Snail Antibody - BSA Free

Western Blot: Snail Antibody [NBP2-27293] - Cisplatin-resistant gastric cancer cells exhibit an EMT-like phenotypic change. Epithelial marker E-cadherin, ZO1 and EMT-related transcription factor Snail were examined by Western blot. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep20502), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Snail Antibody [NBP2-27293] - Analysis of a FFPE tissue section of mouse placenta using 1:200 dilution of SNAIL antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

Western Blot: Snail Antibody [NBP2-27293] - HER2 is overexpressed and both Herceptin incubation and siRNAs against HER2 reversed the EMT in cisplatin-resistant gastric cancer cells. Effects of Herceptin on levels of ZO1 and Snail. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep20502), licensed under a CC-BY license.

Immunohistochemistry-Paraffin: Snail Antibody [NBP2-27293] - Analysis of in FFPE human kidney tissue using this antibody at 5 ug/mL.

Simple Western: Snail Antibody [NBP2-27293] - Image shows a specific band for SNAIL in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Western Blot: Snail Antibody - BSA Free [NBP2-27293] - SiRNAs against HER2 could abrogate the EMT phenotype & cell invasiveness in cisplatin-resistant gastric cancer cells.(A) Validation of silencing efficiency of HER2. Cells were transiently transfected with 50 nM HER2-specific siRNAs #1-#3 or a control siRNA for 48 h. (B) Knock-down of HER2 abrogated EMT morphology. Cells were transiently transfected with 50 nM specific siRNAs targeting HER2 for 48 h, Representative images of cellular morphology were shown. (C) Knock-down of HER2 abolished EMT phenotype. Cells were treated as in Fig. 4B. FITC-phalloidin staining of F-actin were performed & representative images were shown. (D) Effects of HER2 knock-down on the levels of ZO1 & Snail. (E) Effects of HER2 knock-down on cell migration. Representative images of transwell cell migration assay were shown. (F) Summary of Fig. 4E. Values represented the mean ± SD from three independent experiments with triplicate samples. *P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26846307), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Snail Antibody - BSA Free [NBP2-27293] - HER2 is overexpressed & both Herceptin incubation & siRNAs against HER2 reversed the EMT in cisplatin-resistant gastric cancer cells.(A) Western blot of HER2 in MGC803/DDP & AGS/DDP cell lines, & parental MGC803 & AGS cells. GAPDH was used as loading control. (B) Relative amplification of HER2 in parental & cisplatin-resistant cells. Results were presented as the mean ± SD of two independent experiments, *P < 0.05 compared with parental cells. (C) Herceptin incubation abrogated EMT morphology in MGC803/DDP cells. Representative images of cellular morphology of MGC803/DDP cells, plus parental MGC803 cells treated with Herceptin for 24 h were shown. Hercep represents Herceptin. (D) Herceptin incubation reversed EMT morphology in MGC803/DDP cells. Representative images of FITC-phalloidin staining of F-actin after 100 μg/ml Herceptin treatment for 24 h were shown. (E) Effects of Herceptin on levels of ZO1 & Snail. (F) CP724714 treatment abolished EMT-like cell morphology. Cells were incubated with 10 μM CP724714 for 24 h. Representative images of cellular morphology of were captured. (G) CP724714 treatment abrogated EMT-like cell morphology. Cells were treated as in Fig. 4F, FITC-phalloidin staining of F-actin was performed & typical images were shown. (H) Effects of CP724714 on ZO1 & Snail. (I) CP724714 inhibited the increased cell migration in cisplatin-resistant gastric cancer cells. Representative images were presented here. (J) CP724714 inhibited the increased cell migration in cisplatin-resistant gastric cancer cells. Values represented the mean ± SD from three independent experiments with triplicate samples. *P < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26846307), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Snail Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Snail Antibody - BSA Free (NBP2-27293) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-Snail Antibody: Cited in 14 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 1-60 of human SNAI1 was used as the immunogen.
• Predicted Species: Rat (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SNAI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200. Use reported in scientific literature (PMID 30488343)
  • Immunohistochemistry-Paraffin 1:200
  • Simple Western 1:500
  • Western Blot 1 - 2 ug/ml. Use reported in scientific literature (PMID 28260923)
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in 12Z, Ovaries; separated by Size; antibody dilution of 1:25

Reactivity Notes

The amino acid sequence used as immunogen is 61% homologous with human SNAI2 protein.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Snail Antibody - BSA Free

• dJ710H13.1
• Protein sna
• Protein snail homolog 1
• SLUGH2
• SNA
• SNAH
• SNAI1
• snail 1 homolog
• snail 1 zinc finger protein
• snail family zinc finger 1
• snail homolog 1
• Snail
• SNAIL1
• zinc finger protein SNAI1

Background

Snail, also called SNAIL1 or SNAI1, is a zinc-finger transcription factor belonging to the Snail superfamily and encoded by the SNAI1 gene (1,2). Snail was first discovered in Drosophila and has homologs in many species including vertebrates and humans (1,2). The Snail family members includes Snail (Snail1), Slug (Snail2), and Smuc (Snail3) (1,2). In humans, Snail is expressed in a number of tissues including placenta, brain, and skeletal muscle, but is most highly expressed by the kidneys (1). Snail functions in repression of E-cadherin transcription which is associated with epithelial-mesenchymal transition (EMT) that is especially prominent during embryonic development (1-5). Along with Snail, other related EMT-inducing transcription factors (EMT-TFs) include the Twist and ZEB protein families (3). Snail is synthesized as a protein of 264 amino acids (aa) with an N-terminal SNAG domain, a serine-rich domain (SRD), nuclear export sequences (NES), and four C-terminal zinc-finger binding domains, with a theoretical molecular weight of 29 kDa (1,3). Snail activity is largely regulated through post-translational modifications such as phosphorylation, ubiquitination, and glycosylation, which impacts Snail's localization and stability, amongst other things (1-3, 5).

In addition to its role in embryonic development, Snail-induced EMT is also associated with cancer metastasis (1-5). Snail is expressed in a variety of cancer lines including breast cancer, cervical carcinoma, and colorectal carcinoma, and typically results in increased migration, invasion, and metastasis (1). Accordingly, Snail expression is also correlated with drug resistance and tumor recurrence (1-5). Chemical inhibitors that target Snail have shown some promise in reducing or eliminating Snail-induced EMT, increasing E-cadherin expression, and increasing tumor regression (1).

1. Kaufhold, S., & Bonavida, B. (2014). Central role of Snail1 in the regulation of EMT and resistance in cancer: a target for therapeutic intervention. Journal of Experimental & Clinical Cancer Research. https://doi.org/10.1186/s13046-014-0062-0

2. Wang, Y., Shi, J., Chai, K., Ying, X., & Zhou, B. P. (2013). The Role of Snail in EMT and Tumorigenesis. Current Cancer Drug Targets. https://doi.org/10.2174/15680096113136660102

3. Kang, E., Seo, J., Yoon, H., & Cho, S. (2021). The Post-Translational Regulation of Epithelial-Mesenchymal Transition-Inducing Transcription Factors in Cancer Metastasis. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms22073591

4. Seo, J., Ha, J., Kang, E., & Cho, S. (2021). The role of epithelial-mesenchymal transition-regulating transcription factors in anti-cancer drug resistance. Archives of Pharmacal Research. https://doi.org/10.1007/s12272-021-01321-x

5. Baulida, J., Diaz, V. M., & Herreros, A. G. (2019). Snail1: A Transcriptional Factor Controlled at Multiple Levels. Journal of Clinical Medicine. https://doi.org/10.3390/jcm8060757

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Snail Antibody (NBP2-27293)(15)

Publications using NBP2-27293

• Abrahamsson A, Boroojeni FR, Naeimipour S et Al. Increased matrix stiffness enhances pro-tumorigenic traits in a physiologically relevant breast tissue- monocyte 3D model Acta Biomater 2024-02-20 [PMID: 38382828]
• Zhu X, Lu J, Rao J et al. Cross Talk between ST2 and Transforming Growth Factor-? Receptor Signaling Promotes Renal Fibrosis The American journal of pathology 2023-05-24 [PMID: 37236504]
• Huang D, Duan H, Huang H et al. Cisplatin resistance in gastric cancer cells is associated with HER2 upregulation-induced epithelial-mesenchymal transition. Sci Rep 2016-02-05 [PMID: 26846307] (WB, IHC-P, Human)
• Abrahamsson A, Rasti Boroojeni F, Naeimipour S et al. Matrix Stiffness Enhances Pro-Tumorigenic Traits of ER-Positive but not ER-Negative Breast Cancer in a&nbsp;Physiologically Relevant 3D Model SSRN 2023-08-17 (ICC/IF, Human)
• Linling Ju, Qian Zhou, Qianyi Qi, Yongjun She, Weihua Cai, Yali Cao, Rujian Lu, Jianguo Shao, Lin Chen circSLCO1B7 suppresses the malignant progression of hepatocellular carcinoma via the miR-556-3p/DAB2IP axis Aging (Albany NY) 2023-11-30 [PMID: 38015711]
• Piero Campolmi, Lavinia Quintarelli, Irene Fusco, Lara Ronconi, Tiziano Zingoni Clinical evidence of 595 nm pulse dye laser treatment for viral warts on hands and feet. Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) 2023-09-28 [PMID: 37753676]
• PL Hsu, CW Chien, YA Tang, BW Lin, SC Lin, YS Lin, SY Chen, HS Sun, SJ Tsai Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis Science Advances, 2023-04-12;9(15):eade3422. 2023-04-12 [PMID: 37043564] (Western Blot, Immunohistochemistry-Paraffin, Human)
• Bychkov, ML;Kirichenko, AV;Mikhaylova, IN;Paramonov, AS;Yastremsky, EV;Kirpichnikov, MP;Shulepko, MA;Lyukmanova, EN; Extracellular Vesicles Derived from Acidified Metastatic Melanoma Cells Stimulate Growth, Migration, and Stemness of Normal Keratinocytes Biomedicines [PMID: 35327461] (WB, Human)
• Jin JQ, Han JS, Ha J Et al. Lobeglitazone, A Peroxisome Proliferator-Activated Receptor-Gamma Agonist, Inhibits Papillary Thyroid Cancer Cell Migration and Invasion by Suppressing p38 MAPK Signaling Pathway Endocrinology and metabolism (Seoul, Korea) 2021-10-01 [PMID: 34645125] (WB, Human)
• Lu R, Zhou Q, Ju L Et Al. Upregulation of TRIP13 promotes the malignant progression of lung cancer via the EMT pathway Oncology reports 2021-08-01 [PMID: 34184074] (WB)
• Meng L, Yue X, Zhou D, Li H Long non coding RNA OIP5 AS1 promotes metastasis of breast cancer via miR 340 5p/ZEB2 axis Oncol Rep 2020-08-10 [PMID: 32945479] (WB, Human)
• Ponandai-Srinivasan S, Saare M, et al. Syndecan-1 modulates the invasive potential of endometrioma via TGF-beta signalling in a subgroup of women with endometriosis. Hum Reprod 2020-10-01 [PMID: 32897364] (Simple Western)
• He C, Plattner R, Rangnekar V et al. Potential protein markers for breast cancer recurrence: a retrospective cohort study. Cancer Causes Control. 2018-11-28 [PMID: 30488343] (IF/IHC, Human)
• Wu J, Zhang Y, Cheng R et al. Expression of EMT regulators TWIST, SLUG, and SNAIL in follicular thyroid tumours may relate to widely invasive, poorly differentiated, and distant metastasis. Histopathology. 2018-10-28 [PMID: 30368884] (IF/IHC, Human)
• Wang Y, Sun J, Wei X et al. Decrease of miR-622 expression suppresses migration and invasion by targeting regulation of DYRK2 in colorectal cancer cells. Onco Targets Ther. 2017-03-06 [PMID: 28260923] (WB, Human)

FAQs for Snail Antibody (NBP2-27293). (Showing 1 - 2 of 2 FAQs).

1. There are many kinds of antibodies you supply against SNAIL. Could you give me your recommendation? Which is the best for the IHC-P experiments the species of my samples to be tested is human.
   • Our SNAIL antibody with catalog # NBP1-19529 has been successfully validated for IHC-P in paraffin-embedded human lung carcinoma tissue and I would highly recommend you to use the same for your samples too. The working dilutions of this antibody for IHC-P ranges from 1:50 - 1:200 and beside IHC, you can use this antibody for Western Blot and Immunofluorescence also.
2. Could you please let me know the secondary antibody recommended for this particular primary antibody (Catalog NumberNBP2-27293) so that I could order both primary & secondary antibodies.
   • Our SNAIL antibody with catalogue number NBP2-27293 is a rabbit polyclonal, and as such you would require an anti-rabbit secondary to detect it. You can see our full range of anti-rabbit secondary antibodies at the following link, and by using the search filters at the left hand side of the webpage you can select a suitably conjugated product for your research: View Secondary Antibodies

Bioinformatics

• Gene Symbol: SNAI1
• Uniprot:
  • O95863()