Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063) - BSA Free

Western Blot: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Western blot analysis using NBP3-21546 in (1) HEK293 cell lysate; (2) HEK293 cell lysate treated with LP.

Immunohistochemistry: Sirtuin 1/SIRT1 [p Ser 47] Antibody (SR1063) [NBP3-21546] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21546.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: SR1063
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063) - BSA Free Summary

• Description: Novus Biologicals Rabbit Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063) - BSA Free (NBP3-21546) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-Sirtuin 1/SIRT1 [Ser47] (Uniprot #: Q96EB6)
• Modification: p Ser47
• Specificity: Detects endogenous levels of phospho-Sirtuin 1/SIRT1 [Ser47]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SIRT1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:2000
• Theoretical MW: 110 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Sirtuin 1/SIRT1 [p Ser47] Antibody (SR1063) - BSA Free

• EC 3.5.1
• S. cerevisiae, homolog) 1
• SIR2alpha
• SIR2L1
• SIR2-like protein 1
• SIRT1
• Sirtuin 1

Background

The S. cerevisiae silent information regulator 2 (Sir2) protein is an NAD+-dependent histone deacetylase (HDACs) that plays a critical role in transcriptional silencing, genome stability, and longevity (1-2). Part of the Sirtuin family (Sir2, Sir3, Sir3), yeast Sir2 is the founding member of Class III HDACs. A human homologue of Sir2 , SIRT1, regulates the activity of the p53 tumor suppressor and inhibits apoptosis (3-4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SIRT1