| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74245 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5946-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-81057-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB600-692-0.025mg | |||
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody [NBP1-33714] - SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP25 protein stained by SNAP25 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33714-img0012.jpg "Immunocytochemistry/Immunofluorescence: SNAP25 Antibody [NBP1-33714] - SNAP25 antibody detects SNAP25 protein at cell body and synaptic vesicles by immunofluorescent analysis. Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: SNAP25 protein stained by SNAP25 antibody diluted at 1:500. Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.")
![Immunohistochemistry-Frozen: SNAP25 Antibody [NBP1-33714] - Frozen sectioned E13.5 Rat brain. Green: SNAP25 protein stained by SNAP25 antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [11710] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Frozen-NBP1-33714-img0011.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Mouse fore brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-33714] - Rat hind brain. SNAP25 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-33714-img0010.jpg)
![Immunohistochemistry-Whole-Mount: SNAP25 Antibody [NBP1-33714] - Paraformaldehyde-fixed 2 days-post-fertilization zebrafish embryo. Green: SNAP25 stained by SNAP25 antibody diluted at 1:50.Antigen Retrieval: Tris-HCl buffer, pH 9.0, 20 min at 70 C](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-immunohistochemistry-whole-mount-127202384126.jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] -Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-12720238431..jpg)
![Western Blot: SNAP25 Antibody [NBP1-33714] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAP25 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/antibody/nbp1-33714_rabbit-polyclonal-snap25-antibody-western-blot-127202384456..jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0024.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Analysis in human cerebral cortex and testis tissues using NBP1-88769 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in human cell lines U2OS and MCF-7 using anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0017.jpg)
![Western Blot: SNAP25 Antibody [NBP1-88769] - Analysis in mouse cerebral cortex tissue.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NBP1-88769-img0025.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebral cortex shows strong positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0020.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human cerebellum shows strong cytoplasmic positivity in neuropil.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0021.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human lymph node shows no positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0022.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody [NBP1-88769] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Immunohistochemistry-Paraffin-NBP1-88769-img0023.jpg)
![Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Western-Blot-NBP2-00795-img0002.jpg "Western Blot: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SNAP25 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SNAP25.")
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4G5) [NBP2-00795] Staining of COS7 cells transiently transfected by pCMV6-ENTRY SNAP25.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunocytochemistry-Immunofluorescence-NBP2-00795-img0009.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human liver tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0008.jpg)
![Flow Cytometry: SNAP25 Antibody (4G5) [NBP2-00795] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SNAP25 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Flow-Cytometry-NBP2-00795-img0001.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0003.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0004.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0005.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0006.jpg)
![Immunohistochemistry-Paraffin: SNAP25 Antibody (4G5) [NBP2-00795] - Staining of paraffin-embedded Human colon tissue using anti-SNAP25 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4G5-Immunohistochemistry-Paraffin-NBP2-00795-img0007.jpg)
![Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-288202412493458.jpg "Western Blot: SNAP25 Antibody (JE45-86) [NBP3-32961] - Western blot analysis of SNAP25 on different lysates with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/2,000 dilution. Lane 1: SH-SY5Y cell lysate (20 ug/Lane) Lane 2: Neuro-2a cell lysate (20 ug/Lane) Lane 3: K-562 cell lysate (negative) (20 ug/Lane) Lane 4: Human brain tissue lysate (40 ug/Lane) Predicted band size: 23 kDa Observed band size: 25 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32961) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32961) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-298202414161869.jpg)
![Immunocytochemistry/ Immunofluorescence: SNAP25 Antibody (JE45-86) [NBP3-32961] - Immunocytochemistry analysis of SH-SY5Y cells labeling SNAP25 with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SNAP25 antibody (NBP3-32961) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202410484855.jpg)
![Flow Cytometry: SNAP25 Antibody (JE45-86) [NBP3-32961] - Flow cytometric analysis of SH-SY5Y cells labeling SNAP25. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32961, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32961_rabbit-snap25-mab-je45-86-29202416372172.jpg)
![Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.](http://images.novusbio.com/fullsize/SNAP25-Antibody-Western-Blot-NB100-1492-img0011.jpg "Western Blot: SNAP25 Antibody [NB100-1492] - (0.005ug/ml) staining of Human Brain (Cerebellum) (A), Mouse Brain (B) and Rat Brain (C) lysates (35ug protein in RIPA buffer). Detected by chemiluminescence.")
![Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Western-Blot-NBP1-04344-img0002.jpg "Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.")
![Immunocytochemistry/Immunofluorescence: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Immunocytochemistry-Immunofluorescence-NBP1-04344-img0009.jpg)
![Flow Cytometry: SNAP25 Antibody (4E11) [NBP1-04344] - Analysis of SNAP25 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Flow-Cytometry-NBP1-04344-img0006.jpg)
![Western Blot: SNAP25 Antibody (4E11) [NBP1-04344] - The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/SNAP25-Antibody-4E11-Western-Blot-NBP1-04344-img0005.jpg)
![Western Blot: SNAP25 Antibody (SP12) [NB600-692] - Human cell line mix using a 1:1000 dilution.](http://images.novusbio.com/fullsize/SNAP25-Antibody-SP12-Western-Blot-NB600-692-img0006.jpg "Western Blot: SNAP25 Antibody (SP12) [NB600-692] - Human cell line mix using a 1:1000 dilution.")
![Immunohistochemistry-Paraffin: SNAP25 Antibody (SP12) [NB600-692] - Human Cerebellar Granular Neurons stained with Mouse anti Human SNAP-25](http://images.novusbio.com/fullsize/SNAP25-Antibody-SP12-Immunohistochemistry-Paraffin-NB600-692-img0001.jpg)
![Western Blot: SNAP25 Antibody (S01-1C6) [NBP3-19776] - Western blot detection of SNAP25 in Rat Brain lysates using NBP3-19776 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 25kDa.](http://images.novusbio.com/fullsize/SNAP25-Antibody-S01-1C6-Western-Blot-NBP3-19776-img0001.jpg "Western Blot: SNAP25 Antibody (S01-1C6) [NBP3-19776] - Western blot detection of SNAP25 in Rat Brain lysates using NBP3-19776 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 25kDa.")