Orthogonal Strategies: Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Analysis in human cerebral cortex and liver tissues. Corresponding NSF RNA-seq data are presented for the same tissues.
Western Blot: NSF Antibody [NBP1-87035] - Analysis in human cell line A-549.
Immunocytochemistry/ Immunofluorescence: NSF Antibody [NBP1-87035] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human testis shows moderate to strong cytoplasmic postivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows low expression as expected.
Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry-Paraffin: NSF Antibody [NBP1-87035] - Staining of human liver shows no positivity in hepatocytes as expected.
Rat reactivity reported in scientific literature (PMID: 24443234).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for NSF Antibody
NEM-sensitive fusion protein
N-ethylmaleimide-sensitive factor-like protein
N-ethylmaleimide-sensitive fusion protein
Vesicular-fusion protein NSF
N ethylmaleimide sensitive factor (NSF) is an ~83 kDa protein (predicted MW) which was initially isolated based on its ability to restore intercisternal Golgi transport to N ethylmaleimide treated membranes. NSF was subsequently discovered to be involved in a variety of membrane fusion events. NSF is an ATPase with two ATP binding domains that are essential for membrane fusion and homo oligomerization. In order to interact with target membranes, NSF requires another set of soluble proteins called Soluble NSF attachment proteins (SNAPs). NSF has been shown to play a prominent role in synaptic vesicle fusion. Together with synaptotagmin and a SNAP, NSF modulates the interaction of SNAP25, VAMP, and syntaxin, in an ATP dependent manner, to form a complex with a sedimentation coefficient of 20 S. This complex may represent an intermediate involved in synaptic vesicle docking and fusion.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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