Western Blot: SIRT6 Antibody [NB100-2524] - Detection of SIRT6 in HeLa whole cell lysate.
Immunocytochemistry/ Immunofluorescence: SIRT6 Antibody [NB100-2524] - SIRT6 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 ...read more
This SIRT6 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis, where a band is seen at ~39 kDa. A higher MW band ~59 kDa may be seen with lower dilutions (ie: 2 ug/ml). In ICC/IF nuclear staining was observed. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
39 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 6
sirtuin type 6
SIRT6 (SIR2-like protein 6) is a member of the yeast silent information regulator 2 (SIR2) family and possesses both strong ADP-ribosyltransferase activity as well as protein deacetylase activity which is dependent on NAD(+). SIRT6 exerts deacetylase activity towards histone H3K9Ac and H3K56Ac, and alters histone H3 acetylation in telomeric chromatin during cell cycle's S-phase. It deacetylates histone H3K9Ac at NFkB target promoters and downregulates a subset of NFkB target genes. Upon DNA damage, SIRT6 promotes DNA end resection via deacetylation of RBBP8. SIRT6 also serves as a corepressor of the transcription factor HIF1 alpha for regulating the expression of multiple glycolytic genes to control glucose homeostasis, and is required for normal IGF1 serum levels. SIRT6 play critical roles in maintaining telomere integrity, fine-tuning aging-associated gene expression programs, preventing genomic instability, and maintaining metabolic homeostasis, thus impacting on several pathways relevant for cancer, metabolism, aging, cellular senescence and apoptosis.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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