Biological Strategies: Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.
Independent Antibodies: Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : ...read more
The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Immunogen affinity purified
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NB 100-373 may be used for Western Blot, where a band at ~155 kDa is seen, representing SMC2 protein. Suggested working dilutions: * Western Blot - 1:5,000-1:20,000 Immunofluorescence - 1:200-1:400 Immunoprecipitation - 2-10 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application.
CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast)
Chromosome-associated protein E
SMC protein 2
SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
SMC2 structural maintenance of chromosomes 2-like 1
structural maintenance of chromosomes (SMC) family member
structural maintenance of chromosomes 2
structural maintenance of chromosomes protein 2
Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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