SIRT4 Antibody

Images

 
Western Blot: SIRT4 Antibody [NB100-1406] - Staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: SIRT4 Antibody [NB100-1406] - Staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SIRT4 Antibody Summary

Immunogen
Peptide with sequence SRCGELLPLIDPC corresponding to C-Terminus according to NP_036372.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SIRT4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2-3 ug/ml
  • Peptide ELISA detection limit 1:28000
Application Notes
WB: Approx. 37 kDa band observed in human kidney and U937 lysates (calculated MW of 35.2 kDa band according to NP_036372.1). In transfected HEK293 transiently expressing SIRT4 a band of approx. 38 kDa is observed. This band is not observed in the non-transfected HEK293. IHC-P: Human pancreas shows staining of the islets of langerhans.
IHC: In paraffin embedded Skeletal Muscle shows spotty staining of striated fibres in longitudinal section.
Control
Kidney Lysate (NB820-59231)
SIRT4 Lysate (NBL1-15977)
Publications
Read Publications using
NB100-1406 in the following applications:

  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity:Rat, Mouse, Canine, Porcine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SIRT4 Antibody

  • EC 2.4.2.-
  • EC 3.5.1
  • MGC130046
  • MGC130047
  • MGC57437
  • SIR2L4NAD-dependent ADP-ribosyltransferase sirtuin-4
  • sir2-like 4
  • SIR2-like protein 4
  • sirtuin (silent mating type information regulation 2 homolog) 4 (S. cerevisiae)
  • sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 4
  • sirtuin 4
  • sirtuin type 4

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SIRT4 Antibody (NB100-1406)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol SIRT4
Entrez
Uniprot