21;11(7):. 2019-07-21 [PMID: 31330878] (Western Blot, Mouse) Western Blot Mouse
Patrick M. Cowley, Guanying Wang, Philip M. Swigart, Anaha Raghunathan, Nikitha Reddy, Pranavi Dulam et al. Reversal of right ventricular failure by chronic alpha 1A-subtype adrenergic agonist therapy American Journal of Physiology-Heart and Circulatory Physiology 2019-01-01 [PMID: 30412439]
Show All 27 Publications.

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Bioinformatics

Gene Symbol GPX1
Uniprot