SMC3 Antibody


Western Blot: SMC3 Antibody [NB100-207] - Whole cell lysate (10 ug) from HEK293T, HeLa, Jurkat, LNCaP, U2OS, A-549, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinitypurified rabbit anti-SMC3 more
Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: SMC3 Antibody [NB100-207] - Section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SMC3 used at 1:5,000 (0.2ug/ml). Detection: DAB
SMC3 Antibody [NB100-207] - Detection of human SMC3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. more

Product Details

Reactivity Hu, Mu, Ce, Bv, PmSpecies Glossary
Applications WB, IHC, IP
1.0 mg/ml

SMC3 Antibody Summary

The immunogen recognized by this antibody maps to a region between residue 1175 and the C-terminus (residue 1217) of human Structural Maintenance of Chromosomes 3 using the numbering given in entry NP_005436.1 (GeneID 9126).
Predicted Species
Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-10 ul/mg
  • Western Blot 1:10000 - 1:25000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Read Publications using
NB100-207 in the following applications:

Reactivity Notes

Orangutan (100%). C. elegans reactivity reported in scientific literature (PMID: 30563860).

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for SMC3 Antibody

  • Bamacan
  • BAMSMC protein 3
  • Basement membrane-associated chondroitin proteoglycan
  • BMH
  • chondroitin sulfate proteoglycan 6 (bamacan)
  • Chondroitin sulfate proteoglycan 6
  • Chromosome-associated polypeptide
  • CSPG6structural maintenance of chromosomes protein 3
  • hCAP
  • HCAPbamacan
  • SMC-3
  • structural maintenance of chromosomes 3


Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis and in chromosome movement.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMC3 Antibody (NB100-207)(9)

We have publications tested in 2 confirmed species: Human, C. elegans.

We have publications tested in 2 applications: Chemotaxis, IF/IHC.

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C. elegans
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Showing Publications 1 - 9 of 9.
Publications using NB100-207 Applications Species
Tao, H, Lambert, J P Et al. IRX3/5 regulate mitotic chromatid segregation and limb bud shape. Development 2020-10-05 [PMID: 32907847] (Chemotaxis, Human) Chemotaxis Human
Bowman R, Balukof N, Ford T, Smolikove S. A Novel Role for a-importins and Akirin in Establishment of Meiotic Sister Chromatid Cohesion in Caenorhabditis elegans Genetics 2018-12-18 [PMID: 30563860] (IF/IHC, C. elegans) IF/IHC C. elegans
Deardorff MA, Bando M, Nakato R et al. HDAC8 mutations in Cornelia de Lange syndrome affect the cohesin acetylation cycle. Nature 2012-09-01 [PMID: 22885700]
Shiomi Y, Hayashi A, Ishii T et al. Two different replication factor C proteins, Ctf18 and RFC1, separately control PCNA-CRL4Cdt2-mediated Cdt1 proteolysis during S phase and following UV irradiation. Mol Cell Biol. 2012-06-01 [PMID: 22493068]
Leman AR, Noguchi C, Lee CY et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 2010-03-01 [PMID: 20124417]
Terret ME, Sherwood R, Rahman S et al. Cohesin acetylation speeds the replication fork. Nature 2009-11-01 [PMID: 19907496]
Adelfalk C, Janschek J, Revenkova E et al. Cohesin SMC1beta protects telomeres in meiocytes. J Cell Biol 2009-10-01 [PMID: 19841137]
Rankin S, Ayad NG, Kirschner MW et al. Sororin, a substrate of the anaphase-promoting complex, is required for sister chromatid cohesion in vertebrates. Mol Cell 2005-04-01 [PMID: 15837422]
Revenkova E, Eijpe M, Heyting C et al. Cohesin SMC1 beta is required for meiotic chromosome dynamics, sister chromatid cohesion and DNA recombination. Nat Cell Biol 2004-06-01 [PMID: 15146193]

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Gene Symbol SMC3