NAPA Antibody

Images

 
Western Blot: NAPA Antibody [NBP1-32493] - Various tissue extracts (50 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with alpha SNAP antibody [N2C3] diluted at 1:5000. The HRP-conjugated anti-rabbit ...read more
Immunocytochemistry/ Immunofluorescence: NAPA Antibody [NBP1-32493] - DIV10 rat E18 primary hippocampal neurons were fixed in 4% paraformaldehyde at RT for 15 min. Green: alpha SNAP protein stained by alpha SNAP ...read more
Immunohistochemistry-Paraffin: NAPA Antibody [NBP1-32493] - Paraffin-embedded mouse lung. alpha SNAP antibody [N2C3] ) dilution: 1:500.
Western Blot: NAPA Antibody [NBP1-32493] - NAPA Antibody Sample(30 ug whole cell lysate)A:Hep G2 10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: NAPA Antibody [NBP1-32493] - NAPA Antibody Analysis of methanol-fixed A431, using antibody at 1:200 dilution.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Pm, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NAPA Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human alpha SNAP. The exact sequence is proprietary.
Localization
Membrane, Peripheral membrane protein
Predicted Species
Porcine (97%), Bovine (98%), Rhesus Macaque (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAPA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1:500-1:10000
Application Notes
Immunoprecipitation Assay-dependent dilution
Theoretical MW
33 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (89%),Chimpanzee (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for NAPA Antibody

  • alpha-SNAP
  • alpha-soluble NSF attachment protein
  • N-ethylmaleimide-sensitive factor attachment protein alpha
  • N-ethylmaleimide-sensitive factor attachment protein, alpha
  • S
  • SNAPA
  • SNAP-alpha

Background

NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues. Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Product General Protocols

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol NAPA