SNAP25 Antibodies

HAF016). A specific band was detected for SNAP25 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF016). A specific band was detected for SNAP25 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of A172 human glioblastoma cell line, H4 human neuroglioma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat SNAP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5946) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for SNAP25 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

NL010). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counterstained with DAPI (blue). SNAP25 immunoreactivity was localized to the synaptic termini. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL010). Actin filaments were stained with FITC-conjugated Phalloidin (green) and cell nuclei were counterstained with DAPI (blue). SNAP25 immunoreactivity was localized to the synaptic termini. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

556-NG) prior to immersion fixation. SNAP25 was detected using a Sheep Anti-Human/Mouse/Rat SNAP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5946). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Sheep Affinity-purified IgG Secondary Antibody (red; NL010). The cell nuclei were counterstained with DAPI (blue). SNAP25 immunoreactivity was localized to the synaptic termini. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> 556-NG) prior to immersion fixation. SNAP25 was detected using a Sheep Anti-Human/Mouse/Rat SNAP25 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5946). The cells were stained with the NorthernLights 557-conjugated Donkey Anti-Sheep Affinity-purified IgG Secondary Antibody (red; NL010). The cell nuclei were counterstained with DAPI (blue). SNAP25 immunoreactivity was localized to the synaptic termini. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_lightbox"> HAF016). This experiment was conducted under reducing conditions and using the 2-40 kDa separation system." class="big_thumb" />

HAF018). A specific band was detected for SNAP25 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for SNAP25 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of human cerebellum tissue. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human SNAP25 Monoclonal Antibody (Catalog # MAB11729) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SNAP25 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synapses. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synapses. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the vesicles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) and counterstained with hematoxylin (blue). Specific staining was localized to the vesicles. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="SNAP25 was detected in immersion fixed A172 human glioblastoma cells (positive) and absent in HL‑60 human acute promyelocytic leukemia cells (negative) using Mouse Anti-Human SNAP25 Monoclonal Antibody (Catalog # MAB11418) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />