SMC2 Antibody - BSA Free

Western Blot: SMC2 Antibody [NBP2-49655] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251 MG

Immunocytochemistry/Immunofluorescence: SMC2 Antibody [NBP2-49655] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.

Immunohistochemistry-Paraffin: SMC2 Antibody [NBP2-49655] - Staining of human tonsil shows nuclear positivity in germinal center and non germinal center.

• Reactivity: Hu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SMC2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SMC2 Antibody - BSA Free (NBP2-49655) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: LSRDIGRLKETYEALLARFPNLRFAYKDPEKNWNRNCVKGLVASLISVKDTSATTALELVAGERLYNVVVDTEVTGKKLLERGELKRRYTIIPLNKISARCIAPETLRVAQNLVGPD
• Predicted Species: Rat (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  SMC2 Recombinant Protein Antigen (NBP2-49655PEP)

Reactivity Notes

Mouse (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SMC2 Antibody - BSA Free

• CAP-E
• CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast)
• Chromosome-associated protein E
• FLJ10093
• hCAP-EXCAP-E homolog
• SMC protein 2
• SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
• SMC2 structural maintenance of chromosomes 2-like 1
• SMC-2
• SMC2L1
• structural maintenance of chromosomes (SMC) family member
• structural maintenance of chromosomes 2
• structural maintenance of chromosomes protein 2

Background

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMC2