SMAD1/5/9 Antibody (RM487)

Western Blot: SMAD1/5/9 Antibody (RM487)[NBP3-18834]Western Blot of mouse liver tissue lysate, using Anti-Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) rabbit monoclonal antibody (clone RM487) at a 1:1000 dilution. The phospho-specificity of RM487 was verified by peptide blocking using a control nonphospho-peptide or phosphor-peptide targeting residue Ser463/465 of Smad1 and Smad5 and residue Ser465/467 of Smad9.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: RM487
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

SMAD1/5/9 Antibody (RM487) Summary

• Description: Novus Biologicals Rabbit SMAD1/5/9 Antibody (RM487) (NBP3-18834) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A phospho-peptide corresponding to human Phospho-SMAD1 [Ser463/465]/ SMAD5 [Ser463/465]/ SMAD9 [Ser465/467]
• Specificity: This antibody reacts to SMAD1 and SMAD5 when phosphorylated at Ser463/465 and SMAD9 when phosphorylated at Ser465/467. There is no cross-reactivity to SMAD1, SMAD5, or SMAD9 that are not phosphorylated.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SMAD1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for SMAD1/5/9 Antibody (RM487)

• BSP1
• BSP-1
• hSMAD1
• JV4-1SMAD, mothers against DPP homolog 1 (Drosophila)
• MAD homolog 1
• MADH1JV41
• MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila)
• Mad-related protein 1
• mothers against decapentaplegic homolog 1
• Mothers against DPP homolog 1
• SMAD family member 1SMAD 1
• SMAD, mothers against DPP homolog 1
• Smad1
• TGF-beta signaling protein 1
• transforming growth factor-beta signaling protein 1

Background

Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFbeta/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MadH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFbeta and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFbeta signaling by interfering with TGFbeta-mediated phosphorylation of other Smad family members.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SMAD1