VAMP3/Cellubrevin Antibody

Images

 
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of 25 ug of mouse brain (lane 1), rat brain (lane 2) and human brain (lane 3) lysates.
Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of C6 cells (right) compared to a negative control without primary antibody ...read more
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of recombinant rat VAMP-3.
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP-3 in hela cell extract.
Western Blot: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of HeLa cell lysates.
Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary ...read more
Immunocytochemistry/ Immunofluorescence: VAMP3/Cellubrevin Antibody [NB300-510] - Analysis of VAMP3 (green) showing staining in the cytoplasm of Neuro-2a cells (right) compared to a negative control without primary ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

VAMP3/Cellubrevin Antibody Summary

Immunogen
Sythetic peptide corresponding to residues M(1) S T G V P S G S S A A T G S N R R(18) C of mouse VAMP-3.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
VAMP3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:100
Application Notes
May be useful in WB. WB: Detects a 13 kDa protein (predicted MW 12 kDa). ICC/IF usage was reported in scientific literature.
Control
VAMP3/Cellubrevin Lysate (NBL1-17688)
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB300-510 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for VAMP3/Cellubrevin Antibody

  • CEBCellubrevin
  • cellubrevin
  • SYB3
  • Synaptobrevin-3
  • VAMP-3
  • vesicle-associated membrane protein 3 (cellubrevin)
  • vesicle-associated membrane protein 3

Background

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, all VAMP isoform levels appear to be significantly reduced.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for VAMP3/Cellubrevin Antibody (NB300-510)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol VAMP3
Entrez
Uniprot