SMC3 Antibody

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Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 ...read more
Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB
SMC3 Antibody [NB100-207] - Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. ...read more

Product Details

Summary
Reactivity Hu, Mu, Ce, Bv, PmSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

SMC3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1175 and the C-terminus (residue 1217) of human Structural Maintenance of Chromosomes 3 using the numbering given in entry NP_005436.1 (GeneID 9126).
Predicted Species
Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:10000 - 1:25000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 ul/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publications using
NB100-207 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Orangutan (100%). C. elegans reactivity reported in scientific literature (PMID: 30563860).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMC3 Antibody

  • Bamacan
  • BAMSMC protein 3
  • Basement membrane-associated chondroitin proteoglycan
  • BMH
  • chondroitin sulfate proteoglycan 6 (bamacan)
  • Chondroitin sulfate proteoglycan 6
  • Chromosome-associated polypeptide
  • CSPG6structural maintenance of chromosomes protein 3
  • hCAP
  • HCAPbamacan
  • SMC-3
  • SMC3L1CDLS3
  • structural maintenance of chromosomes 3

Background

Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMC3 Antibody (NB100-207)(8)

We have publications tested in 1 confirmed species: C. elegans.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
C. elegans
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB100-207 Applications Species
Bowman R, Balukof N, Ford T, Smolikove S. A Novel Role for a-importins and Akirin in Establishment of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans Genetics Dec 18 2018 [PMID: 30563860] (IHC, C. elegans) IHC C. elegans
Deardorff MA, Bando M, Nakato R et al. HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle. Nature 2012 Sep [PMID: 22885700]
Shiomi Y, Hayashi A, Ishii T et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol 2012 Jun [PMID: 22493068]
Leman AR, Noguchi C, Lee CY et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 2010 Mar [PMID: 20124417]
Terret ME, Sherwood R, Rahman S et al. Cohesin acetylation speeds the replication fork. Nature 2009 Nov [PMID: 19907496]
Adelfalk C, Janschek J, Revenkova E et al. Cohesin SMC1beta protects telomeres in meiocytes. J Cell Biol 2009 Oct [PMID: 19841137]
Rankin S, Ayad NG, Kirschner MW et al. Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates. Mol Cell 2005 Apr [PMID: 15837422]
Revenkova E, Eijpe M, Heyting C et al. Cohesin SMC1 beta is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination. Nat Cell Biol 2004 Jun [PMID: 15146193]

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Bioinformatics

Gene Symbol SMC3
Entrez
Uniprot