Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

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Western Blot: Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) [NBP3-15092] - Western blot detection of Smad1 [p Ser463, p Ser465] in A549, U2OS, C6 cell lysates using Smad1 [p Ser463, p Ser465] Rabbit mAb (1:1000 ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clone
S07-4A3
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Smad1 [p Ser463, p Ser465] Antibody (S07-4A3) Summary

Additional Information
Recombinant Monoclonal Antibody
Immunogen
A synthetic phosphopeptide corresponding to residues surrounding Ser463/Ser465 of human Smad1 (Uniprot#: Q15797)
Modification
p Ser463, p Ser465
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
SMAD1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
50nM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
Preservative
0.01% Sodium Azide
Purity
Affinity purified

Alternate Names for Smad1 [p Ser463, p Ser465] Antibody (S07-4A3)

  • BSP1
  • BSP-1
  • hSMAD1
  • JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
  • MAD homolog 1
  • MADH1JV41
  • MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
  • Mad-related protein 1
  • mothers against decapentaplegic homolog 1
  • Mothers against DPP homolog 1
  • SMAD family member 1SMAD 1
  • SMAD, mothers against DPP homolog 1
  • Smad1
  • TGF-beta signaling protein 1
  • transforming growth factor-beta signaling protein 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SMAD1