ZAP70 Antibody (2F3.2) - IHC-Prediluted

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC-P
Clone
2F3.2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

ZAP70 Antibody (2F3.2) - IHC-Prediluted Summary

Immunogen
Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70
Localization
Cytoplasmic
Marker
Chronic Lymphocytic Leukemia Marker
Specificity
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
ZAP70
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
Application Notes
Hu-chromosome location: 2q12 Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10 - 20 min followed by cooling at RT for 20 minutes. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
1.0mM PBS and 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Alternate Names for ZAP70 Antibody (2F3.2) - IHC-Prediluted

  • 70 kDa zeta-associated protein
  • EC 2.7.10
  • EC 2.7.10.2
  • mrtle
  • mur
  • SRK
  • SRKFLJ17679
  • STD
  • STDFLJ17670
  • Syk-related tyrosine kinase
  • tyrosine-protein kinase ZAP-70
  • TZK
  • ZAP70
  • ZAP-70
  • zeta-chain (TCR) associated protein kinase (70 kD)
  • zeta-chain (TCR) associated protein kinase 70kDa
  • zeta-chain associated protein kinase, 70kD

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 NBP2-47774AF405
Alexa Fluor 488 NBP2-47774AF488
Alexa Fluor 647 NBP2-47774AF647
Alexa Fluor 700 NBP2-47774AF700
Allophycocyanin NBP2-47774APC
Biotin NBP2-47774B
DyLight 350 NBP2-47774UV
DyLight 405 NBP2-47774V
DyLight 488 NBP2-47774G
DyLight 550 NBP2-47774R
DyLight 650 NBP2-47774C
DyLight 680 NBP2-47774FR
DyLight 755 NBP2-47774IR
FITC NBP2-47774F
HRP NBP2-47774H
Janelia Fluor 549 NBP2-47774JF549
Janelia Fluor 646 NBP2-47774JF646
PE NBP2-47774PE
PerCP NBP2-47774PCP

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Bioinformatics

Gene Symbol ZAP70