SMC2 Antibody

Images

 
Biological Strategies: Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Western Blot: SMC2 Antibody [NB100-373] - Detection of Human SMC2 on HeLa whole cell lysate using NB100-373.
Independent Antibodies: Western Blot: SMC2 Antibody [NB100-373] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibod ies : ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IP, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SMC2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1150 and the C-terminus (residue 1197) of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMC2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:200-1:400
  • Immunoprecipitation 2-10 ug/mg lysate
  • Immunocytochemistry
Application Notes
NB 100-373 may be used for Western Blot, where a band at ~155 kDa is seen, representing SMC2 protein. Suggested working dilutions: * Western Blot - 1:5,000-1:20,000 Immunofluorescence - 1:200-1:400 Immunoprecipitation - 2-10 ug/mg lysate *The investigator should determine the optimal working dilution for a specific application.
Publications
Read Publications using NB100-373.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SMC2 Antibody

  • CAP-E
  • CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast)
  • Chromosome-associated protein E
  • FLJ10093
  • hCAP-EXCAP-E homolog
  • SMC protein 2
  • SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
  • SMC2 structural maintenance of chromosomes 2-like 1
  • SMC-2
  • SMC2L1
  • structural maintenance of chromosomes (SMC) family member
  • structural maintenance of chromosomes 2
  • structural maintenance of chromosomes protein 2

Background

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SMC2 Antibody (NB100-373)(6)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SMC2 Antibody (NB100-373) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SMC2
Entrez
Uniprot