SYK Antibody

Images

 
Western Blot: SYK Antibody [NBP1-32945] - Mouse tissue extracts (50 ug) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with SYK diluted by 1:1000.
Immunocytochemistry/ Immunofluorescence: SYK Antibody [NBP1-32945] - Analysis of methanol-fixed A431, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded rat spleen dilution: 1:500.
Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: BMDM (bone marrow-derived macrophage) cell B: Syk null cell 10% SDS PAGE; antibody diluted at 1:1000.
Western Blot: SYK Antibody [NBP1-32945] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded MDA-MB468 xenograft, using antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human cervical cancer diluted at 1:500.
Immunohistochemistry-Paraffin: SYK Antibody [NBP1-32945] - Paraffin-embedded human endometrium diluted at 1:500.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.32 mg/ml

Order Details

SYK Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human SYK. The exact sequence is proprietary.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SYK
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:500
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Bovine (80%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
0.32 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SYK Antibody

  • DKFZp313N1010
  • EC 2.7.10
  • EC 2.7.10.2
  • FLJ25043
  • spleen tyrosine kinaseFLJ37489
  • SYK
  • tyrosine-protein kinase SYK

Background

Syk, a non-receptor tyrosine kinase, belongs to the Syk-Zap70 family of protein tyrosine kinase (PTK) and is involved in a wide variety of cellular functions including the pathogenesis of malignant cancer (1). Ubiquitously expressed in hematopoietic cells, Syk has been found to be an effector of B cell receptor (BCR) and functions in B & T cell lymphopoiesis. In the absence of Syk, B cell development is blocked (2). Upon binding to the BCR and to the cell surface receptor of hematopoietic cells (3), Syk is activated and phosphorylates Phospholipase C resulting in the downstream activation of ERK & JNK kinase. Syk also phosphorylates PI3-K activating the Akt pathway (4). Cross-linking of the T cell antigen receptor (TCR)-CD3 complex induces rapid tyrosine phosphorylation and then activation of Syk, which in turn phosphorylates a multitude of intracellular substrates such as Cbl (5). Substitution of Tyr-525 and Tyr-526 at the autophosphorylation site of Syk in mCD8-Syk substantially reduced the kinase activity and the binding to PLC-gamma1 SH2(C) in vitro (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SYK
Entrez