VAMP3/Cellubrevin Antibody

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of 25 ug of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates.

Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody (left).

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of recombinant rat VAMP-3.

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP-3 in hela cell extract.

Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of HeLa cell lysates.

Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of Neuro-2a cells (right) compared to a negative control without primary antibody (left).

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

VAMP3/Cellubrevin Antibody Summary

• Immunogen: Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: VAMP3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:100
  • Western Blot 1:1000 - 1:5000
• Application Notes: May be useful in WB. WB: Detects a 13 kDa protein (predicted MW 12 kDa). ICC/IF usage was reported in scientific literature.
• Control:

  VAMP3/Cellubrevin Overexpression Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 1 mg/ml BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for VAMP3/Cellubrevin Antibody

• CEBCellubrevin
• cellubrevin
• SYB3
• Synaptobrevin-3
• VAMP-3
• vesicle-associated membrane protein 3 (cellubrevin)
• vesicle-associated membrane protein 3

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, all VAMP isoform levels appear to be significantly reduced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for VAMP3/Cellubrevin Antibody (NB300-510)(11)

Publications using NB300-510

• Fekete CD, Horning RZ, Doron MS, Nishiyama A Cleavage of VAMP2/3 affects oligodendrocyte lineage development in the developing mouse spinal cord The Journal of neuroscience : the official journal of the Society for Neuroscience 2023-08-23 [PMID: 37620160]
• Calcagni' A, Staiano L, Zampelli N et al. Loss of the batten disease protein CLN3 leads to mis-trafficking of M6PR and defective autophagic-lysosomal reformation Nature communications 2023-07-03 [PMID: 37400440] (WB)
• L Pan, A Trimarco, AJ Zhang, K Fujimori, Y Urade, LO Sun, C Taveggia, Y Zhang Oligodendrocyte-lineage cell exocytosis and L-type prostaglandin D synthase promote oligodendrocyte development and myelination Elife, 2023-02-13;12(0):. 2023-02-13 [PMID: 36779701]
• Gu Y, Princely Y, Kumar S et al. Mammalian Atg8 proteins regulate lysosome and autolysosome biogenesis through SNAREs. EMBO J. 2019-10-18 [PMID: 31625181]
• Beaton N, Rudigier C, Moest H et al. TUSC5 regulates insulin-mediated adipose tissue glucose uptake by modulation of GLUT4 recycling. Mol Metab 2015-11-01 [PMID: 26629404] (ICC/IF)
• Vogel GF, Klee KM, Janecke AR et al. Cargo-selective apical exocytosis in epithelial cells is conducted by Myo5B, Slp4a, Vamp7, and Syntaxin 3. J Cell Biol. 2015-11-09 [PMID: 26553929] (WB, Human)
• Hager HA, Roberts RJ, Cross EE et al. Identification of a novel Bves function: regulation of vesicular transport. EMBO J 2010-02-03 [PMID: 20057356] (ICC/IF, Canine)
• Williams D, Pessin JE. Mapping of R-SNARE function at distinct intracellular GLUT4 trafficking steps in adipocytes. J Cell Biol, 2008;180(2):375-87. 2008-01-28 [PMID: 18227281]
• Han J, Yoon S, Park H Vamp3-dependent secretion of endocytic BDNF from astrocytes Research Square 2021-08-04 (ICC/IF, WB, Mouse)
• Han J, Yoon S, Park H. Endocytic BDNF secretion regulated by Vamp3 in astrocytes Scientific reports 2021-10-27 [PMID: 34707216] (ICC/IF, Human)
• Tiwari N, Wang C-C, Brochetta C et al. VAMP-8 segregates mast cell-preformed mediator exocytosis from cytokine trafficking pathways. Blood111(7):3665-74. 2008-04-01 [PMID: 18203950]

Bioinformatics

• Gene Symbol: VAMP3
• Entrez:
  • 9341(Human)
  • 22319(Mouse)
• Uniprot:
  • P63024(Mouse)