SMC2 Antibody - BSA Free

Western Blot: SMC2 Antibody [NB100-372] - Samples : Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody : Affinity purified rabbit anti-SMC2 used at 0.1 ug/ml. Detection : Chemiluminescence with a 10 minute exposure.

Western Blot: SMC2 Antibody [NB100-372] - Whole cell lysate (90 ug) from mock transfected (E) or SMC2 transfected (T) HEK293T cells. Antibody used at 0.1 ug/ml.

Western Blot: SMC2 Antibody [NB100-372] - Samples: Whole cell lysate (50 ug) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMC2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SMC2 Antibody - BSA Free Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 950 and 1000 of human Structural Maintenance of Chromosomes 2 using the numbering given in entry NP_006435.1 (GeneID 10592).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SMC2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1
• Application Notes: This antibody may be used for Western Blot, where a band at ~155 kDa is seen, representing SMC2 protein. The investigator should determine the optimal working dilution for a specific application.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SMC2 Antibody - BSA Free

• CAP-E
• CAPESMC2 structural maintenance of chromosomes 2-like 1 (yeast)
• Chromosome-associated protein E
• FLJ10093
• hCAP-EXCAP-E homolog
• SMC protein 2
• SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
• SMC2 structural maintenance of chromosomes 2-like 1
• SMC-2
• SMC2L1
• structural maintenance of chromosomes (SMC) family member
• structural maintenance of chromosomes 2
• structural maintenance of chromosomes protein 2

Background

Structural Maintenance of Chromosome (SMC) proteins are required for nuclear processes, including chromosome condensation, chromatid cohesion, and DNA repair. Eukaryotic proteins are capable of binding DNA and possess a DNA-stimulated ATPase activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMC2
• Entrez:
  • 10592(Human)
• Uniprot:
  • NP_006435.1(Human)