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GRASP65 Antibody (OTI5G8)

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Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.
Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GRASP65 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Staining in Hela cells: red CFTR, Green GRASP65 and Blue is DAPI. Image from a verified customer review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human prostate tissue using anti-GRASP65 mouse monoclonal antibody.
Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GRASP65 antibody, and then ...read more
Western Blot: GRASP65 Antibody (5G8) [NBP2-02665] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRASP65 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell ...read more
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRASP65.
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - GRASP65 Antibody (5G8) [NBP2-02665] - Golgi localization in human Wi26 cells. Works for ICC on MeOH and PFA-fixed cells 1:100-1:200 ...read more
Immunocytochemistry/ Immunofluorescence: GRASP65 Antibody (OTI5G8) [NBP2-02665] - Detection in cultured pig trabecular meshwork cells. Image from a verified customer review.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GRASP65 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human Kidney tissue using anti-GRASP65 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: GRASP65 Antibody (5G8) [NBP2-02665] - Staining of paraffin-embedded Human pancreas tissue using anti-GRASP65 mouse monoclonal antibody.
Flow Cytometry: GRASP65 Antibody (5G8) [NBP2-02665] - Analysis of Jurkat cells, using anti-GRASP65 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

Product Details

Summary
Reactivity Hu, Rt, Po, Ca, Pm, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
OTI5G8
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.6 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-72144AF350
Alexa Fluor 405
NBP2-72144AF405
Alexa Fluor 488
NBP2-72144AF488
Alexa Fluor 532
NBP2-72144AF532
Alexa Fluor 594
NBP2-72144AF594
Alexa Fluor 647
NBP2-72144AF647
Alexa Fluor 700
NBP2-72144AF700
Alexa Fluor 750
NBP2-72144AF750
Allophycocyanin
NBP2-72144APC
Allophycocyanin/Cy7
NBP2-72144APCCY7
Biotin
NBP2-72144B
DyLight 350
NBP2-72144UV
DyLight 405
NBP2-72144V
DyLight 488
NBP2-72144G
DyLight 550
NBP2-72144R
DyLight 594
NBP2-72144DL594
DyLight 650
NBP2-72144C
DyLight 680
NBP2-72144FR
DyLight 755
NBP2-72144IR
FITC
NBP2-72144F
HRP
NBP2-72144H
Janelia Fluor 549
NBP2-72144JF549
Janelia Fluor 646
NBP2-72144JF646
PE
NBP2-72144PE
PE/Atto594
NBP2-72144PEATT594
PE/Cy5.5
NBP2-72144PECY55
PE/Cy7
NBP2-72144PECY7
PerCP
NBP2-72144PCP
View Available Formulations
Catalog# & Formulation Size Price
Azide and BSA Free
NBP2-72144
100% Guarantee on every product

GRASP65 Antibody (OTI5G8) Summary

Immunogen
Full length human recombinant protein of human GORASP1 (NP_114105) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GORASP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:100
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000-2000
Application Notes
GRASP65 antibody validated for ICC/IF from a verified customer review.
Theoretical MW
46.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-02665 in the following applications:
Publications
Read Publication using NBP2-02665.

Reactivity Notes

Porcine reactivity reported from a verified customer review.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.6 mg/ml
Purity
Immunogen affinity purified

Alternate Names for GRASP65 Antibody (OTI5G8)

  • Golgi peripheral membrane protein p65
  • Golgi phosphoprotein 5MGC118897
  • golgi reassembly stacking protein 1, 65kDa
  • Golgi reassembly-stacking protein 1
  • Golgi reassembly-stacking protein of 65 kDa
  • GRASP65MGC118894
  • P6565 kDa

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a membrane protein involved in establishing the stacked structure of the Golgi apparatus. It is a caspase-3 substrate, and cleavage of this encoded protein contributes to Golgi fragmentation in apoptosis. This encoded protein can form a complex with the Golgi matrix protein GOLGA2, and this complex binds to the vesicle docking protein p115. Several alternatively spliced transcript variants of this gene have been identified, but their full-length natures have not been determined. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Flow Cytometry: ASCC1 Antibody (OTI6G5) [NBP2-01345] - ASCC1 Antibody (6G5) [NBP2-01345] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ASCC1 antibody, and then analyzed by Flow cytometry.
Western Blot: ASCC1 Antibody (OTI6G5) [NBP2-01345] - ASCC1 Antibody (6G5) [NBP2-01345] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ASCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ASCC1.
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RelA/NFkB p65 ELISA K ...
NBP2-29661
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Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Left: NBP2-50037 staining (green) in HeLa cells, which were treated with serum-starvation for 36 hrs, followed by 2 hrs 20% FBS stimulation (bottom), or PBS treatment (top). Green c-Fos staining only localizes in the nuclei of stimulated cells, but not in un-stimulated cells. Cells are counter-stained with chicken pAb against Vimentin (NB300-223, red). Blue shows DAPI staining of nucleus. Middle: Mouse brain section (45 uM; fixed by transcardial perfusion with 4% PFA) labeled with NBP2-50037 using a standard HRP-DAB staining technique. Cells expressing c-Fos show dark color in nucleus. Right: Mouse cortical section labeled with NBP2-50037 (red) and rabbit polyclonal anti-NeuN (NBP1-92716, green) using IF confocal. Neurons positive for c-Fos and RBFOX3/NeuN appear to be yellow. Inset shows an enlarged image of NBP2-50037 staining. Nuclei are labeled with Dapi (blue).
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Immunocytochemistry/Immunofluorescence: ISYNA1 Antibody [NBP2-47415] - Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & cytosol.
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Immunohistochemistry-Paraffin: ISYNA1 Antibody [NBP2-47415] - Staining of human testis shows high expression.
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Antibody
AF3235
Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. Specific bands were detected for Neurofascin at approximately 140 and186 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (yellow; Catalog # NL016) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and Schwann cells (perinodal regions). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
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AKT [p Ser473]
Antibody - Pan Specif
AF887
Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120‑HD) for 15 minutes and MCF‑7 human breast cancer cell line untreated or treated with 100 ng/mL Recombinant Human IGF-1 (Catalog # 291‑G1) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Akt (S473) Pan Specific at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line and A549 human lung carcinoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF‑I (Catalog # 291-G1) for 15 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Akt (S473) at approximately 60 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Akt (S473) Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF887). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Serum-starved MCF‑7 human breast cancer cells were unstimulated (light orange filled histogram) or activated with 100 ng/mL Recombinant human IGF-1 (Catalog # 291-G1, dark orange filled histogram) for 15 minutes, then stained with Rabbit Anti-Human/Mouse/Rat Phospho‑Akt (S473) Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF887) or control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
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ERK1 Antibody (1E5)
NBP2-22203
Flow Cytometry: ERK1 Antibody (1E5) [NBP2-22203] - Flow cytometric analysis of Hela cells using ERK1 mouse mAb (blue) and negative control (red).
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of paraffin-embedded bladder cancer tissues using ERK1 mouse mAb with DAB staining.
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
JNK1 Antibody
(228601) [Unconjugate
MAB17761
Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
JNK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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CXCL8/IL-8
Antibody (6217) [Unco ...
MAB208
Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human IL-8/CXCL8 (20 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208). The ND50 is typically0.08-0.4 µg/mL.
IL-8/CXCL8 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human IL-8/CXCL8 Monoclonal Antibody (Catalog # MAB208) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
(10 Reviews)
(97 Publications)
Species: Hu
Applications: CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta), ICC, ICFlow, Neut, Simple Western, WB

Publications for GRASP65 Antibody (NBP2-02665)(1)


Reviews for GRASP65 Antibody (NBP2-02665) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Pig.

Reviews using NBP2-02665:
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ICC
(2)
IF
(1)
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(2)
Pig
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry GRASP65 NBP2-02665
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reviewed by:
Julian Nuechel
 ICC Human 07/11/2017
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Summary

ApplicationImmunocytochemistry
Sample TestedHuman fibroblast cell line
SpeciesHuman
LotA001

Comments

CommentsWorks for IF on MeOH and PFA-fixed cells 1:100-1:200 dilution
WB on human lung fibroblasts 1:2000 in TBST (membrane blocking with 5% skimmed milk)
Immunocytochemistry GRASP65 NBP2-02665
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reviewed by:
Verified Customer
 ICC Pig 11/28/2016
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Summary

ApplicationImmunocytochemistry
Sample Testedabecular meshwork cells
SpeciesPig
Immunofluorescence GRASP65 NBP2-02665
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reviewed by:
Verified Customer
 IF Human 09/22/2015
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Summary

ApplicationImmunofluorescence
Sample TestedHeLa cells
SpeciesHuman
LotA01

Blocking

Blocking Details1X PBS/5% BSA 5% Triton™ X-100

Primary Anitbody

Dilution RatioGRASP65 Antibody (NBP2-02665) 1:20

Secondary Antibody

Secondary DescriptionGoat anti-mouse 488
Secondary Manufacturer Cat#Donkey anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate A-21202 Thermoscientific
Secondary Concentration1:400

Details

Detection NotesZeiss LSM 700 Confocal
Fixation DetailsCells grown on glass coverslips were washed and fixed using 4% Paraformaldehyde (in PBS),
Wash DescriptionPBS

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GRASP65 Antibody (NBP2-02665) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-72144AF350
Alexa Fluor 405 NBP2-72144AF405
Alexa Fluor 488 NBP2-72144AF488
Alexa Fluor 532 NBP2-72144AF532
Alexa Fluor 594 NBP2-72144AF594
Alexa Fluor 647 NBP2-72144AF647
Alexa Fluor 700 NBP2-72144AF700
Alexa Fluor 750 NBP2-72144AF750
Allophycocyanin NBP2-72144APC
Allophycocyanin/Cy7 NBP2-72144APCCY7
Biotin NBP2-72144B
DyLight 350 NBP2-72144UV
DyLight 405 NBP2-72144V
DyLight 488 NBP2-72144G
DyLight 550 NBP2-72144R
DyLight 594 NBP2-72144DL594
DyLight 650 NBP2-72144C
DyLight 680 NBP2-72144FR
DyLight 755 NBP2-72144IR
FITC NBP2-72144F
HRP NBP2-72144H
Janelia Fluor 549 NBP2-72144JF549
Janelia Fluor 646 NBP2-72144JF646
PE NBP2-72144PE
PE/Atto594 NBP2-72144PEATT594
PE/Cy5.5 NBP2-72144PECY55
PE/Cy7 NBP2-72144PECY7
PerCP NBP2-72144PCP
Learn about Custom Antibody Labeling

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0
Immunocytochemistry GRASP65 NBP2-02665
Enlarge
 Julian Nuechel
07/11/2017
Application: ICC
Species: Human
read this review
Immunocytochemistry GRASP65 NBP2-02665
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 Verified Customer
11/28/2016
Application: ICC
Species: Pig
read this review
Immunofluorescence GRASP65 NBP2-02665
Enlarge
 Verified Customer
09/22/2015
Application: IF
Species: Human
read this review

Bioinformatics

Gene Symbol GORASP1
Entrez