Sirtuin 3/SIRT3 Antibody - BSA Free

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Wild-type (WT) and SIRT3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Analysis of methanol-fixed A549, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Cal27 xenograft, using SIRT3 antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Western Blot: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Whole cell extracts (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SIRT3 antibody at a dilution of 1:1000.

Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP1-31029] - Human endometrial carcinoma. SIRT3 antibody [C3], C-term diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, KO, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Sirtuin 3/SIRT3 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Sirtuin 3/SIRT3 Antibody - BSA Free (NBP1-31029) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-Sirtuin 3/SIRT3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Sirtuin 3/SIRT3. The exact sequence is proprietary.
• Localization: Mitochondrion matrix
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SIRT3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:500-1:3000
• Application Notes: Use in Knockdown Validated reported in scientific literature (PMID:31931835).
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:31931835).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Sirtuin 3/SIRT3 Antibody - BSA Free

• EC 3.5.1
• EC 3.5.1.-
• hSIRT3
• mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
• NAD-dependent deacetylase sirtuin-3, mitochondrial
• SIR2L3
• SIR2L3silent mating type information regulation 2, S.cerevisiae, homolog 3
• sir2-like 3
• SIR2-like protein 3
• SIRT3
• sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
• sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3
• Sirtuin 3
• sirtuin type 3

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Sirtuin 3/SIRT3 Antibody (NBP1-31029)(1)

Publication using NBP1-31029

• Alpha-synuclein-induced mitochondrial dysfunction is mediated via a sirtuin 3-dependent pathway. Park J, Delenclos M, Faroqi A et al. Mol Neurodegener [PMID: 31931835] (WB, KD, IF/IHC, Human, Rat)

Bioinformatics

• Gene Symbol: SIRT3