Western Blot: Snail Antibody [NBP2-27293] - Cisplatin-resistant gastric cancer cells exhibit an EMT-like phenotypic change. Epithelial marker E-cadherin, ZO1 and EMT-related transcription factor Snail were examined by ...read more
Immunohistochemistry-Paraffin: Snail Antibody [NBP2-27293] - Analysis of in FFPE human kidney tissue using this antibody at 5 ug/mL.
Western Blot: Snail Antibody [NBP2-27293] - HER2 is overexpressed and both Herceptin incubation and siRNAs against HER2 reversed the EMT in cisplatin-resistant gastric cancer cells. Effects of Herceptin on levels of ZO1 ...read more
Simple Western: Snail Antibody [NBP2-27293] - Image shows a specific band for SNAIL in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size. Use in Western blot reported in scientific literature (PMID 28260923). Use in Immunohistochemistry reported in scientific literature (PMID: 30488343).
Read Publications using NBP2-27293 in the following applications:
The amino acid sequence used as immunogen is 61% homologous with human SNAI2 protein.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein A purified
Alternate Names for Snail Antibody
Protein snail homolog 1
snail 1 homolog
snail 1 zinc finger protein
snail family zinc finger 1
snail homolog 1
zinc finger protein SNAI1
Zinc finger protein SNAI1 (SNAIL) is a zinc finger transcriptional repressor which down regulates the expression of ectodermal gene within the mesoderm and thus functions to form the mesoderm and neural crest. It is reported that SNAI1 acts as an important factor of tumor invasion as evidenced by its role in E-cadherin down-regulation and induction of epithelial-mesenchymal transition (EMT). In human breast cancer, the expression of SNAI1 and/or the homologous SNAI2 (Slug) has been associated with E-cadherin repression, local or distant metastasis, tumor recurrence, or poor prognosis in different tumor series. It is also reported that Snail 1 protein in the stroma may be a new putative prognosis marker for colon tumors. In recent studies SNAI1 is found to be potential target for proteasome degradation by hypoxia-controlled FBXL14 ubiquitin ligase targets.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for Snail Antibody (NBP2-27293). (Showing 1 - 2 of 2 FAQs).
There are many kinds of antibodies you supply against SNAIL. Could you give me your recommendation? Which is the best for the IHC-P experiments the species of my samples to be tested is human.
Our SNAIL antibody with catalog # NBP1-19529 has been successfully validated for IHC-P in paraffin-embedded human lung carcinoma tissue and I would highly recommend you to use the same for your samples too. The working dilutions of this antibody for IHC-P ranges from 1:50 - 1:200 and beside IHC, you can use this antibody for Western Blot and Immunofluorescence also.
Could you please let me know the secondary antibody recommended for this particular primary antibody (Catalog NumberNBP2-27293) so that I could order both primary & secondary antibodies.
Our SNAIL antibody with catalogue number NBP2-27293 is a rabbit polyclonal, and as such you would require an anti-rabbit secondary to detect it. You can see our full range of anti-rabbit secondary antibodies at the following link, and by using the search filters at the left hand side of the webpage you can select a suitably conjugated product for your research: View Secondary Antibodies
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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation
Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.