| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-02984-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14289 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52562-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-51641SS | |||
| Azide and BSA Free | NBP2-80949 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4358-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4358-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27205SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14290 | |||
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts from wild type(WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Knockout-Validated-NBP3-02984-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts from wild type(WT) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of various cell lines, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP3-02984-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: Sirtuin 3/SIRT3 Antibody - Azide and BSA Free [Sirtuin 3/SIRT3] - Immunofluorescence analysis of NIH/3T3 cells using Sirtuin 3/SIRT3 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-02984_rabbit-polyclonal-sirtuin-3-sirt3-antibody-5220251640215.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Mouse kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0003.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Analysis of extracts of Mouse liver, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Western-Blot-NBP3-02984-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Human thyroid cancer using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0001.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 3/SIRT3 Antibody [NBP3-02984] - Rat kidney using SIRT3 antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-Immunohistochemistry-Paraffin-NBP3-02984-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-PCRP-SIRT3-1C10-Immunocytochemistry-Immunofluorescence-NBP3-13878-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Flow Cytometry: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Flow cytometric analysis of PFA-fixed HeLa cells. Sirtuin 3/SIRT3 antibody (PCRP-SIRT3-1C10) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-PCRP-SIRT3-1C10-Flow-Cytometry-NBP3-13878-img0001.jpg)
![Protein Array: Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10) [NBP3-13878] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 3/SIRT3 Antibody (PCRP-SIRT3-1C10).](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-PCRP-SIRT3-1C10-Protein-Array-NBP3-13878-img0004.jpg)
![Western Blot: Sirtuin 3/SIRT3 Antibody (6C12D1) [NBP2-52562] - Analysis using SIRT3 mAb against human SIRT3 (AA: 155-290) recombinant protein. (Expected MW is 41.3 kDa)](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6C12D1-Western-Blot-NBP2-52562-img0001.jpg "Western Blot: Sirtuin 3/SIRT3 Antibody (6C12D1) [NBP2-52562] - Analysis using SIRT3 mAb against human SIRT3 (AA: 155-290) recombinant protein. (Expected MW is 41.3 kDa)")
![Western Blot: Sirtuin 3/SIRT3 Antibody (6C12D1) [NBP2-52562] - Analysis using SIRT3 mAb against HEK293 (1) and SIRT3 (AA: 155-290)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6C12D1-Western-Blot-NBP2-52562-img0002.jpg)
![ELISA: Sirtuin 3/SIRT3 Antibody (6C12D1) [NBP2-52562] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize/Sirtuin-3-SIRT3-Antibody-6C12D1-ELISA-NBP2-52562-img0003.jpg)
![Western Blot: SIRT3 Overexpression Lysate (Adult Normal) [NBL1-15976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRT3.](http://images.novusbio.com/fullsize/SIRT3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15976-img0002.jpg "Western Blot: SIRT3 Overexpression Lysate (Adult Normal) [NBL1-15976] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SIRT3.")
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-NBP1-51641-img0015.jpg "Immunohistochemistry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain and blood and reduces the SIRT1 protein level in different brain regions. Confocal images from the hippocampus and basolateral amygdala of control and ESI mice reacted with SIRT1 antibody. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125) licensed under a CC-BY license.")
![Western Blot: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Western blot analysis using SIRT1 mouse mAb against MCF-7 (1), Jurkat (2), Hela (3), HEK293 (4) and A549 (5) cell lysates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Western-Blot-NBP1-51641-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - SIRT1 antibody was tested at 1:10 in Ntera2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0012.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using SIRT1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Analysis of NTERA-2 cells using SIRT1 mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunocytochemistry-Immunofluorescence-NBP1-51641-img0006.jpg)
![Immunohistochemistry-Paraffin: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Immunohistochemical analysis of paraffin-embedded lung cancer tissues (left) and kidney cancer tissues (right) using SIRT1 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Immunohistochemistry-Paraffin-NBP1-51641-img0003.jpg)
![Flow (Intracellular): Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - An intracellular stain was performed on RAW 246.7 cells with Sirtuin 1/SIRT1 Antibody (1F3) NBP1-51641APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Intracellular-NBP1-51641-img0014.jpg)
![Flow Cytometry: Sirtuin 1/SIRT1 Antibody (1F3) [NBP1-51641] - Flow cytometric analysis of K562 cells using SIRT1 mouse mAb (green) and negative control (purple).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-1F3-Flow-Cytometry-NBP1-51641-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Sirtuin 1/SIRT1 Antibody (1F3) - BSA Free [NBP1-51641] - Early social isolation (ESI) induces long-term changes in sirtuin1 (SIRT) mRNA expression in the mouse brain & blood & reduces the SIRT1 protein level in different brain regions. (a) The mRNA expression levels (relative expression levels=fold changes over control, normalized to PGK1 & TBP (Tata Binding Protein) genes) of SIRT1 & SIRT6 were significantly decreased in the brain of ESI compared with control mice. (b) In peripheral blood mononuclear cells (PBMCs) of ESI-treated mice, the expression level of SIRT1 mRNA was downregulated. ESI, n=9 (male (M)=6, female (F)=3); Control, n=7 (M=4, F=3) ***P<0.001; *P<0.05. (c) Confocal images showing SIRT1 (red) & NeuN (green) immunostaining plus 4,6-diamidino-2-phenylindole (DAPI) counterstaining (gray) in the brain of control mice. SIRT1 (red) colocalizes both with the neuronal marker NeuN (green) & with DAPI (gray). (d) Confocal images from the hippocampus & basolateral amygdala of control & ESI mice reacted with SIRT1 antibody. (e) Densitometric analysis of SIRT1 immunoreactivity in the motor cortex, striatum, hippocampus & basolateral amygdala revealed a significant reduction of SIRT1 immunoreactivity in ESI compared with control mice. The F/A ratio defines the mean fluorescence of individual samples (F) normalized to total surface (A). ESI, n=5; Control, n=5. ***P<0.001. Scale bars: (d) 100 μm; 25 μm. Image collected & cropped by CiteAb from the following publication (//www.nature.com/articles/tp2015125), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-51641_mouse-monoclonal-sirtuin-1-sirt1-antibody-1f3-310202416165619.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Knockdown-Validated-NBP1-49540-img0007.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout (KO) HeLa cell line. PVDF membrane was probed with 1:1000 of Rabbit Anti-Human SIRT1 Polyclonal Antibody (Catalog # NBP1-49540) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog #HAF008). Specific band was detected for SIRT1 at approximately 130 kDa (as indicated) in the parental HeLa cell line, but is not detectable in the knockout HeLa cell line. This experiment was conducted under reducing conditions.")
![<b>Genetic Strategies Validation. </b>Western Blot: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Analysis of SIRT1 in HeLa whole cell lyates.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP1-49540-img0004.jpg)
![Immunohistochemistry: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Immunohistochemical analysis of SIRT1 in human renal cell carcinoma.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Immunohistochemistry-NBP1-49540-img0005.jpg)
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP1-49540] - Simple Western lane view shows a specific band for SIRT1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP1-49540-img0006.jpg)
![Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Western-Blot-NBP2-27205-img0003.jpg "Western Blot: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Analysis of SIRT1 using SIRT1 antibody. HeLa lysate probed with 1) prebleed, 2) SIRT1 antibody and 3) mouse NIH 3T3 lysate probed with SIRT1 antibody at2 ug/ml. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Simple Western: Sirtuin 1/SIRT1 Antibody [NBP2-27205] - Simple Western lane view shows a specific band for Sirtuin 1/SIRT1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-Simple-Western-NBP2-27205-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-PCRP-SIRT1-1E11-Immunocytochemistry-Immunofluorescence-NBP3-13916-img0002.jpg "Immunocytochemistry/Immunofluorescence: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).")
![Protein Array: Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11) [NBP3-13916] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Sirtuin 1/SIRT1 Antibody (PCRP-SIRT1-1E11).](http://images.novusbio.com/fullsize/Sirtuin-1-SIRT1-Antibody-PCRP-SIRT1-1E11-Protein-Array-NBP3-13916-img0004.jpg)
