SMC1 Antibody

Images

 
Western Blot: SMC1 Antibody [NB100-204] - Detection of human SMC1 in HeLa nuclear extracts (50 ug), Xenopus oocytes (100 ug), and MEF whole cell lysate (100 ug) in Western blot using NB 100-204.
Immunohistochemistry-Paraffin: SMC1 Antibody [NB100-204] - Sample: FFPE section of human breast carcinoma.
Western Blot: SMC1 Antibody [NB100-204] - Whole cell lysate from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3.
Immunoprecipitation: SMC1 Antibody [NB100-204] - Detection of Human SMC1 by Western Blot of Immunoprecipitates.
Simple Western: SMC1 Antibody [NB100-204] - Simple Western lane view shows a specific band for SMC1 in 0.5 mg/ml of Hek293 (left) and HeLa (right) lysate. This experiment was performed under reducing conditions using ...read more

Product Details

Summary
Reactivity Hu, Mu, XpSpecies Glossary
Applications WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP, PLA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

SMC1 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1175 and the C-terminus of human Structural Maintenance of Chromosomes 1 using the numbering given in entry NP_006297.2
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000- 1:10000
  • Simple Western 1:100
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:250- 1:1000
  • Immunohistochemistry 1:500 - 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 1:10-1:500
  • Proximity Ligation Assay 1:1000-1:10000
Application Notes
This antibody can be used for Western blot and immunofluorescence. In western blot a band is seen around 160 kDa representing SMC1. ELISA and Immunoprecipitation were reported in scientific literature.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Positive Control
HeLa Lysate (NB800-PC9)
SMC1 Lysate (NBL1-16236)
Publications
Read Publications using
NB100-204 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Notes

This anitbody can be used as the primary antibody in a PLA assay with the following as complementing antibodies:NB100-322, NB100-224, NB100-206

Alternate Names for SMC1 Antibody

  • CDLS2
  • DXS423ESMC1 structural maintenance of chromosomes 1-like 1 (yeast)
  • KIAA0178DKFZp686L19178
  • SB1.8
  • SB1.8MGC138332
  • segregation of mitotic chromosomes 1
  • SMC protein 1A
  • SMC1 (structural maintenance of chromosomes 1, yeast)-like 1
  • SMC1 structural maintenance of chromosomes 1-like 1
  • SMC1
  • SMC1A
  • SMC-1A
  • SMC-1-alpha
  • SMC1L1
  • SMC1SMC1alpha
  • Smcb
  • structural maintenance of chromosomes 1A
  • structural maintenance of chromosomes protein 1A

Background

SMC1 is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of the cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, SMC1 works as a downstream effector in the ATM/NBS1 branch of S-phase checkpoint.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB100-204
Species: Hu, Mu, Xp
Applications: WB, Simple Western, ELISA, ICC/IF, IHC, IHC-P, IP, PLA

Publications for SMC1 Antibody (NB100-204)(33)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 6 applications: ELISA, ICC/IF, IHC-P, IP, WB, WB,IP.


Filter By Application
ELISA
(1)
ICC/IF
(1)
IHC-P
(1)
IP
(3)
WB
(13)
WB,IP
(4)
All Applications
Filter By Species
Human
(15)
Mouse
(5)
All Species
Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using NB100-204 Applications Species
Wang C, Jette N, Moussienko D et al. ATM-Deficient Colorectal Cancer Cells Are Sensitive to the PARP Inhibitor Olaparib. Transl Oncol. 2017 Feb 06 [PMID: 28182994] (WB, Human) WB Human
Batenburg NL, Thompson EL, Hendrickson EA, Zhu XD. Cockayne syndrome group B protein regulates DNA double-strand break repair and checkpoint activation. EMBO J. 2015 Mar 27 [PMID: 25820262] (WB, Mouse) WB Mouse
Houlihan SL, Feng Y. The scaffold protein Nde1 safeguards the brain genome during S phase of early neural progenitor differentiation. Elife. 2014 Sep 23 [PMID: 25245017] (IHC-P, Mouse) IHC-P Mouse
Puccini J, Shalini S, Voss AK et al. Loss of caspase-2 augments lymphomagenesis and enhances genomic instability in Atm-deficient mice. Proc Natl Acad Sci U S A 2013 Dec 3 [PMID: 24248351] (WB, Mouse) WB Mouse
Daniel JA, Pellegrini M, Lee BS et al. Loss of ATM kinase activity leads to embryonic lethality in mice J Cell Biol 2012 Aug 6 [PMID: 22869595] (WB, Mouse) WB Mouse
Gatei M, Jakob B, Chen P et al. ATM protein-dependent phosphorylation of Rad50 protein regulates DNA repair and cell cycle control. J Biol Chem. Sep. 2011 [PMID: 21757780] (WB, Human) WB Human
Devgan SS, Sanal O, Doil C et al. Homozygous deficiency of ubiquitin-ligase ring-finger protein RNF168 mimics the radiosensitivity syndrome of ataxia-telangiectasia. Cell Death Differ;18(9):1500-1506. 2011 Sep. [PMID: 21394101]
Rajesh C, Baker DK, Pierce AJ, Pittman DL. The splicing-factor related protein SFPQ/PSF interacts with RAD51D and is necessary for homology-directed repair and sister chromatid cohesion Nucleic Acids Res 2011 Jan [PMID: 20813759] (WB, Mouse) WB Mouse
Hu H, Du L, Nagabayashi G et al. ATM is down-regulated by N-Myc-regulated microRNA-421. Proc Natl Acad Sci U S A;107(4):1506-11. 2010 Jan 26. [PMID: 20080624] (WB, ELISA, Human) WB, ELISA Human
Yoshizawa-Sugata N, Masai H. Roles of human AND-1 in chromosome transactions in S phase. J Biol Chem 2009 Jul [PMID: 19439411]
Show All 33 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMC1 Antibody (NB100-204). (Showing 1 - 2 of 2 FAQs).

  1. I would like to use one of your anti-smc1 antibodies to detect smc1 in an invertebrate. Your different antibodies are raised against different peptides derived from different regions possibly well-conserved, but not completely, in the organism that interests me (Schistosoma mansoni). Would it be possible to know the sequence of the different peptides that was used to generate the different antibodies, or failing that, could you analyze the corresponding sequence in my species of interest in order to determine whether or not it corresponds to one of the different peptides?
    • My suggestion to you would be to look at the homology between the protein expressed in your species and the protein expressed in the reactive species of the antibodies. A homology of >90% is a great start, >80% is acceptable. Please view our list of smc1 antibodies we currently offer and there are 27 products.
  2. My sequence of interest have >80% homology with the N-term and the C-term parts of human smc1. Your antibodies NB100-78322 and NB100-204 recognized the N-term and C-term of human smc1 respectively, therefore it’s possible that the cross reactivity can happen. I would be very interested to test these antibodies (NB100-78322 and NB100-204). Can you provide me free sample (few ul) to do western blot. I will share the testing result with you.
    • We do not provide free samples for testing. If you are interested in testing these antibodies in an unlisted species or application, you would qualify for the Innovator's Rewards program. The way the program works is that you would purchase the antibody of interest, perform your testing using appropriate controls and then share your data with us. Upon review of your data we would issue you a voucher for a free antibody. You can find more details here.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC1A
Entrez