SOD1/Cu-Zn SOD Antibody

Images

 
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody.
Immunocytochemistry/ Immunofluorescence: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOD1 protein stained by SOD1 antibody (NBP1-31204) diluted at ...read more
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Paraffin-embedded Muscle, using antibody at 1:500 dilution. (Mouse)
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Sample (50 ug of whole cell lysate) A: Rat brain 15% SDS PAGE; antibody diluted at 1:1000.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Analysis of SOD1 and beta-actin (lower panel) Sample (30 ug of whole cell lysate) A: HeLa mock control B: HeLa transfected shSOD1 15% SDS PAGE.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Sample(30 ug whole cell lysate) A:NT2D1 B:PC-3 C:U87-MG D:SK-N-SH 15% SDS PAGE, antibody dilution 1:500.
Western Blot: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody diluted by 1:1000.
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Paraffin-embedded Colon ca, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SOD1/Cu-Zn SOD Antibody [NBP1-31204] - Paraffin-embedded rat brain, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.47 mg/ml
     

Genetic Strategies

   

Order Details

SOD1/Cu-Zn SOD Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SOD1. The exact sequence is proprietary.
Localization
Cytoplasm
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SOD1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:10000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-31204 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.47 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOD1/Cu-Zn SOD Antibody

  • ALS
  • ALS1
  • amyotrophic lateral sclerosis 1 (adult)
  • Cu
  • Cu/Zn superoxide dismutase
  • CuZn SOD
  • Cu-Zn SOD
  • EC 1.15.1.1
  • homodimer
  • hSod1
  • indophenoloxidase A
  • Ipo1
  • IPOA
  • SOD
  • SOD, cytosolic
  • SOD, Soluble
  • SOD1
  • superoxide dismutase [Cu-Zn]
  • Superoxide dismutase 1
  • superoxide dismutase 1, soluble
  • Zn superoxide dismutase, EC 1.15.1.110superoxide dismutase, cystolic

Background

Superoxide dismutase (SOD) is an antioxidant enzyme involved in the defense system against reactive oxygen species (ROS). SOD catalyzes the dismutation reaction of superoxide radical anion (O2-) to hydrogen peroxide, which is then catalyzed to innocuous O2 and H2O by glutathione peroxidase and catalase. Several classes of SOD have been identified. These include intracellular copper, zinc SOD (Cu, Zn-SOD/SOD1), mitochondrial manganese SOD (Mn-SOD/SOD2) and extracellular Cu, Zn-SOD (EC-SOD/SOD3). SOD1 is found in all eukaryotic species as a homodimeric 32-kDa enzyme containing one each of Cu and Zn ion per subunit. The manganese containing 80-kDa tetrameric enzyme SOD2 is located in the mitochondrial matrix in close proximity to a primary endogenous source of superoxide, the mitochondrial respiratory chain. SOD3 is a heparin binding multimer of disulfide linked dimers, primarily expressed in human lungs, vessel walls and airways. SOD4 is a copper chaperone for superoxide dismutase(CCS), which specifically delivers Cu to copper/zinc superoxide dismutase. CCS may activate copper/zinc superoxide dismutase through direct insertion of the Cu cofactor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for SOD1/Cu-Zn SOD Antibody (NBP1-31204) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Pig.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31204:
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WB
(1)
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Images Ratings Applications Species Date Details
Western Blot SOD1/Cu-Zn SOD NBP1-31204
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reviewed by:
Anonymous
WB Pig 11/17/2016
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Summary

ApplicationWestern Blot
Sample TestedOvary tissue
SpeciesPig

Comments

CommentsSample Information:
     Treatment: None. Run on control tissue
Controls:
     Positive Control: None
     Negative Control: Primary only, secondary only, and secondary with IgG all negative
     Loading Control: Ponceau S stain

Total Protein Loaded: 40 ug/well

Electrophoresis:
Gel Percentage: Gradient, 4-20%
Electrophoresis Conditions:
     Voltage: 50V then 90V
     Time: 5 min then 1 hr

Membrane Transfer:
     Method (Submersion/Semi-dry): Semi-dry using iBlot2 system
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 7 min (iBlot2 Protocol 0)

Blocking:
     Blocking Solution: 5% BSA in.2% PBST
     Time: 1.5 hours

Primary Antibody:
     Dilution: 1:1000
     Diluent Buffer: 5% BSA in.2% PBST     
     Incubation Time: overnight     
     Incubation Temperature: 4°C

Washing Conditions:
     Wash Solution:.2% PBST
     Time and Repetitions: 3X, 10 minutes each

Secondary Antibody
Manufacturer and Catalog #: Cell Signaling, #7074
Secondary description: goat anti-rabbit
     Dilution: 1:1000
     Diluent Buffer: 5% BSA in.2% PBST
     Incubation Time: 1 hour
     Incubation Temperature: room temperature

Detection Method:
Detection: SignalFire ECL reagent (Cell Signaling)
Procedure: ECL on blot 3 min
Development Time: 5 min

Molecular weight of band(s): 13 kDa

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SOD1/Cu-Zn SOD Antibody (NBP1-31204) (0)

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Secondary Antibodies

 

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Recent Reviews

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2
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1
0

Anonymous
11/17/2016
Application: WB
Species: Pig

Bioinformatics

Gene Symbol SOD1
Entrez