SH3BP2 [p Ser427] Antibody - BSA Free

Western Blot: SH3BP2 [p Ser427] Antibody [NB600-492] - Detection of endogenous protein present in unstimulated human whole cell lysates). The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of 60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.

Western blot analysis is shown using Affinity Purified anti-SH3BP2 pS427 antibody to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa corresponding to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

SH3BP2 [p Ser427] Antibody - BSA Free Summary

• Description: This product is an affinity purified antibody produced by immunoaffinity chromatography and is phospho specific for human pS427.
  
  Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: This affinity purified SH3BP2 [p Ser427] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 415-440 of Human SH3BP2 protein (SH3 Domain Binding Protein 2). (Uniprot: P78314)
• Modification: p Ser427
• Specificity: This product is an affinity purified antibody produced by immunoaffinity chromatography and is phospho specific for human SH3BP2.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SH3BP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:2000-1:10000
  • Immunoprecipitation 1:100
  • Western Blot 1:500-1:2000
• Application Notes: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 60 kDa in size corresponding to SH3BP2 by western blotting in the appropriate cell lysate or extract.

Reactivity Notes

This product is phospho specific for human SH3BP2.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SH3BP2 [p Ser427] Antibody - BSA Free

• Abl-SH3 binding protein 2
• Cherubism
• CRBM
• CRPM
• FLJ42079
• FLJ54978,3BP-2,3BP2
• RES4-23
• SH3 domain-binding protein 2
• SH3-domain binding protein 2
• TNFAIP3 interacting protein 2

Background

SH3BP2 binds differentially to the SH3 domains of certain proteins of signal transduction pathways. It also binds to phosphatidylinositols, thereby linking the hemopoietic tyrosine kinase, fes, to the cytoplasmic membrane in a phosphorylation dependent mechanism. Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SH3BP2
• Uniprot:
  • P78314(Human)