Western Blot: SMC1 Antibody [NB100-204] - Detection of human SMC1 in HeLa nuclear extracts (50 ug), Xenopus oocytes (100 ug), and MEF whole cell lysate (100 ug) in Western blot using NB 100-204.
Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - Sample: FFPE section of human breast carcinoma.
Biological Strategies: Western Blot: SMC1 Antibody [NB100-204] - Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3.
Immunoprecipitation: SMC1 Antibody [NB100-204] - Detection of Human SMC1 by Western Blot of Immunoprecipitates.
Simple Western: SMC1 Antibody [NB100-204] - Simple Western lane view shows a specific band for SMC1 in 0.5 mg/ml of Hek293 (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using ...read more
This antibody can be used for Western blot and immunofluorescence. In western blot a band is seen around 160 kDa representing SMC1. ELISA and Immunoprecipitation were reported in scientific literature. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-224, NB100-206
Alternate Names for SMC1 Antibody
DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
segregation of mitotic chromosomes 1
SMC protein 1A
SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
SMC1 structural maintenance of chromosomes 1-like 1
structural maintenance of chromosomes 1A
structural maintenance of chromosomes protein 1A
SMC1 is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of the cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, SMC1 works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Nakamura K, Fike F, Haghayegh S. A-TWinnipeg: Pathogenesis of rare ATM missense mutation c.6200C>A with decreased protein expression and downstream signaling, early-onset dystonia, cancer, and life-threatening radiotoxicity. Molecular Genetics & Genomic Medicine 2/2/2014 (WB, Human)
Nayler S, Gatei M, Kozlov S et al. Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells: Induced Pluripotent Stem Cells from Ataxia-Telangiectasia Recapitulate the Cellular Phenotype Stem Cells Trans Med 2012 June (WB, Human)
FAQs for SMC1 Antibody (NB100-204). (Showing 1 - 2 of 2 FAQs).
I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.
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