Vav-1 Antibody (2E11) - BSA Free

Western Blot: Vav-1 Antibody (2E11) [NBP2-37469] - Western blot analysis using VAV1 mouse mAb against Jurkat (1) cell lysate.

Flow Cytometry: Vav-1 Antibody (2E11) [NBP2-37469] - Flow cytometric analysis of HeLa cells using VAV1 mouse mAb (green) and negative control (red).

Western Blot: Vav-1 Antibody (2E11) [NBP2-37469] - Western blot analysis using VAV1 mAb against human VAV1 recombinant protein. (Expected MW is 49.3 kDa)

Western Blot: Vav-1 Antibody (2E11) [NBP2-37469] - Western blot analysis using VAV1 mAb against HEK293 (1) and VAV1 (AA: 121-324)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: Vav-1 Antibody (2E11) [NBP2-37469] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu
• Applications: WB, ELISA, Flow, CyTOF-ready
• Clone: 2E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Vav-1 Antibody (2E11) - BSA Free Summary

• Description: Novus Biologicals Mouse Vav-1 Antibody (2E11) - BSA Free (NBP2-37469) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified recombinant fragment of human Vav-1 (AA: 121-324) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: VAV1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Western Blot 1:250
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 98.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Vav-1 Antibody (2E11) - BSA Free

• oncogene vav
• proto-oncogene vav
• vav 1 guanine nucleotide exchange factor
• vav proto-oncogene
• Vav1
• Vav-1
• vav-T
• VAVvav 1 oncogene

Background

The Vav family are Rho/Rac guanosine nucleotide exchange factors (GEFs), consisting of three members in mammalian cells (Vav, Vav2, Vav3) and one in nematodes (CelVav) (1). First discovered based on its transforming properties, Vav is expressed mainly in hematopoietic cells and a few non-hematopoietic tissues, such as the pancreas and tooth enamels (2). As a signaling transducer, Vav is involved in T-cell activated transduction of T-cell antigen receptor (TCR). T-cell stimulated and tyrosine phosphorylated Vav acts as a catalyst in the exchange of guanosine nucleotides on Rac-1, a GTP binding protein (3). Using a mouse model, Vav expression has been determined to play an essential role in the cyctosketetal, proliferative, and apoptotic pathways for developing lymphoid cells and its signal response (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: VAV1