SOD3/EC-SOD Antibody [Alexa Fluor® 750]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 750

SOD3/EC-SOD Antibody [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant mouse SOD3/EC‑SOD
  Ser25-Thr251
  Accession # O09164
• Specificity: Detects mouse SOD3/EC‑SOD in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse SOD1 or SOD2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SOD3/EC-SOD Antibody [Alexa Fluor® 750]

• ECSOD
• EC-SOD
• EC-SODEC 1.15.1.1
• extracellular superoxide dismutase [Cu-Zn]
• MGC20077
• SOD3
• superoxide dismutase 3, extracellular

Background

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Like SOD1, SOD3 binds one Cu²⁺ and Zn²⁺ ions per subunit but differs in sequence and tissue distribution. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Mouse SOD3 shares ~66% and 82% sequence homology with human and rat SOD3, respectively. SOD3 is a secretory protein and is synthesized with a putative 24-amino acid signal peptide preceding the 227 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.