GRASP65 Antibody (OTI5G8)

Images

 
Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Staining in Hela cells: red CFTR, Green GRASP65 and Blue is DAPI. Image from a verified customer review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human prostate tissue using anti-GRASP65 mouse monoclonal antibody.
Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRASP65 antibody, and then ...read more
Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP65 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.
Immunocytochemistry: GRASP65 Antibody (5G8) [NBP2-02665] - Golgi localization in human Wi26 cells. Works for ICC on MeOH and PFA-fixed cells 1:100-1:200 dilution WB on human lung fibroblasts 1:2000 in TBST (membrane ...read more
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Detection in cultured pig trabecular meshwork cells. Image from a verified customer review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRASP65 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human Kidney tissue using anti-GRASP65 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human pancreas tissue using anti-GRASP65 mouse monoclonal antibody.
Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of Jurkat cells, using anti-GRASP65 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Po, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, ICC/IF
Clone
OTI5G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

GRASP65 Antibody (OTI5G8) Summary

Immunogen
Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GORASP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-2000
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Immunocytochemistry 1:100-1:200
Application Notes
ICC/IF reported in a customer review.
Theoretical MW
46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-02665 in the following applications:

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GRASP65 Antibody (OTI5G8)

  • Golgi peripheral membrane protein p65
  • Golgi phosphoprotein 5MGC118897
  • golgi reassembly stacking protein 1, 65kDa
  • Golgi reassembly-stacking protein 1
  • Golgi reassembly-stacking protein of 65 kDa
  • GRASP65MGC118894
  • P6565 kDa

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRASP65 Antibody (NBP2-02665) (0)

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Reviews for GRASP65 Antibody (NBP2-02665) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Pig.

Reviews using NBP2-02665:
Filter by Applications
ICC
(2)
IF
(1)
All Applications
Filter by Species
Human
(2)
Pig
(1)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry GRASP65 NBP2-02665
Enlarge
reviewed by:
Julian Nuechel
ICC Human 07/11/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast cell line
SpeciesHuman
LotA001

Comments

CommentsWorks for IF on MeOH and PFA-fixed cells 1:100-1:200 dilution
WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk)
Immunocytochemistry GRASP65 NBP2-02665
Enlarge
reviewed by:
Anonymous
ICC Pig 11/28/2016
View

Summary

ApplicationImmunocytochemistry
Sample Testedabecular meshwork cells
SpeciesPig
Immunofluorescence GRASP65 NBP2-02665
Enlarge
reviewed by:
Anonymous
IF Human 09/22/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
LotA01

Blocking

Blocking Details1X PBS/5% BSA 5% Triton™ X-100

Primary Anitbody

Dilution RatioGRASP65 Antibody (NBP2-02665) 1:20

Secondary Antibody

Secondary DescriptionGoat anti-mouse 488
Secondary Manufacturer Cat#Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate A-21202 Thermoscientific
Secondary Concentration1:400

Details

Detection NotesZeiss LSM 700 Confocal
Fixation DetailsCells grown on glass coverslips were washed and fixed using 4% Paraformaldehyde (in PBS),
Wash DescriptionPBS

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRASP65 Antibody (NBP2-02665) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72144AF350
Alexa Fluor 405 NBP2-72144AF405
Alexa Fluor 488 NBP2-72144AF488
Alexa Fluor 532 NBP2-72144AF532
Alexa Fluor 594 NBP2-72144AF594
Alexa Fluor 647 NBP2-72144AF647
Alexa Fluor 700 NBP2-72144AF700
Alexa Fluor 750 NBP2-72144AF750
Allophycocyanin NBP2-72144APC
Allophycocyanin/Cy7 NBP2-72144APCCY7
Biotin NBP2-72144B
DyLight 350 NBP2-72144UV
DyLight 405 NBP2-72144V
DyLight 488 NBP2-72144G
DyLight 550 NBP2-72144R
DyLight 594 NBP2-72144DL594
DyLight 650 NBP2-72144C
DyLight 680 NBP2-72144FR
DyLight 755 NBP2-72144IR
FITC NBP2-72144F
HRP NBP2-72144H
Janelia Fluor 549 NBP2-72144JF549
Janelia Fluor 646 NBP2-72144JF646
PE NBP2-72144PE
PE/Atto594 NBP2-72144PEATT594
PE/Cy5.5 NBP2-72144PECY55
PE/Cy7 NBP2-72144PECY7
PerCP NBP2-72144PCP

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Julian Nuechel
07/11/2017
Application: ICC
Species: Human

Anonymous
11/28/2016
Application: ICC
Species: Pig

Anonymous
09/22/2015
Application: IF
Species: Human

Bioinformatics

Gene Symbol GORASP1
Entrez