Smad3 [p Ser423, p Ser425] Antibody [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ch, Xp, ZeSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Alexa Fluor 488

Order Details

Smad3 [p Ser423, p Ser425] Antibody [Alexa Fluor® 488] Summary

Immunogen
Anti-SMAD3 pS423pS425 antibody was prepared from whole rabbit serum produced by repeated immunizations with a dual phosphorylated synthetic peptide corresponding to a c-terminal region with Serine 423 and Serine 425 of human Smad3 protein.
Modification
p Ser423, p Ser425
Specificity
This antibody is directed against the phosphorylated form of human Smad3 protein at the pS423 and pS425 residues.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SMAD3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Xenopus laevis, Xenopus tropicalis.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Smad3 [p Ser423, p Ser425] Antibody [Alexa Fluor® 488]

  • DKFZp586N0721
  • DKFZp686J10186
  • hMAD-3
  • hSMAD3
  • HsT17436
  • JV15-2MGC60396
  • MAD homolog 3
  • mad protein homolog
  • MAD, mothers against decapentaplegic homolog 3 (Drosophila)
  • MAD, mothers against decapentaplegic homolog 3
  • mad3
  • MADH3mad homolog JV15-2
  • mothers against decapentaplegic homolog 3
  • Mothers against DPP homolog 3
  • SMA- and MAD-related protein 3
  • SMAD 3
  • SMAD family member 3HSPC193
  • SMAD, mothers against DPP homolog 3 (Drosophila)
  • SMAD, mothers against DPP homolog 3
  • Smad3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Smad3 Antibody (NBP1-77836AF488). (Showing 1 - 1 of 1 FAQ).

  1. I was wondering if you can recommend any primary polyclonal Smad3 antibody (reactivity: human) for gel supershifts?
    • We do provide a wide range of Smad3 antibodies, but unfortunately none of them have been tested for gel shift assay, so we can't guarantee they will work for an application never tested before.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-77836AF350
Alexa Fluor 405 NBP1-77836AF405
Alexa Fluor 488 NBP1-77836AF488
Alexa Fluor 532 NBP1-77836AF532
Alexa Fluor 594 NBP1-77836AF594
Alexa Fluor 647 NBP1-77836AF647
Alexa Fluor 700 NBP1-77836AF700
Alexa Fluor 750 NBP1-77836AF750
Allophycocyanin NBP1-77836APC
Allophycocyanin/Cy7 NBP1-77836APCCY7
Biotin NBP1-77836B
DyLight 350 NBP1-77836UV
DyLight 405 NBP1-77836V
DyLight 488 NBP1-77836G
DyLight 550 NBP1-77836R
DyLight 594 NBP1-77836DL594
DyLight 650 NBP1-77836C
DyLight 680 NBP1-77836FR
DyLight 755 NBP1-77836IR
FITC NBP1-77836F
HRP NBP1-77836H
Janelia Fluor 549 NBP1-77836JF549
Janelia Fluor 646 NBP1-77836JF646
PE NBP1-77836PE
PE/Atto594 NBP1-77836PEATT594
PE/Cy5.5 NBP1-77836PECY55
PE/Cy7 NBP1-77836PECY7
PerCP NBP1-77836PCP

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Bioinformatics

Gene Symbol SMAD3